Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy
Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological prope...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
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UKM Kuala Lumpur Campus
2015
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| Online Access: | http://irep.iium.edu.my/46387/ http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf |
| _version_ | 1848782957998768128 |
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| author | Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah |
| author_facet | Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah |
| author_sort | Doolaanea, Abd Almonem |
| building | IIUM Repository |
| collection | Online Access |
| description | Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological properties. CTAB was found to adsorb on the surface of F1 with easy desorption. This led to negative zeta potential, high cytotoxicity and low cell uptake/transfection. F2 exhibited negative zeta potential, low cytotoxicity, and higher cell uptake/transfection. F3 and F4 showed positive zeta potential, low cytotoxicity and high cell uptake/transfection. Due to its stronger interaction with PLGA that may stabilise the plasmid DNA, F4 exhibited higher transfection efficacy. This study highlights the significant differences between CTAB and chitosan in modifying nanoparticles for gene therapy. |
| first_indexed | 2025-11-14T16:13:43Z |
| format | Proceeding Paper |
| id | iium-46387 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:13:43Z |
| publishDate | 2015 |
| publisher | UKM Kuala Lumpur Campus |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-463872016-05-27T02:43:09Z http://irep.iium.edu.my/46387/ Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah RS Pharmacy and materia medica Poly (D, L-Lactic-co-Glycolic Acid) (PLGA) nanoparticles were modified by adding cetyltrimethylammonium bromide (CTAB) to the aqueous phase (F1) or oil phase (F2), or by low (F3) or medium (F4) molecular weight chitosan. Nanoparticles were characterised for their physicochemical and biological properties. CTAB was found to adsorb on the surface of F1 with easy desorption. This led to negative zeta potential, high cytotoxicity and low cell uptake/transfection. F2 exhibited negative zeta potential, low cytotoxicity, and higher cell uptake/transfection. F3 and F4 showed positive zeta potential, low cytotoxicity and high cell uptake/transfection. Due to its stronger interaction with PLGA that may stabilise the plasmid DNA, F4 exhibited higher transfection efficacy. This study highlights the significant differences between CTAB and chitosan in modifying nanoparticles for gene therapy. UKM Kuala Lumpur Campus 2015 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf application/pdf en http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf Doolaanea, Abd Almonem and Mansor, Nur 'Izzati and Mohd Nor, Nurul Hafizah and Mohamed, Farahidah (2015) Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy. In: Controlled Released & Delivery Symposium 2015 (CRDDS2015), 15th-16th Aug. 2015, Faculty of Pharmacy, Universiti Kebangsaan Malaysia. (Unpublished) |
| spellingShingle | RS Pharmacy and materia medica Doolaanea, Abd Almonem Mansor, Nur 'Izzati Mohd Nor, Nurul Hafizah Mohamed, Farahidah Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy |
| title | Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy |
| title_full | Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy |
| title_fullStr | Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy |
| title_full_unstemmed | Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy |
| title_short | Differences between CTAB and chitosan in modifying PLGA nanoparticles for gene therapy |
| title_sort | differences between ctab and chitosan in modifying plga nanoparticles for gene therapy |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/46387/ http://irep.iium.edu.my/46387/4/Monem_CRDDS2015.pdf http://irep.iium.edu.my/46387/7/46387_Differences_Between_CTAB_Abstract.pdf |