Electrochemical sensor centrifuge platform for single-cell study
SporeSat – a lab-on-a-chip (LOC) centrifuge platform designed for integration as the payload of a small (5.5 kg), free-flying satellite – has been developed to determine the gravitational thresholds for calcium-ion channel activation of a single-cell spore from the fern Ceratopteris richardii. This...
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| Format: | Proceeding Paper |
| Language: | English English English English |
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2015
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| Online Access: | http://irep.iium.edu.my/47224/ http://irep.iium.edu.my/47224/1/ACB2015_Abstract_Dr_Wan_Wardatul_Amani_Wan_Salim.pdf http://irep.iium.edu.my/47224/4/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim.pdf http://irep.iium.edu.my/47224/5/ACB_2015_Dr_Amani.pdf http://irep.iium.edu.my/47224/6/ACB_2015_presentation.pdf |
| _version_ | 1848783107376807936 |
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| author | Wan Salim, Wan Wardatul Amani |
| author_facet | Wan Salim, Wan Wardatul Amani |
| author_sort | Wan Salim, Wan Wardatul Amani |
| building | IIUM Repository |
| collection | Online Access |
| description | SporeSat – a lab-on-a-chip (LOC) centrifuge platform designed for integration as the payload of a small (5.5 kg), free-flying satellite – has been developed to determine the gravitational thresholds for calcium-ion channel activation of a single-cell spore from the fern Ceratopteris richardii. This fern is an important model system for gravity-directed plant-cell development during variable-gravity conditions attainable only in space flight. Calcium-ion channel activity is measured by photolithographically defined calcium ion–selective electrodes (ISEs) at opposite ends of each spore. Artificial gravity is created by rotating a disk-like platform that contains the spores in wells along with the calcium ISEs. Ground experiments reveal a maximum calcium concentration ratio at 2.2xg, between micro-ion-selective electrodes near the “top” and “bottom” ends of the spore, indicating an increasing calcium concentration at one “end” of the fern spore with respect to the other. Confocal micrographs of rhizoid formation confirm the light-induced germination. SporeSat is a spaceflight experiment that will take ~ 4 days; data will be telemetered to Earth over ~ 100 days. |
| first_indexed | 2025-11-14T16:16:06Z |
| format | Proceeding Paper |
| id | iium-47224 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English English |
| last_indexed | 2025-11-14T16:16:06Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-472242016-05-20T06:29:36Z http://irep.iium.edu.my/47224/ Electrochemical sensor centrifuge platform for single-cell study Wan Salim, Wan Wardatul Amani S Agriculture (General) TP248.13 Biotechnology TP250 Industrial electrochemistry SporeSat – a lab-on-a-chip (LOC) centrifuge platform designed for integration as the payload of a small (5.5 kg), free-flying satellite – has been developed to determine the gravitational thresholds for calcium-ion channel activation of a single-cell spore from the fern Ceratopteris richardii. This fern is an important model system for gravity-directed plant-cell development during variable-gravity conditions attainable only in space flight. Calcium-ion channel activity is measured by photolithographically defined calcium ion–selective electrodes (ISEs) at opposite ends of each spore. Artificial gravity is created by rotating a disk-like platform that contains the spores in wells along with the calcium ISEs. Ground experiments reveal a maximum calcium concentration ratio at 2.2xg, between micro-ion-selective electrodes near the “top” and “bottom” ends of the spore, indicating an increasing calcium concentration at one “end” of the fern spore with respect to the other. Confocal micrographs of rhizoid formation confirm the light-induced germination. SporeSat is a spaceflight experiment that will take ~ 4 days; data will be telemetered to Earth over ~ 100 days. 2015-11-19 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/47224/1/ACB2015_Abstract_Dr_Wan_Wardatul_Amani_Wan_Salim.pdf application/pdf en http://irep.iium.edu.my/47224/4/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim.pdf application/pdf en http://irep.iium.edu.my/47224/5/ACB_2015_Dr_Amani.pdf application/pdf en http://irep.iium.edu.my/47224/6/ACB_2015_presentation.pdf Wan Salim, Wan Wardatul Amani (2015) Electrochemical sensor centrifuge platform for single-cell study. In: 12th Asian Congress on Biotechnology (ACB 2015) , 15-19 November 2015, Hotel Istana, Kuala Lumpur. |
| spellingShingle | S Agriculture (General) TP248.13 Biotechnology TP250 Industrial electrochemistry Wan Salim, Wan Wardatul Amani Electrochemical sensor centrifuge platform for single-cell study |
| title | Electrochemical sensor centrifuge platform for
single-cell study
|
| title_full | Electrochemical sensor centrifuge platform for
single-cell study
|
| title_fullStr | Electrochemical sensor centrifuge platform for
single-cell study
|
| title_full_unstemmed | Electrochemical sensor centrifuge platform for
single-cell study
|
| title_short | Electrochemical sensor centrifuge platform for
single-cell study
|
| title_sort | electrochemical sensor centrifuge platform for
single-cell study |
| topic | S Agriculture (General) TP248.13 Biotechnology TP250 Industrial electrochemistry |
| url | http://irep.iium.edu.my/47224/ http://irep.iium.edu.my/47224/1/ACB2015_Abstract_Dr_Wan_Wardatul_Amani_Wan_Salim.pdf http://irep.iium.edu.my/47224/4/ACB_2015_acceptance_Dr._Wan_Wardatul_Amani_Wan_Salim.pdf http://irep.iium.edu.my/47224/5/ACB_2015_Dr_Amani.pdf http://irep.iium.edu.my/47224/6/ACB_2015_presentation.pdf |