Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification
Nigella sativa oil (NSO) has been exploited for medical purposes for many generations. The fabrication of microparticles containing NSO intended for sustained release was done to be used in treating osteomyelitis. Method in quantifying NSO using UV-spectroscopy was developed and validated. Linearity...
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| Format: | Article |
| Language: | English |
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Open Science Publishers LLP Inc.
2015
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| Online Access: | http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/1/Method_UV_Spectrophotometry_for_Nigella_sativa_Oil.pdf |
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| author | Harun Ismail, Ahmad Fahmi Doolaanea, Abd Almonem Mohamed, Farahidah Mansor, Nur 'Izzati Mohd Shafri, Mohd Affendi Yusof, Fathin Athirah |
| author_facet | Harun Ismail, Ahmad Fahmi Doolaanea, Abd Almonem Mohamed, Farahidah Mansor, Nur 'Izzati Mohd Shafri, Mohd Affendi Yusof, Fathin Athirah |
| author_sort | Harun Ismail, Ahmad Fahmi |
| building | IIUM Repository |
| collection | Online Access |
| description | Nigella sativa oil (NSO) has been exploited for medical purposes for many generations. The fabrication of microparticles containing NSO intended for sustained release was done to be used in treating osteomyelitis. Method in quantifying NSO using UV-spectroscopy was developed and validated. Linearity shown a good correlation coefficient with the values higher than 0.995, both for actual and different analysts. The LOD and LOQ values were recorded to be 2.89 μg/mL and 8.75 μg/mL respectively. In addition, the highest %RSD values for the intermediate and repeatability studies were 0.970% and 0.445% which suggested the method was precise. The percentage recovery for 4 known concentrations gave the range between 98.16% to 99.39%, indicating the high accuracy of the method. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines. |
| first_indexed | 2025-11-14T16:13:25Z |
| format | Article |
| id | iium-46273 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:13:25Z |
| publishDate | 2015 |
| publisher | Open Science Publishers LLP Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-462732017-02-14T03:00:42Z http://irep.iium.edu.my/46273/ Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification Harun Ismail, Ahmad Fahmi Doolaanea, Abd Almonem Mohamed, Farahidah Mansor, Nur 'Izzati Mohd Shafri, Mohd Affendi Yusof, Fathin Athirah RS Pharmacy and materia medica Nigella sativa oil (NSO) has been exploited for medical purposes for many generations. The fabrication of microparticles containing NSO intended for sustained release was done to be used in treating osteomyelitis. Method in quantifying NSO using UV-spectroscopy was developed and validated. Linearity shown a good correlation coefficient with the values higher than 0.995, both for actual and different analysts. The LOD and LOQ values were recorded to be 2.89 μg/mL and 8.75 μg/mL respectively. In addition, the highest %RSD values for the intermediate and repeatability studies were 0.970% and 0.445% which suggested the method was precise. The percentage recovery for 4 known concentrations gave the range between 98.16% to 99.39%, indicating the high accuracy of the method. The parameters analyzed in this study were in accordance with ICH Q2 (R1) guidelines. Open Science Publishers LLP Inc. 2015-09 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/46273/1/Method_UV_Spectrophotometry_for_Nigella_sativa_Oil.pdf Harun Ismail, Ahmad Fahmi and Doolaanea, Abd Almonem and Mohamed, Farahidah and Mansor, Nur 'Izzati and Mohd Shafri, Mohd Affendi and Yusof, Fathin Athirah (2015) Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification. Journal of Applied Pharmaceutical Science, 5 (9). 082-088. ISSN 2231-3354 http://www.japsonline.com/admin/php/uploads/1628_pdf.pdf 10.7324/JAPS.2015.50915 |
| spellingShingle | RS Pharmacy and materia medica Harun Ismail, Ahmad Fahmi Doolaanea, Abd Almonem Mohamed, Farahidah Mansor, Nur 'Izzati Mohd Shafri, Mohd Affendi Yusof, Fathin Athirah Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification |
| title | Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification |
| title_full | Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification |
| title_fullStr | Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification |
| title_full_unstemmed | Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification |
| title_short | Method development and validation using UV spectrophotometry for Nigella sativa oil microparticles quantification |
| title_sort | method development and validation using uv spectrophotometry for nigella sativa oil microparticles quantification |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/ http://irep.iium.edu.my/46273/1/Method_UV_Spectrophotometry_for_Nigella_sativa_Oil.pdf |