The effect of dose on the intensity of dependence in morphine-dependent mice
Introduction: Animal behaviour research is an important tool to study the addiction properties of addictive substances. The Conditioned Place Preference (CPP)paradigm is among the model used to achieve this purpose. It can be used to investigate a wide range of addiction related behaviour including...
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| Format: | Proceeding Paper |
| Language: | English English |
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2015
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| Online Access: | http://irep.iium.edu.my/45949/ http://irep.iium.edu.my/45949/7/PPoint_UCSI_%28S%29.pdf http://irep.iium.edu.my/45949/10/45949.pdf |
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| author | Jusoh, Ahmad Jamaluddin Al-Qasami Syd Mohmad Faudzi, Syed Mohd Syahmi Suhaimi, Maryam Saadah Hashim, Ridzwan Ridzwan, Irna Elina |
| author_facet | Jusoh, Ahmad Jamaluddin Al-Qasami Syd Mohmad Faudzi, Syed Mohd Syahmi Suhaimi, Maryam Saadah Hashim, Ridzwan Ridzwan, Irna Elina |
| author_sort | Jusoh, Ahmad Jamaluddin Al-Qasami |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Animal behaviour research is an important tool to study the addiction properties of addictive substances. The Conditioned Place Preference (CPP)paradigm is among the model used to achieve this purpose. It can be used to investigate a wide range of addiction related behaviour including intoxication and relapse potential. Since it involves a long cycle experiment, it is important to optimise the experimental conditions, but minimising any unnecessity. Therefore, in this study we aim to investigate the effects of different morphine dose (3.75 - 10 mg/kg) towards intensity of morphine dependence in adult Swiss albino mice.
Methods: The mice were divided into four group (n=10-12 for each group), where each group received different doses of morphine and saline on alternate day for 6 days during conditioning phase. The intensity of dependence was measured during post-conditioning phase where the preference at the drug-paired compartment was calculated during a 15 minutes observation.
Results: The result has shown that three out of four groups significantly developed dependence (P < 0.05). However, the group of mice that received highest morphine dose (10 mg/kg) did not develop dependence. When baseline was compared, it was found that the mice that received the lowest morphine treatment (3.75 mg/kg) shows the highest increment from their baseline (164.7±33.3 seconds), while the group that received 10 mg/kg morphine shows the lowest increment (119.0±31.6 seconds). The trend of increment at the drug-paired compartment shows a declining trend when the dose of morphine is increased.
Conclusion: Therefore, it is concluded that the optimum dose of morphine that should be administered to the adult Swiss albino mice is around 3.75 mg/kg, provided that all other experimental conditions and protocols are maintained. |
| first_indexed | 2025-11-14T16:12:27Z |
| format | Proceeding Paper |
| id | iium-45949 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:12:27Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-459492015-12-18T00:37:57Z http://irep.iium.edu.my/45949/ The effect of dose on the intensity of dependence in morphine-dependent mice Jusoh, Ahmad Jamaluddin Al-Qasami Syd Mohmad Faudzi, Syed Mohd Syahmi Suhaimi, Maryam Saadah Hashim, Ridzwan Ridzwan, Irna Elina RS Pharmacy and materia medica Introduction: Animal behaviour research is an important tool to study the addiction properties of addictive substances. The Conditioned Place Preference (CPP)paradigm is among the model used to achieve this purpose. It can be used to investigate a wide range of addiction related behaviour including intoxication and relapse potential. Since it involves a long cycle experiment, it is important to optimise the experimental conditions, but minimising any unnecessity. Therefore, in this study we aim to investigate the effects of different morphine dose (3.75 - 10 mg/kg) towards intensity of morphine dependence in adult Swiss albino mice. Methods: The mice were divided into four group (n=10-12 for each group), where each group received different doses of morphine and saline on alternate day for 6 days during conditioning phase. The intensity of dependence was measured during post-conditioning phase where the preference at the drug-paired compartment was calculated during a 15 minutes observation. Results: The result has shown that three out of four groups significantly developed dependence (P < 0.05). However, the group of mice that received highest morphine dose (10 mg/kg) did not develop dependence. When baseline was compared, it was found that the mice that received the lowest morphine treatment (3.75 mg/kg) shows the highest increment from their baseline (164.7±33.3 seconds), while the group that received 10 mg/kg morphine shows the lowest increment (119.0±31.6 seconds). The trend of increment at the drug-paired compartment shows a declining trend when the dose of morphine is increased. Conclusion: Therefore, it is concluded that the optimum dose of morphine that should be administered to the adult Swiss albino mice is around 3.75 mg/kg, provided that all other experimental conditions and protocols are maintained. 2015-10-10 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/45949/7/PPoint_UCSI_%28S%29.pdf application/pdf en http://irep.iium.edu.my/45949/10/45949.pdf Jusoh, Ahmad Jamaluddin Al-Qasami and Syd Mohmad Faudzi, Syed Mohd Syahmi and Suhaimi, Maryam Saadah and Hashim, Ridzwan and Ridzwan, Irna Elina (2015) The effect of dose on the intensity of dependence in morphine-dependent mice. In: Health and Pharmaceutical Sciences Undergraduate Research Symposium, 10th October, 2015, Le Quadri Ball Room, KL campus, North Wing, UCSI University, KL, Malaysia. (Unpublished) |
| spellingShingle | RS Pharmacy and materia medica Jusoh, Ahmad Jamaluddin Al-Qasami Syd Mohmad Faudzi, Syed Mohd Syahmi Suhaimi, Maryam Saadah Hashim, Ridzwan Ridzwan, Irna Elina The effect of dose on the intensity of dependence in morphine-dependent mice |
| title | The effect of dose on the intensity of dependence in morphine-dependent mice |
| title_full | The effect of dose on the intensity of dependence in morphine-dependent mice |
| title_fullStr | The effect of dose on the intensity of dependence in morphine-dependent mice |
| title_full_unstemmed | The effect of dose on the intensity of dependence in morphine-dependent mice |
| title_short | The effect of dose on the intensity of dependence in morphine-dependent mice |
| title_sort | effect of dose on the intensity of dependence in morphine-dependent mice |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/45949/ http://irep.iium.edu.my/45949/7/PPoint_UCSI_%28S%29.pdf http://irep.iium.edu.my/45949/10/45949.pdf |