Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
Lipase from wheat germ was immobilized by covalent binding on a nylon -6- grafted with PGMA. This polymer was successfully activated with diethyl amine to intact with lipase enzyme molecule. Response surface methodology (RSM) was applied to model and optimize immobilization conditions for maximum...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Kulliyyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47871/ http://irep.iium.edu.my/47871/1/47871_Optimization_of_Lipase_Immobilization.pdf |
| _version_ | 1848783216573415424 |
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| author | Bahrudin, Nik Adlin Al-Khatib, Ma'an Fahmi Rashid Nasef, Mohamed Mahmoud |
| author2 | Haneef, Sayed Sikandar Shah |
| author_facet | Haneef, Sayed Sikandar Shah Bahrudin, Nik Adlin Al-Khatib, Ma'an Fahmi Rashid Nasef, Mohamed Mahmoud |
| author_sort | Bahrudin, Nik Adlin |
| building | IIUM Repository |
| collection | Online Access |
| description | Lipase from wheat germ was immobilized by covalent binding on a nylon -6- grafted with PGMA. This
polymer was successfully activated with diethyl amine to intact with lipase enzyme molecule. Response
surface methodology (RSM) was applied to model and optimize immobilization conditions for maximum
activity and understanding the interaction of the factors affecting the activity of the immobilized enzyme. The
face centered central composite design (FCCCD) was applied to measure the effect of reaction pH (6-8),
reaction time (2-10 h) and enzyme concentration (1-2 mg) on enzyme activity. The result showed that pH,
time and enzyme concentration had significant effects on the activity of lipase enzyme. Based on the analysis,
the optimum immobilization conditions obtained were at pH 7, immobilization time 5 hours and enzyme
concentration 0.90 mg/ml.
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| first_indexed | 2025-11-14T16:17:50Z |
| format | Proceeding Paper |
| id | iium-47871 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:17:50Z |
| publishDate | 2016 |
| publisher | Kulliyyah of Engineering, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-478712024-05-06T07:07:24Z http://irep.iium.edu.my/47871/ Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer Bahrudin, Nik Adlin Al-Khatib, Ma'an Fahmi Rashid Nasef, Mohamed Mahmoud TP248.13 Biotechnology Lipase from wheat germ was immobilized by covalent binding on a nylon -6- grafted with PGMA. This polymer was successfully activated with diethyl amine to intact with lipase enzyme molecule. Response surface methodology (RSM) was applied to model and optimize immobilization conditions for maximum activity and understanding the interaction of the factors affecting the activity of the immobilized enzyme. The face centered central composite design (FCCCD) was applied to measure the effect of reaction pH (6-8), reaction time (2-10 h) and enzyme concentration (1-2 mg) on enzyme activity. The result showed that pH, time and enzyme concentration had significant effects on the activity of lipase enzyme. Based on the analysis, the optimum immobilization conditions obtained were at pH 7, immobilization time 5 hours and enzyme concentration 0.90 mg/ml. Kulliyyah of Engineering, International Islamic University Malaysia Haneef, Sayed Sikandar Shah Mohd Zin, Najibah Mahmud , Mek Wok 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/47871/1/47871_Optimization_of_Lipase_Immobilization.pdf Bahrudin, Nik Adlin and Al-Khatib, Ma'an Fahmi Rashid and Nasef, Mohamed Mahmoud (2016) Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/ |
| spellingShingle | TP248.13 Biotechnology Bahrudin, Nik Adlin Al-Khatib, Ma'an Fahmi Rashid Nasef, Mohamed Mahmoud Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer |
| title | Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
|
| title_full | Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
|
| title_fullStr | Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
|
| title_full_unstemmed | Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
|
| title_short | Optimization of lipase immobilization on nylon -6-grafted with PGMA polymer
|
| title_sort | optimization of lipase immobilization on nylon -6-grafted with pgma polymer |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/47871/ http://irep.iium.edu.my/47871/ http://irep.iium.edu.my/47871/1/47871_Optimization_of_Lipase_Immobilization.pdf |