Case study: recombinant Bromelain cloning, characterization and upstream processes
This chapter presents the recombinant stem bromelain cloning procedure followed by its characterization and finally upstream processing. Most of the procedures had patents filed in Malaysia, Europe and the United State of America with application numbers PI 20095434, 10015711.4, and 12968766, respe...
| Main Authors: | Amid, Azura, Ismail, Nurul Azira, Jamaluddin, Mohd Jamil Aizat |
|---|---|
| Format: | Book Chapter |
| Language: | English English |
| Published: |
Springer International Publishing
2015
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/43383/ http://irep.iium.edu.my/43383/1/Case_study-recombinant_bromelain_cloning-chapter_11.pdf http://irep.iium.edu.my/43383/3/Recombinant_Enzymes_TOC.pdf |
Similar Items
Case Study: Recombinant Bromelain Selection
by: Amid, Azura, et al.
Published: (2015)
by: Amid, Azura, et al.
Published: (2015)
Functional characterization of recombinant bromelain
by: Amid, Azura, et al.
Published: (2015)
by: Amid, Azura, et al.
Published: (2015)
Effects of operating conditions in spray drying of recombinant bromelain
by: Amid, Azura, et al.
Published: (2014)
by: Amid, Azura, et al.
Published: (2014)
Case Study: Recombinant Bromelain
Downstream Processing
by: Amid, Azura, et al.
Published: (2015)
by: Amid, Azura, et al.
Published: (2015)
Observation on recombinant bromelain activity before and after purification
by: Ismail, Nurul Azira, et al.
Published: (2010)
by: Ismail, Nurul Azira, et al.
Published: (2010)
Process optimization on cell disruption of recombinant bromelain expressed in E. coli BL21-AI using ultrasonication
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2013)
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2013)
Effects of process parameters on spray drying of recombinant bromelain
by: Muntari, Bala, et al.
Published: (2013)
by: Muntari, Bala, et al.
Published: (2013)
Bromelain enzyme assay using Casen as substrate
by: Amid, Azura, et al.
Published: (2016)
by: Amid, Azura, et al.
Published: (2016)
Optimization on cell disruption of E. coli BL21-AI
expressing recombinant bromelain
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2014)
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2014)
Kinetic studies on recombinant stem bromelain
by: Bala, Muntari, et al.
Published: (2013)
by: Bala, Muntari, et al.
Published: (2013)
Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2014)
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2014)
Molecular cloning sequencing purification of stem bromelain from Ananas comosus
by: Amid, Azura, et al.
Published: (2010)
by: Amid, Azura, et al.
Published: (2010)
Recombinant-enzyme fermentation
by: Amid, Azura, et al.
Published: (2015)
by: Amid, Azura, et al.
Published: (2015)
Determination of optimal range of post-induction temperature for production of soluble recombinant bromelain in Escherichia coli using one-factor-at-a-time (OFAT) approach
by: Amid, Azura, et al.
Published: (2011)
by: Amid, Azura, et al.
Published: (2011)
Recombinant Enzyme: Cloning and Expression
by: Amid, Azura, et al.
Published: (2015)
by: Amid, Azura, et al.
Published: (2015)
Gene expression analysis in MCF-7 breast cancer cells
treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)
by: Fouz, Nour, et al.
Published: (2014)
Pathway analysis of genes affected in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)
by: Fouz, Nour, et al.
Published: (2014)
Comparison of cell disruption method for E. coli BL21-AI expressing recombinant bromelain
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)
Screening of autoinduction medium composition for optimial biomass production of E.coli expressing stem bromelain
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2013)
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2013)
Comparison of different cell disruption methods and cell extractant buffers for recombinant bromelain expressed in E.coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)
Production of intracellular recombinant bromelain by Escherichia coli BL21-AI using shake flask and stirred tank bioreactor
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2017)
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2017)
Heterologous expression of Bromelain in Escherichia coli
by: M., Bala, et al.
