Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability
This study aimed to investigate the effects of the ultrasound-assisted extraction conditions on the yield, specific activity, temperature, and storage stability of the pectinase enzyme from guava peel. The ultrasound variables studied were sonication time (10-30min), ultrasound temperature (30-50 de...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English English |
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Taylor and Francis Ltd.
2016
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| Online Access: | http://irep.iium.edu.my/46544/ http://irep.iium.edu.my/46544/1/46544_Optimization%20of%20ultrasound-assisted%20extraction.pdf http://irep.iium.edu.my/46544/2/55692_Duplex%20real-time%20PCR%20assay%20using%20SYBR_SCOPUS.pdf http://irep.iium.edu.my/46544/3/55692_Duplex%20real-time%20PCR%20assay%20using%20SYBR_WOS.pdf |
| _version_ | 1848782987283398656 |
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| author | Amid, Mehrnoush Murshid, Fara Syazana Manap, Mohd Yazid Sarker, Md. Zaidul Islam |
| author_facet | Amid, Mehrnoush Murshid, Fara Syazana Manap, Mohd Yazid Sarker, Md. Zaidul Islam |
| author_sort | Amid, Mehrnoush |
| building | IIUM Repository |
| collection | Online Access |
| description | This study aimed to investigate the effects of the ultrasound-assisted extraction conditions on the yield, specific activity, temperature, and storage stability of the pectinase enzyme from guava peel. The ultrasound variables studied were sonication time (10-30min), ultrasound temperature (30-50 degrees C), pH (2.0-8.0), and solvent-to-sample ratio (2:1mL/g to 6:1mL/g). The main goal was to optimize the ultrasound-assisted extraction conditions to maximize the recovery of pectinase from guava peel with the most desirable enzyme-specific activity and stability. Under the optimum conditions, a high yield (96.2%), good specific activity (18.2U/mg), temperature stability (88.3%), and storage stability (90.3%) of the extracted enzyme were achieved. The optimal conditions were 20min sonication time, 40 degrees C temperature, at pH 5.0, using a 4:1mL/g solvent-to-sample ratio. The study demonstrated that optimization of ultrasound-assisted process conditions for the enzyme extraction could improve the enzymatic characteristics and yield of the enzyme. |
| first_indexed | 2025-11-14T16:14:11Z |
| format | Article |
| id | iium-46544 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T16:14:11Z |
| publishDate | 2016 |
| publisher | Taylor and Francis Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-465442017-10-12T02:39:22Z http://irep.iium.edu.my/46544/ Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability Amid, Mehrnoush Murshid, Fara Syazana Manap, Mohd Yazid Sarker, Md. Zaidul Islam Q Science (General) T Technology (General) This study aimed to investigate the effects of the ultrasound-assisted extraction conditions on the yield, specific activity, temperature, and storage stability of the pectinase enzyme from guava peel. The ultrasound variables studied were sonication time (10-30min), ultrasound temperature (30-50 degrees C), pH (2.0-8.0), and solvent-to-sample ratio (2:1mL/g to 6:1mL/g). The main goal was to optimize the ultrasound-assisted extraction conditions to maximize the recovery of pectinase from guava peel with the most desirable enzyme-specific activity and stability. Under the optimum conditions, a high yield (96.2%), good specific activity (18.2U/mg), temperature stability (88.3%), and storage stability (90.3%) of the extracted enzyme were achieved. The optimal conditions were 20min sonication time, 40 degrees C temperature, at pH 5.0, using a 4:1mL/g solvent-to-sample ratio. The study demonstrated that optimization of ultrasound-assisted process conditions for the enzyme extraction could improve the enzymatic characteristics and yield of the enzyme. Taylor and Francis Ltd. 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46544/1/46544_Optimization%20of%20ultrasound-assisted%20extraction.pdf application/pdf en http://irep.iium.edu.my/46544/2/55692_Duplex%20real-time%20PCR%20assay%20using%20SYBR_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/46544/3/55692_Duplex%20real-time%20PCR%20assay%20using%20SYBR_WOS.pdf Amid, Mehrnoush and Murshid, Fara Syazana and Manap, Mohd Yazid and Sarker, Md. Zaidul Islam (2016) Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability. Preparative Biochemistry and Biotechnology, 46 (1). pp. 91-99. ISSN 1082-6068 E-ISSN 1532-2297 http://www.tandfonline.com/doi/abs/10.1080/10826068.2015.1031396?journalCode=lpbb20 10.1080/10826068.2015.1031396 |
| spellingShingle | Q Science (General) T Technology (General) Amid, Mehrnoush Murshid, Fara Syazana Manap, Mohd Yazid Sarker, Md. Zaidul Islam Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability |
| title | Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability |
| title_full | Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability |
| title_fullStr | Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability |
| title_full_unstemmed | Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability |
| title_short | Optimization of ultrasound-assisted extraction of pectinase enzyme from guava (Psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability |
| title_sort | optimization of ultrasound-assisted extraction of pectinase enzyme from guava (psidium guajava) peel: enzyme recovery, specific activity, temperature, and storage stability |
| topic | Q Science (General) T Technology (General) |
| url | http://irep.iium.edu.my/46544/ http://irep.iium.edu.my/46544/ http://irep.iium.edu.my/46544/ http://irep.iium.edu.my/46544/1/46544_Optimization%20of%20ultrasound-assisted%20extraction.pdf http://irep.iium.edu.my/46544/2/55692_Duplex%20real-time%20PCR%20assay%20using%20SYBR_SCOPUS.pdf http://irep.iium.edu.my/46544/3/55692_Duplex%20real-time%20PCR%20assay%20using%20SYBR_WOS.pdf |