Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE)
Amlodipine is antihypertensive drug belonging to calcium antagonist group. This study aims to apply experimental design for the optimization of extraction method of amlodipine from human plasma for higher and constant recovery. A chromatographic method involving HPLC (High Performance Liquid Chroma...
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| Format: | Proceeding Paper |
| Language: | English |
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2015
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| Online Access: | http://irep.iium.edu.my/46618/ http://irep.iium.edu.my/46618/1/IPNaCS_2015_%28Development_and_validation_of_bioanalytical_method_for_amlodipine_in_human_plasma_with_optimised_extraction_method_using_DOE.pdf |
| _version_ | 1848782998810394624 |
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| author | Helal Uddin, A.B.M. Alaama, Mohammed Mohamad, Huda Jamilah Amirudin, N. S. |
| author_facet | Helal Uddin, A.B.M. Alaama, Mohammed Mohamad, Huda Jamilah Amirudin, N. S. |
| author_sort | Helal Uddin, A.B.M. |
| building | IIUM Repository |
| collection | Online Access |
| description | Amlodipine is antihypertensive drug belonging to calcium antagonist group. This study aims to apply experimental design for the optimization of extraction method of amlodipine from human plasma for higher and constant recovery. A chromatographic method involving HPLC (High Performance Liquid Chromatography) with DAD (Diode Array Detector) was developed and validated for the determination of Amlodipine in human plasma with cetirizine as internal standard. Sample preparation for the extraction of Amlodipine and Cetirizine (Internal Standard) from plasma was optimised using DOE (Design of Experiment).
Three different parameters namely solvent type, solvent volume and pH were monitored at different level for the optimisation of sample preparation that would produce best recovery of the analytes. For this purpose, three level full factorial design, which involves 27 experiments, was conducted. The recovery of amlodipine was determined using the developed and pre validated HPLC-DAD method with an Agilent 1100 HPLC system. Chromatographic separation of the analytes was obtained using phenomenix (150X4.5 mm, 5 um) C18 column. The mobile phase consists of 35:65 v/v of ACN: 0.3% triethylamine with pH adjusted at 3 using phosphoric acid. Detection of analyte was conducted using a diod array detector at 237 nm wavelength. Design Expert 8.0 software was used to interpret all data obtained from the optimisation study. For the parameters solvent and solvent volume “acetonitrile” and “1 ml” was found to be the optimum values required to extract amlodipine and internal standard while pH had no effect on the optimum recovery. The recovery of amlodipine and internal standard was 80-88% and 85-99% respectively.
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| first_indexed | 2025-11-14T16:14:22Z |
| format | Proceeding Paper |
| id | iium-46618 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:14:22Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-466182022-11-07T03:54:18Z http://irep.iium.edu.my/46618/ Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE) Helal Uddin, A.B.M. Alaama, Mohammed Mohamad, Huda Jamilah Amirudin, N. S. QD Chemistry RS Pharmacy and materia medica Amlodipine is antihypertensive drug belonging to calcium antagonist group. This study aims to apply experimental design for the optimization of extraction method of amlodipine from human plasma for higher and constant recovery. A chromatographic method involving HPLC (High Performance Liquid Chromatography) with DAD (Diode Array Detector) was developed and validated for the determination of Amlodipine in human plasma with cetirizine as internal standard. Sample preparation for the extraction of Amlodipine and Cetirizine (Internal Standard) from plasma was optimised using DOE (Design of Experiment). Three different parameters namely solvent type, solvent volume and pH were monitored at different level for the optimisation of sample preparation that would produce best recovery of the analytes. For this purpose, three level full factorial design, which involves 27 experiments, was conducted. The recovery of amlodipine was determined using the developed and pre validated HPLC-DAD method with an Agilent 1100 HPLC system. Chromatographic separation of the analytes was obtained using phenomenix (150X4.5 mm, 5 um) C18 column. The mobile phase consists of 35:65 v/v of ACN: 0.3% triethylamine with pH adjusted at 3 using phosphoric acid. Detection of analyte was conducted using a diod array detector at 237 nm wavelength. Design Expert 8.0 software was used to interpret all data obtained from the optimisation study. For the parameters solvent and solvent volume “acetonitrile” and “1 ml” was found to be the optimum values required to extract amlodipine and internal standard while pH had no effect on the optimum recovery. The recovery of amlodipine and internal standard was 80-88% and 85-99% respectively. 2015 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/46618/1/IPNaCS_2015_%28Development_and_validation_of_bioanalytical_method_for_amlodipine_in_human_plasma_with_optimised_extraction_method_using_DOE.pdf Helal Uddin, A.B.M. and Alaama, Mohammed and Mohamad, Huda Jamilah and Amirudin, N. S. (2015) Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE). In: 4th International Conference on Pharmaceuticals, Nutraceuticals and Cosmetic Science (IPNaCs), 12th-13th Nov. 2015, Ramada Plaza, Malaka, Malaysia. http://ipnacs.uitm.edu.my/ |
| spellingShingle | QD Chemistry RS Pharmacy and materia medica Helal Uddin, A.B.M. Alaama, Mohammed Mohamad, Huda Jamilah Amirudin, N. S. Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE) |
| title | Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE) |
| title_full | Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE) |
| title_fullStr | Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE) |
| title_full_unstemmed | Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE) |
| title_short | Development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using Design of Experiment (DOE) |
| title_sort | development and validation of bioanalytical method for amlodipine in human plasma with optimised extraction method using design of experiment (doe) |
| topic | QD Chemistry RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/46618/ http://irep.iium.edu.my/46618/ http://irep.iium.edu.my/46618/1/IPNaCS_2015_%28Development_and_validation_of_bioanalytical_method_for_amlodipine_in_human_plasma_with_optimised_extraction_method_using_DOE.pdf |