Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RN...
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| Format: | Article |
| Language: | English English |
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Piracicaba Dental School, Brazil
2015
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| Online Access: | http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/1/2015-BJOS-_Expression_analysis_of_Notch_signaling_pathway_molecules_in_SHED.pdf http://irep.iium.edu.my/43699/4/43699_Expression%20analysis%20of%20notch%20signaling%20pathway_SCOPUS.pdf |
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| author | Mohd Taha, Siti Aisyah Koh Su Ling, Joanne Azmi, Nur Izyan Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar@Makhtar, Khairani Idah |
| author_facet | Mohd Taha, Siti Aisyah Koh Su Ling, Joanne Azmi, Nur Izyan Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar@Makhtar, Khairani Idah |
| author_sort | Mohd Taha, Siti Aisyah |
| building | IIUM Repository |
| collection | Online Access |
| description | Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells
from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely,
keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on
day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR
using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2
and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2%
agarose gel and stained with SYBR green. Results: Notch-1 was highly expressed in SHED
cultured in KGM and showed increase in density as the days progressed, while Jagged-1
showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed
by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog
showed expression only on day 3 and gradually increased in expression on day 7.
Conclusions: Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2,
and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in
the differentiation into epithelial-like cells in human dental pulp tissues.
Keywords: receptors, notch; gene expression; stem cells; tooth, deciduous; culture media. |
| first_indexed | 2025-11-14T16:06:17Z |
| format | Article |
| id | iium-43699 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:06:17Z |
| publishDate | 2015 |
| publisher | Piracicaba Dental School, Brazil |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-436992022-10-25T02:50:27Z http://irep.iium.edu.my/43699/ Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium Mohd Taha, Siti Aisyah Koh Su Ling, Joanne Azmi, Nur Izyan Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar@Makhtar, Khairani Idah RK Dentistry Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). Methods: RNA was extracted from SHED harvested on day 1, 3 and 7. RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). PCR products were electrophoresed on a 2% agarose gel and stained with SYBR green. Results: Notch-1 was highly expressed in SHED cultured in KGM and showed increase in density as the days progressed, while Jagged-1 showed a decrease. Jagged-2 on the other hand, showed a slight increase on day 3 followed by a decrease on day 7. However, Hes-1 was not expressed in SHED cultured in KGM. Nanog showed expression only on day 3 and gradually increased in expression on day 7. Conclusions: Notch signaling pathway associated molecules; Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. Keywords: receptors, notch; gene expression; stem cells; tooth, deciduous; culture media. Piracicaba Dental School, Brazil 2015-06-09 Article PeerReviewed application/pdf en http://irep.iium.edu.my/43699/1/2015-BJOS-_Expression_analysis_of_Notch_signaling_pathway_molecules_in_SHED.pdf application/pdf en http://irep.iium.edu.my/43699/4/43699_Expression%20analysis%20of%20notch%20signaling%20pathway_SCOPUS.pdf Mohd Taha, Siti Aisyah and Koh Su Ling, Joanne and Azmi, Nur Izyan and Thirumulu Ponnuraj, Kannan and Ahmad, Azlina and Mokhtar@Makhtar, Khairani Idah (2015) Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium. Brazilian Journal of Oral Sciences, 14 (2). pp. 135-140. ISSN 1677-3225 http://www.scielo.br/ |
| spellingShingle | RK Dentistry Mohd Taha, Siti Aisyah Koh Su Ling, Joanne Azmi, Nur Izyan Thirumulu Ponnuraj, Kannan Ahmad, Azlina Mokhtar@Makhtar, Khairani Idah Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium |
| title | Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium |
| title_full | Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium |
| title_fullStr | Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium |
| title_full_unstemmed | Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium |
| title_short | Expression analysis of notch signaling pathway molecules in SHED cultured in keratinocyte growth medium |
| title_sort | expression analysis of notch signaling pathway molecules in shed cultured in keratinocyte growth medium |
| topic | RK Dentistry |
| url | http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/ http://irep.iium.edu.my/43699/1/2015-BJOS-_Expression_analysis_of_Notch_signaling_pathway_molecules_in_SHED.pdf http://irep.iium.edu.my/43699/4/43699_Expression%20analysis%20of%20notch%20signaling%20pathway_SCOPUS.pdf |