Development of a module for cell culture based on a decellularized vascular tissue scaffold
Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a de...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English English |
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J-STAGE
2011
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| Online Access: | http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/1/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf http://irep.iium.edu.my/44074/4/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf |
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| author | Ataru , MURAHARA Akimitsu , WAKIDA Yu , MIYAKE Shoichiro , TATEISHI Takahiro , HIRAYAMA Noor Azmi, Azran Azhim Kazuaki , MURAMATSU Masato , TANAKA Osamu , ENDO Kozo , MIYAZAKI Hirotaka , MATSUO Nariyoshi , SHINOMIYA Yuji , MORIMOTO |
| author_facet | Ataru , MURAHARA Akimitsu , WAKIDA Yu , MIYAKE Shoichiro , TATEISHI Takahiro , HIRAYAMA Noor Azmi, Azran Azhim Kazuaki , MURAMATSU Masato , TANAKA Osamu , ENDO Kozo , MIYAZAKI Hirotaka , MATSUO Nariyoshi , SHINOMIYA Yuji , MORIMOTO |
| author_sort | Ataru , MURAHARA |
| building | IIUM Repository |
| collection | Online Access |
| description | Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a detergent at 300 rpm. In addition, we developed a ring-clamp for fixation of decellularized tissue and created a scaffold module for cell culture by the combination of the ring-clamp and decellularized tissue. The module can be placed in a well of a commercially available 24-well plate. Smooth muscle cells that were seeded on a scaffold module consisting of a decellularized vessel and the ring-clamp showed proliferation with a spindle shape similar to that of vascular smooth muscle cells in the biological body. Vascular endothelial cells that adhered to the scaffold module showed enhanced susceptibility to histamine-induced Ca2+ influx. |
| first_indexed | 2025-11-14T16:07:14Z |
| format | Article |
| id | iium-44074 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:07:14Z |
| publishDate | 2011 |
| publisher | J-STAGE |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-440742015-08-11T00:42:07Z http://irep.iium.edu.my/44074/ Development of a module for cell culture based on a decellularized vascular tissue scaffold Ataru , MURAHARA Akimitsu , WAKIDA Yu , MIYAKE Shoichiro , TATEISHI Takahiro , HIRAYAMA Noor Azmi, Azran Azhim Kazuaki , MURAMATSU Masato , TANAKA Osamu , ENDO Kozo , MIYAZAKI Hirotaka , MATSUO Nariyoshi , SHINOMIYA Yuji , MORIMOTO TP248.13 Biotechnology Decellularized tissues and organs have been intensively studied in order to use them as a scaffold for proliferation of recipient cells in tissue engineering/regenerative medicine applications. We established a fast and effective method for tissue decellularization by way of shaking a tissue in a detergent at 300 rpm. In addition, we developed a ring-clamp for fixation of decellularized tissue and created a scaffold module for cell culture by the combination of the ring-clamp and decellularized tissue. The module can be placed in a well of a commercially available 24-well plate. Smooth muscle cells that were seeded on a scaffold module consisting of a decellularized vessel and the ring-clamp showed proliferation with a spindle shape similar to that of vascular smooth muscle cells in the biological body. Vascular endothelial cells that adhered to the scaffold module showed enhanced susceptibility to histamine-induced Ca2+ influx. J-STAGE 2011 Article PeerReviewed application/pdf en http://irep.iium.edu.my/44074/1/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf application/pdf en http://irep.iium.edu.my/44074/4/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf Ataru , MURAHARA and Akimitsu , WAKIDA and Yu , MIYAKE and Shoichiro , TATEISHI and Takahiro , HIRAYAMA and Noor Azmi, Azran Azhim and Kazuaki , MURAMATSU and Masato , TANAKA and Osamu , ENDO and Kozo , MIYAZAKI and Hirotaka , MATSUO and Nariyoshi , SHINOMIYA and Yuji , MORIMOTO (2011) Development of a module for cell culture based on a decellularized vascular tissue scaffold. Transactions of Japanese Society for Medical and Biological Engineering , 49 (3). pp. 508-515. ISSN 1881-4379 https://www.jstage.jst.go.jp/article/jsmbe/49/3/49_3_508/_article http://doi.org/10.11239/jsmbe.49.508 |
| spellingShingle | TP248.13 Biotechnology Ataru , MURAHARA Akimitsu , WAKIDA Yu , MIYAKE Shoichiro , TATEISHI Takahiro , HIRAYAMA Noor Azmi, Azran Azhim Kazuaki , MURAMATSU Masato , TANAKA Osamu , ENDO Kozo , MIYAZAKI Hirotaka , MATSUO Nariyoshi , SHINOMIYA Yuji , MORIMOTO Development of a module for cell culture based on a decellularized vascular tissue scaffold |
| title | Development of a module for cell culture based on a decellularized vascular tissue scaffold |
| title_full | Development of a module for cell culture based on a decellularized vascular tissue scaffold |
| title_fullStr | Development of a module for cell culture based on a decellularized vascular tissue scaffold |
| title_full_unstemmed | Development of a module for cell culture based on a decellularized vascular tissue scaffold |
| title_short | Development of a module for cell culture based on a decellularized vascular tissue scaffold |
| title_sort | development of a module for cell culture based on a decellularized vascular tissue scaffold |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/ http://irep.iium.edu.my/44074/1/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf http://irep.iium.edu.my/44074/4/Development_of_a_Module_for_Cell_Culture_Based_on_a_Decellularized_Vascular_Tissue_Scaffold.pdf |