Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice
The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healt...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2015
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| Online Access: | http://irep.iium.edu.my/44455/ http://irep.iium.edu.my/44455/1/44455.pdf |
| _version_ | 1848782609792892928 |
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| author | Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati |
| author_facet | Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati |
| author_sort | Abdul Ghani, Radiah |
| building | IIUM Repository |
| collection | Online Access |
| description | The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. It was found that ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism.
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| first_indexed | 2025-11-14T16:08:11Z |
| format | Proceeding Paper |
| id | iium-44455 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:08:11Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-444552018-01-03T01:26:52Z http://irep.iium.edu.my/44455/ Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati RB Pathology The polyamine biosynthesis is a ubiquitous pathway with a significant role in cell growth , both normal and malignant. As polyamines are crucial in cellular growth and differentiation, they are linked to the development of cancer, with higher polyamine level observed in cancerous cells than in healthy cells. Accordingly, suppressing the polyamine pathway has been found to disrupt tumour development. Chemoprevention is considered a more feasible option in cancer management than chemotherapy, with a focus on natural chemopreventive agent. Pomegranate is known to inhibit several progression of lung cancer, although prior studies on the chemopreventive effect of pomegranate on lung cancer did not explore into polyamine pathway. Hence, this study investigated the effect of pomegranate juice on the polyamine pathway, by focusing on the biosynthesis involving ornithine decarboxylase (ODC), the rate limiting enzyme in the pathway. Quantitative polymerase chain reaction (qPCR) was applied to quantify the changes in ODC gene expression in A549 cells treated with pomegranate. It was found that ODC expression was significantly inhibited compared to untreated cells, with a 48-fold difference. While this finding supports the hypothesis, there is much yet to be elucidated regarding its exact mechanism. 2015 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/44455/1/44455.pdf Abdul Ghani, Radiah and Md Yusof, Najwa Farhah and Nik Abdul Malek, Nik Nurasyikin and A.Talib, Norlelawati (2015) Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice. In: International Conference on Advancement in Science and Technology (iCAST 2015) - ‘Frontiers in Biotechnology’, 10-12 August 2015, Impiana Resort Cherating, Pahang, Malaysia. (Unpublished) |
| spellingShingle | RB Pathology Abdul Ghani, Radiah Md Yusof, Najwa Farhah Nik Abdul Malek, Nik Nurasyikin A.Talib, Norlelawati Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice |
| title | Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice |
| title_full | Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice |
| title_fullStr | Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice |
| title_full_unstemmed | Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice |
| title_short | Ornithine decarboxylase gene expression in human lung carcinoma cells, A549 treated with pomegranate juice |
| title_sort | ornithine decarboxylase gene expression in human lung carcinoma cells, a549 treated with pomegranate juice |
| topic | RB Pathology |
| url | http://irep.iium.edu.my/44455/ http://irep.iium.edu.my/44455/1/44455.pdf |