In vivo study of implanted aortic scaffolds decelluraized by sonication treatment
Sonication treatment is used in the preparation of scaffold that elicitsminimal immune response after implantation. The aim of this study is to investigate the immunologic responseof sonicatedly decellularizedaortic scaffoldsafter 7 and 35 days of subcutaneousimplantation. In this study, aorta sampl...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2013
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| Online Access: | http://irep.iium.edu.my/44038/ http://irep.iium.edu.my/44038/4/proceeding_book.pdf http://irep.iium.edu.my/44038/1/IN_VIVO_STUDY_OF_IMPLANTED_AORTIC_SCAFFOLDS_DECELLULARIZED_BY.pdf |
| _version_ | 1848782542740652032 |
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| author | Noor Azmi, Azran Azhim N. , Syazwani Y. , Morimoto K.S. , Furukawa T. , Ushida |
| author_facet | Noor Azmi, Azran Azhim N. , Syazwani Y. , Morimoto K.S. , Furukawa T. , Ushida |
| author_sort | Noor Azmi, Azran Azhim |
| building | IIUM Repository |
| collection | Online Access |
| description | Sonication treatment is used in the preparation of scaffold that elicitsminimal immune response after implantation. The aim of this study is to investigate the immunologic responseof sonicatedly decellularizedaortic scaffoldsafter 7 and 35 days of subcutaneousimplantation. In this study, aorta samples weresonicatedly decellularized in0.1% and 2% SDS detergentfor 10 hours. It wasfollowed by washing process withPBS solution for 5 days. The treated samples were then evaluated histologically by Hematoxylin Eosin (HE) stainingand DNA quantification. The samples were subcutaneously implanted on the lower right thoracic cavity ofWistar rats. The implants were explanted and evaluated by HE staining. From the histological results, the implanted samples after sonicatedly decellularized with 2% SDS shows minimal immune response,compared to that decellularized with 0.1% SDS detergents. Hence, sonicatedly decellularized scaffolds treated with 2% SDS showedgreat potential as future biomedical implants. |
| first_indexed | 2025-11-14T16:07:07Z |
| format | Proceeding Paper |
| id | iium-44038 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:07:07Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-440382015-08-19T03:14:39Z http://irep.iium.edu.my/44038/ In vivo study of implanted aortic scaffolds decelluraized by sonication treatment Noor Azmi, Azran Azhim N. , Syazwani Y. , Morimoto K.S. , Furukawa T. , Ushida TP248.13 Biotechnology Sonication treatment is used in the preparation of scaffold that elicitsminimal immune response after implantation. The aim of this study is to investigate the immunologic responseof sonicatedly decellularizedaortic scaffoldsafter 7 and 35 days of subcutaneousimplantation. In this study, aorta samples weresonicatedly decellularized in0.1% and 2% SDS detergentfor 10 hours. It wasfollowed by washing process withPBS solution for 5 days. The treated samples were then evaluated histologically by Hematoxylin Eosin (HE) stainingand DNA quantification. The samples were subcutaneously implanted on the lower right thoracic cavity ofWistar rats. The implants were explanted and evaluated by HE staining. From the histological results, the implanted samples after sonicatedly decellularized with 2% SDS shows minimal immune response,compared to that decellularized with 0.1% SDS detergents. Hence, sonicatedly decellularized scaffolds treated with 2% SDS showedgreat potential as future biomedical implants. 2013 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/44038/4/proceeding_book.pdf application/pdf en http://irep.iium.edu.my/44038/1/IN_VIVO_STUDY_OF_IMPLANTED_AORTIC_SCAFFOLDS_DECELLULARIZED_BY.pdf Noor Azmi, Azran Azhim and N. , Syazwani and Y. , Morimoto and K.S. , Furukawa and T. , Ushida (2013) In vivo study of implanted aortic scaffolds decelluraized by sonication treatment. In: The MJIIT-JUC Joint International Symposium 2013 (MJJIS2013), 6-8 November 2013, Tokai University. (Unpublished) |
| spellingShingle | TP248.13 Biotechnology Noor Azmi, Azran Azhim N. , Syazwani Y. , Morimoto K.S. , Furukawa T. , Ushida In vivo study of implanted aortic scaffolds decelluraized by sonication treatment |
| title | In vivo study of implanted aortic scaffolds decelluraized by sonication treatment |
| title_full | In vivo study of implanted aortic scaffolds decelluraized by sonication treatment |
| title_fullStr | In vivo study of implanted aortic scaffolds decelluraized by sonication treatment |
| title_full_unstemmed | In vivo study of implanted aortic scaffolds decelluraized by sonication treatment |
| title_short | In vivo study of implanted aortic scaffolds decelluraized by sonication treatment |
| title_sort | in vivo study of implanted aortic scaffolds decelluraized by sonication treatment |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/44038/ http://irep.iium.edu.my/44038/4/proceeding_book.pdf http://irep.iium.edu.my/44038/1/IN_VIVO_STUDY_OF_IMPLANTED_AORTIC_SCAFFOLDS_DECELLULARIZED_BY.pdf |