Published: (2011)
by: M., Bala, et al.
Published: (2011)
Comparison of purification methods to purify recombinant bromelain from Escherichia coli BL21-A1 = Perbandingan kaedah penulenan rekombinan bromelain daripada Escherichia coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2017)
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2017)
Up-scaling cream cheese production by 100 l per day cow milk using bromelain as rennet alternative
by: Amid, Azura
Published: (2019)
by: Amid, Azura
Published: (2019)
Bromelain: an overview of industrial application
and purification strategies
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2014)
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2014)
Economic and environmental evaluation
of recombinant enzyme production
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2015)
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2015)
Introduction to recombinant enzyme pre-commecialization
by: Amid, Azura
Published: (2015)
by: Amid, Azura
Published: (2015)
Effect of commercial bromelain on viability of MCF-7 human breast cancer cells
by: Yahaya, Hanan, et al.
Published: (2013)
by: Yahaya, Hanan, et al.
Published: (2013)
On-column refolding of recombinant fungal endoglucanase I
by: Aizat Jamaluddin, Mohd Jamil, et al.
Published: (2011)
by: Aizat Jamaluddin, Mohd Jamil, et al.
Published: (2011)
Optimization of cultivation conditions to enhance the
expression of recombinant VDAC2 from gallus gallus
by: Ahmad Hassan, Nurhidayah, et al.
Published: (2014)
by: Ahmad Hassan, Nurhidayah, et al.
Published: (2014)
Common Laboratory Procedure
by: Amid, Azura, et al.
Published: (2015)
by: Amid, Azura, et al.
Published: (2015)
Optimization of cultivation culture conditions in E. coli to enhance the expression of recombinant VDAC2 from Gallus gallus
by: Ahmad Hassan, Nurhidayah, et al.
Published: (2013)
by: Ahmad Hassan, Nurhidayah, et al.
Published: (2013)
Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2
by: Pang, Wei Cheng, et al.
Published: (2020)
by: Pang, Wei Cheng, et al.
Published: (2020)
On-column refolding of recombinant fungal endoglucanase
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2012)
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2012)
Optimization of the elicitation process on chrysanthemum indicum cell suspension culture producing xanthine oxidase inhibitor
by: Amid, Azura, et al.
Published: (2009)
by: Amid, Azura, et al.
Published: (2009)
Optimized preparation and characterization of CLEA-lipase from cocoa pod husk
by: Khanahmadi, Soofia, et al.
Published: (2014)
by: Khanahmadi, Soofia, et al.
Published: (2014)
Purification of recombinant protein for industrial use
by: Yusof, Faridah
Published: (2015)
by: Yusof, Faridah
Published: (2015)
Optimization of extraction process parameters for anti-cancer agent from Solanum Lycopersicum
by: Amid, Azura, et al.
Published: (2011)
by: Amid, Azura, et al.
Published: (2011)
Optimization of the extraction process parameter for kenaf seeds oil to obtain high oil yield
by: Amid, Azura, et al.
Published: (2011)
by: Amid, Azura, et al.
Published: (2011)
Optimization of the extraction process parameter to obtain highest anti-cancer activity from kenaf seeds
by: Amid, Azura, et al.
Published: (2011)
by: Amid, Azura, et al.
Published: (2011)
Similar Items
-
Case Study: Recombinant Bromelain Selection
by: Amid, Azura, et al.
Published: (2015) -
Functional characterization of recombinant bromelain
by: Amid, Azura, et al.
Published: (2015) -
Effects of operating conditions in spray drying of recombinant bromelain
by: Amid, Azura, et al.
Published: (2014) -
Case Study: Recombinant Bromelain
Downstream Processing
by: Amid, Azura, et al.
Published: (2015) -
Observation on recombinant bromelain activity before and after purification
by: Ismail, Nurul Azira, et al.
Published: (2010)