Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures
A stoichiometric model was developed for the application of metabolic flux balance analysis (FBA) which consisted of the primary metabolic network of Arabidopsis thaliana (A. thaliana). The basic metabolic model involved 149 metabolites and 178 reactions that represent the main carbon metabolism tow...
| Main Authors: | , |
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2015
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| Online Access: | http://irep.iium.edu.my/44876/ http://irep.iium.edu.my/44876/1/44876.pdf http://irep.iium.edu.my/44876/4/IN-FOO-002.pdf |
| _version_ | 1848782684194603008 |
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| author | Mohamad Puad, Noor Illi Mavituna, Ferda |
| author_facet | Mohamad Puad, Noor Illi Mavituna, Ferda |
| author_sort | Mohamad Puad, Noor Illi |
| building | IIUM Repository |
| collection | Online Access |
| description | A stoichiometric model was developed for the application of metabolic flux balance analysis (FBA) which consisted of the primary metabolic network of Arabidopsis thaliana (A. thaliana). The basic metabolic model involved 149 metabolites and 178 reactions that represent the main carbon metabolism towards the formation of biomass. Assuming pseudo-steady state, mass balances were performed for each metabolite and the resulting equations were solved using linear optimization. The optimal metabolic flux distribution was determined in General Algebraic Modeling System (GAMS) 23.0 software by setting up the objective function as the maximization of biomass formation rate and using the constraints from batch experiments of A. thaliana suspended cell cultures. The results gave the theoretical maximum biological capacity of the cells to grow for a given specific uptake rate of sugar. The model proved to be a good starting point for more extended version of metabolism specifically for secondary metabolite production. Addition or extension of the model is feasible. |
| first_indexed | 2025-11-14T16:09:22Z |
| format | Proceeding Paper |
| id | iium-44876 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:09:22Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-448762016-05-24T00:40:01Z http://irep.iium.edu.my/44876/ Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures Mohamad Puad, Noor Illi Mavituna, Ferda T Technology (General) A stoichiometric model was developed for the application of metabolic flux balance analysis (FBA) which consisted of the primary metabolic network of Arabidopsis thaliana (A. thaliana). The basic metabolic model involved 149 metabolites and 178 reactions that represent the main carbon metabolism towards the formation of biomass. Assuming pseudo-steady state, mass balances were performed for each metabolite and the resulting equations were solved using linear optimization. The optimal metabolic flux distribution was determined in General Algebraic Modeling System (GAMS) 23.0 software by setting up the objective function as the maximization of biomass formation rate and using the constraints from batch experiments of A. thaliana suspended cell cultures. The results gave the theoretical maximum biological capacity of the cells to grow for a given specific uptake rate of sugar. The model proved to be a good starting point for more extended version of metabolism specifically for secondary metabolite production. Addition or extension of the model is feasible. 2015-09-24 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/44876/1/44876.pdf application/pdf en http://irep.iium.edu.my/44876/4/IN-FOO-002.pdf Mohamad Puad, Noor Illi and Mavituna, Ferda (2015) Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures. In: The 22nd Regional Symposium on Chemical Engineering (RSCE 2015), 24th-25th Sept. 2015, Bangkok, Thailand. http://www.rsce2015.org/page/1 |
| spellingShingle | T Technology (General) Mohamad Puad, Noor Illi Mavituna, Ferda Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures |
| title | Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures |
| title_full | Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures |
| title_fullStr | Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures |
| title_full_unstemmed | Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures |
| title_short | Metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures |
| title_sort | metabolic flux balance analysis of the growth of arabidopsis thaliana cell suspension cultures |
| topic | T Technology (General) |
| url | http://irep.iium.edu.my/44876/ http://irep.iium.edu.my/44876/ http://irep.iium.edu.my/44876/1/44876.pdf http://irep.iium.edu.my/44876/4/IN-FOO-002.pdf |