DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene
This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or white threads fis...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/47379/ http://irep.iium.edu.my/47379/1/43579.pdf |
| _version_ | 1848783134128078848 |
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| author | K., Kamarudin Mohamed Rehan, Maryam Ramly, Nur Zazarina Mohamed Rehan, Aisyah |
| author_facet | K., Kamarudin Mohamed Rehan, Maryam Ramly, Nur Zazarina Mohamed Rehan, Aisyah |
| author_sort | K., Kamarudin |
| building | IIUM Repository |
| collection | Online Access |
| description | This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or
gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or
dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt,
1835) or white threads fish from Pangkor Island, Perak Darul Ridzuan, Malaysia applying
partial DNA sequencing of non-protein-coding 16S mitochondrial ribosomal RNA gene.
Pangkor Island in the north region of Peninsular Malaysia is well known for its traditional
production industry of air gamat (body fluid extracts) and minyak gamat (lipid extracts). The
phylogenetic analyses using Neighbour-Joining method and Maximum Likelihood method
supported the morphospecies status of H. leucospilota and S. horrens. Despite the fact that more
specimens and different molecular approaches are required to produce better conclusive results,
the current findings gave a better insight of the importance of morphological approach and 16S
mitochondrial rRNA gene sequencing in identification of the two Malaysian sea cucumber
species from Pangkor Island. Furthermore, the gene barcodes can be used as the commercial
species standard or reference samples in determining the species identities of processed sea
cucumbers from Malaysian markets. |
| first_indexed | 2025-11-14T16:16:31Z |
| format | Proceeding Paper |
| id | iium-47379 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:16:31Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-473792016-05-24T05:26:32Z http://irep.iium.edu.my/47379/ DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene K., Kamarudin Mohamed Rehan, Maryam Ramly, Nur Zazarina Mohamed Rehan, Aisyah Q Science (General) This study aimed to barcode a commercial sea cucumber (Echinodermata: Holothuroidea) or gamat species i.e., Stichopus horrens Selenka, 1867Error! Reference source not found. or dragonfish, and a timun laut species i.e., Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or white threads fish from Pangkor Island, Perak Darul Ridzuan, Malaysia applying partial DNA sequencing of non-protein-coding 16S mitochondrial ribosomal RNA gene. Pangkor Island in the north region of Peninsular Malaysia is well known for its traditional production industry of air gamat (body fluid extracts) and minyak gamat (lipid extracts). The phylogenetic analyses using Neighbour-Joining method and Maximum Likelihood method supported the morphospecies status of H. leucospilota and S. horrens. Despite the fact that more specimens and different molecular approaches are required to produce better conclusive results, the current findings gave a better insight of the importance of morphological approach and 16S mitochondrial rRNA gene sequencing in identification of the two Malaysian sea cucumber species from Pangkor Island. Furthermore, the gene barcodes can be used as the commercial species standard or reference samples in determining the species identities of processed sea cucumbers from Malaysian markets. 2015-10-13 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/47379/1/43579.pdf K., Kamarudin and Mohamed Rehan, Maryam and Ramly, Nur Zazarina and Mohamed Rehan, Aisyah (2015) DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene. In: Kolokium Siswazah Sains dan Teknologi (KoSiST'15), 13th Oct. 2015, Akademi Kepimpinan Pengajian Tinggi (AKEPT), Bandar Enstek, Negeri Sembilan. http://pcfst.usim.edu.my/ |
| spellingShingle | Q Science (General) K., Kamarudin Mohamed Rehan, Maryam Ramly, Nur Zazarina Mohamed Rehan, Aisyah DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene |
| title | DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene |
| title_full | DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene |
| title_fullStr | DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene |
| title_full_unstemmed | DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene |
| title_short | DNA barcoding of gamat and timun laut species from Pangkor Island using 16S mitochondrial ribosomal RNA gene |
| title_sort | dna barcoding of gamat and timun laut species from pangkor island using 16s mitochondrial ribosomal rna gene |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/47379/ http://irep.iium.edu.my/47379/ http://irep.iium.edu.my/47379/1/43579.pdf |