Comparison of cell disruption method for E. coli BL21-AI expressing recombinant bromelain

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Comparison of cell disruption method for E. coli BL21-AI expressing recombinant bromelain

The often-encountered problem such as protein degradation has driven various methods of cell lysis post fermentation used. In this paper we compare the use of homogenization, sonication, sonication with lysozyme and chemical lysis using B-PER reagent with lysozyme to extract the recombinant bromelai...

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Bibliographic Details
Main Authors:	Mohd Arshad, Zatul Iffah, Amid, Azura, Othman, Mohd Ezza Faiez
Format:	Proceeding Paper
Language:	English
Published:	2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/44445/ http://irep.iium.edu.my/44445/1/iCAST-Zatul.pdf

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