Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications
Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of microwave radiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of microwave effe...
| Main Authors: | , , , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
Springer Berlin Heidelberg
2010
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/1/Decellularization_of_Living_Tissue_Using_Microwave_Chemical_Process_for_Tissue-Engineered_Scaffold_Applications.pdf http://irep.iium.edu.my/44078/4/44078.pdf |
| _version_ | 1848782550571417600 |
|---|---|
| author | Noor Azmi, Azran Azhim Narita, Y. Muramatsu, K. Morimoto, Y. Tanaka, Masato |
| author_facet | Noor Azmi, Azran Azhim Narita, Y. Muramatsu, K. Morimoto, Y. Tanaka, Masato |
| author_sort | Noor Azmi, Azran Azhim |
| building | IIUM Repository |
| collection | Online Access |
| description | Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of microwave radiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of microwave effects on decellularization of soft and cartilage tissues. The targeted tissues were decellularized using 2450 MHz of microwave radiation in pulse-circulated 0.9% sodium chloride constituent of 1% sodium deoxycholate solution. To evaluate tissue integrity and cell removal, we used hematoxylin-eosin staining and scanning electron microscope. To investigate the effect of microwave power on targeted sample, we evaluate the radiated microwave distribution in microwave oven using thermograpic camera. Histological results suggest that microwave power influenced the decellularization of samples. It is significantly decellularized, when compared with the other preparation methods. Due to distributions of microwave radiation in the oven are non-uniform, the efficiency of cell removal can be improved by fitting the sample at uniform distribution and higher radiation spot of microwave. In conclusion, combination of microwave radiation and chemically detergent process has potential to decellularize various tissues, even tendon tissue. However, optimization of electromagnetic field in the oven is needed to perform in the further study to improve the decellularization efficiency. |
| first_indexed | 2025-11-14T16:07:15Z |
| format | Proceeding Paper |
| id | iium-44078 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:07:15Z |
| publishDate | 2010 |
| publisher | Springer Berlin Heidelberg |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-440782015-08-04T07:48:27Z http://irep.iium.edu.my/44078/ Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications Noor Azmi, Azran Azhim Narita, Y. Muramatsu, K. Morimoto, Y. Tanaka, Masato TP248.13 Biotechnology Scaffolds play a key role in the process of regeneration and morphogenesis of tissue or organ. We have studied a novel application of microwave radiation to prepare decellularized tissue for tissue-engineered scaffolds. The aim of the present study is to investigate the possibility of microwave effects on decellularization of soft and cartilage tissues. The targeted tissues were decellularized using 2450 MHz of microwave radiation in pulse-circulated 0.9% sodium chloride constituent of 1% sodium deoxycholate solution. To evaluate tissue integrity and cell removal, we used hematoxylin-eosin staining and scanning electron microscope. To investigate the effect of microwave power on targeted sample, we evaluate the radiated microwave distribution in microwave oven using thermograpic camera. Histological results suggest that microwave power influenced the decellularization of samples. It is significantly decellularized, when compared with the other preparation methods. Due to distributions of microwave radiation in the oven are non-uniform, the efficiency of cell removal can be improved by fitting the sample at uniform distribution and higher radiation spot of microwave. In conclusion, combination of microwave radiation and chemically detergent process has potential to decellularize various tissues, even tendon tissue. However, optimization of electromagnetic field in the oven is needed to perform in the further study to improve the decellularization efficiency. Springer Berlin Heidelberg 2010 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/44078/1/Decellularization_of_Living_Tissue_Using_Microwave_Chemical_Process_for_Tissue-Engineered_Scaffold_Applications.pdf application/pdf en http://irep.iium.edu.my/44078/4/44078.pdf Noor Azmi, Azran Azhim and Narita, Y. and Muramatsu, K. and Morimoto, Y. and Tanaka, Masato (2010) Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications. In: 6th World Congress of Biomechanics (WCB 2010), 1-6 August 2010, Singapore. http://link.springer.com/chapter/10.1007%2F978-3-642-14515-5_238 doi:10.1007/978-3-642-14515-5_238 |
| spellingShingle | TP248.13 Biotechnology Noor Azmi, Azran Azhim Narita, Y. Muramatsu, K. Morimoto, Y. Tanaka, Masato Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications |
| title | Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications |
| title_full | Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications |
| title_fullStr | Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications |
| title_full_unstemmed | Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications |
| title_short | Decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications |
| title_sort | decellularization of living tissue using microwave chemical process for tissue-engineered scaffold applications |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/ http://irep.iium.edu.my/44078/1/Decellularization_of_Living_Tissue_Using_Microwave_Chemical_Process_for_Tissue-Engineered_Scaffold_Applications.pdf http://irep.iium.edu.my/44078/4/44078.pdf |