Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA
Cancer is defined as a group of neoplastic diseases broadly characterized by abnormalities of cellular differentiation, maturation and control of growth. The mutation of p53 is one of the most common events found in human cancers. The p53 tumor suppressor protein plays a central role in the cellular...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/4433/ http://irep.iium.edu.my/4433/1/Taher_34.pdf |
| _version_ | 1848776328881373184 |
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| author | Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Abdullah, Zafirah L. |
| author_facet | Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Abdullah, Zafirah L. |
| author_sort | Bakhtiar, M. Taher |
| building | IIUM Repository |
| collection | Online Access |
| description | Cancer is defined as a group of neoplastic diseases broadly characterized by abnormalities of cellular differentiation, maturation and control of growth. The mutation of p53 is one of the most common events found in human cancers. The p53 tumor suppressor protein plays a central role in the cellular response to DNA damage by controlling genes involved in cell cycle arrest and apoptosis. When p53 is lost of its function this may permit the cell to divide without control.
The purpose of the research is to produce human recombinant protein that mimic a specific mutation of the p53 gene by polymerase chain reaction (PCR) based site-directed mutagenesis of wild type p53 plasmid. In the future, the recombinant protein can be use to prevent or to treat the cancer as a part of gene therapy. |
| first_indexed | 2025-11-14T14:28:21Z |
| format | Proceeding Paper |
| id | iium-4433 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:28:21Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-44332014-12-09T01:40:24Z http://irep.iium.edu.my/4433/ Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Abdullah, Zafirah L. RM300 Drugs and their action Cancer is defined as a group of neoplastic diseases broadly characterized by abnormalities of cellular differentiation, maturation and control of growth. The mutation of p53 is one of the most common events found in human cancers. The p53 tumor suppressor protein plays a central role in the cellular response to DNA damage by controlling genes involved in cell cycle arrest and apoptosis. When p53 is lost of its function this may permit the cell to divide without control. The purpose of the research is to produce human recombinant protein that mimic a specific mutation of the p53 gene by polymerase chain reaction (PCR) based site-directed mutagenesis of wild type p53 plasmid. In the future, the recombinant protein can be use to prevent or to treat the cancer as a part of gene therapy. 2010 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/4433/1/Taher_34.pdf Bakhtiar, M. Taher and Arief Ichwan, Solachuddin Jauhari and Abdullah, Zafirah L. (2010) Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA. In: The 21st International Invention, Innovation and Technology Exhibition (ITEX), 14 -16 May 2010, Kuala Lumpur Convention Centre, Malaysia. http://www.itex.com.my/ |
| spellingShingle | RM300 Drugs and their action Bakhtiar, M. Taher Arief Ichwan, Solachuddin Jauhari Abdullah, Zafirah L. Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA |
| title | Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA |
| title_full | Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA |
| title_fullStr | Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA |
| title_full_unstemmed | Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA |
| title_short | Production of recombinant p53 protein by PCR-based site directed mutagenesis of plasmid DNA |
| title_sort | production of recombinant p53 protein by pcr-based site directed mutagenesis of plasmid dna |
| topic | RM300 Drugs and their action |
| url | http://irep.iium.edu.my/4433/ http://irep.iium.edu.my/4433/ http://irep.iium.edu.my/4433/1/Taher_34.pdf |