Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
Development and validation of an LC-MS/MS method suitable for the simultaneous quantification of phenolic acids and flavonoids is described. Analytes were separated on a C6-phenyl column, analysed using negative ion APCI selected reaction monitoring. The method is capable of analysing 14 compounds...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/45944/ http://irep.iium.edu.my/45944/4/45944.pdf |
| _version_ | 1848782876743565312 |
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| author | Haron, Normah Arcot, Jayashree Bucknall, Martin |
| author_facet | Haron, Normah Arcot, Jayashree Bucknall, Martin |
| author_sort | Haron, Normah |
| building | IIUM Repository |
| collection | Online Access |
| description | Development and validation of an LC-MS/MS method suitable for the simultaneous quantification of phenolic acids and flavonoids is described. Analytes were separated on a C6-phenyl column, analysed using negative ion APCI selected reaction monitoring. The method is capable of analysing 14 compounds (9 phenolic acids and 5 flavonoids) in 85 minutes and uses deuterium-labelled 4-hydroxy-3-methoxy-d3-cinnamic acid (ferulic acid-d3) and 13C15-quercetin as internal standards. The results demonstrated that the method developed was reliable, specific and robust. Thus the method was successfully applied to analyse phenolic compounds in 18 different types of traditional Malaysian vegetables (both raw and processed) with 3 different fractions of phenolic acids. Gallic, ferulic, vanillic and caffeic acids were the most dominant phenolic acids, while quercetin, apigenin, kaempferol and luteolin were the predominant flavonoids in the samples studied.
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| first_indexed | 2025-11-14T16:12:26Z |
| format | Proceeding Paper |
| id | iium-45944 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:12:26Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-459442017-12-05T08:03:36Z http://irep.iium.edu.my/45944/ Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables Haron, Normah Arcot, Jayashree Bucknall, Martin Q Science (General) S Agriculture (General) Development and validation of an LC-MS/MS method suitable for the simultaneous quantification of phenolic acids and flavonoids is described. Analytes were separated on a C6-phenyl column, analysed using negative ion APCI selected reaction monitoring. The method is capable of analysing 14 compounds (9 phenolic acids and 5 flavonoids) in 85 minutes and uses deuterium-labelled 4-hydroxy-3-methoxy-d3-cinnamic acid (ferulic acid-d3) and 13C15-quercetin as internal standards. The results demonstrated that the method developed was reliable, specific and robust. Thus the method was successfully applied to analyse phenolic compounds in 18 different types of traditional Malaysian vegetables (both raw and processed) with 3 different fractions of phenolic acids. Gallic, ferulic, vanillic and caffeic acids were the most dominant phenolic acids, while quercetin, apigenin, kaempferol and luteolin were the predominant flavonoids in the samples studied. 2015-11-15 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/45944/4/45944.pdf Haron, Normah and Arcot, Jayashree and Bucknall, Martin (2015) Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables. In: Asian Congress on Biotechnology 2015, 15th-19th November 2015, Kuala Lumpur. (Unpublished) |
| spellingShingle | Q Science (General) S Agriculture (General) Haron, Normah Arcot, Jayashree Bucknall, Martin Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables |
| title | Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
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| title_full | Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
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| title_fullStr | Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
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| title_full_unstemmed | Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
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| title_short | Development of LC/MS-MS method for analysis of polyphenols in Malaysian traditional vegetables
|
| title_sort | development of lc/ms-ms method for analysis of polyphenols in malaysian traditional vegetables |
| topic | Q Science (General) S Agriculture (General) |
| url | http://irep.iium.edu.my/45944/ http://irep.iium.edu.my/45944/4/45944.pdf |