Recombinant Enzyme: Cloning and Expression

QR Code

Recombinant Enzyme: Cloning and Expression

This chapter will discuss the general strategies of cloning recombinant enzymes from identifying a suitable DNA template to choosing the correct host expression system. However, there is no standard operating procedure for cloning a recombinant enzyme. The best strategies should be based on the rese...

Full description

Bibliographic Details
Main Authors:	Amid, Azura, Hassan, Norhidayah
Format:	Book Chapter
Language:	English
Published:	Springer International Publishing 2015
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/43377/ http://irep.iium.edu.my/43377/7/Recombinant_enzyme_cloning_and_expression-chapter_2-completed.pdf

Similar Items

Introduction to recombinant enzyme pre-commecialization
by: Amid, Azura
Published: (2015)

Recombinant-enzyme fermentation
by: Amid, Azura, et al.
Published: (2015)

Case study: recombinant Bromelain cloning, characterization and upstream processes
by: Amid, Azura, et al.
Published: (2015)

Economic and environmental evaluation of recombinant enzyme production
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2015)

Optimization of cultivation conditions to enhance the expression of recombinant VDAC2 from gallus gallus
by: Ahmad Hassan, Nurhidayah, et al.
Published: (2014)

Optimization of cultivation culture conditions in E. coli to enhance the expression of recombinant VDAC2 from Gallus gallus
by: Ahmad Hassan, Nurhidayah, et al.
Published: (2013)

Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Comparison of cell disruption method for E. coli BL21-AI expressing recombinant bromelain
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)

Optimization on cell disruption of E. coli BL21-AI expressing recombinant bromelain
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2014)

Recombinant enzymes—from basic science to commercialization
Published: (2015)

Functional characterization of recombinant bromelain
by: Amid, Azura, et al.
Published: (2015)

Molecular cloning sequencing purification of stem bromelain from Ananas comosus
by: Amid, Azura, et al.
Published: (2010)

Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2014)

Observation on recombinant bromelain activity before and after purification
by: Ismail, Nurul Azira, et al.
Published: (2010)

Bioprocessing of recombinant E.Coli producing Glucuronidase Enzymes
by: Mel, Maizirwan
Published: (2012)

Process optimization on cell disruption of recombinant bromelain expressed in E. coli BL21-AI using ultrasonication
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2013)

Comparison of different cell disruption methods and cell extractant buffers for recombinant bromelain expressed in E.coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)

Case Study: Recombinant Bromelain Selection
by: Amid, Azura, et al.
Published: (2015)

Bromelain enzyme assay using Casen as substrate
by: Amid, Azura, et al.
Published: (2016)

Case Study: Recombinant Bromelain Downstream Processing
by: Amid, Azura, et al.
Published: (2015)

Kinetic studies on recombinant stem bromelain
by: Bala, Muntari, et al.
Published: (2013)

Effects of operating conditions in spray drying of recombinant bromelain
by: Amid, Azura, et al.
Published: (2014)

Effects of process parameters on spray drying of recombinant bromelain
by: Muntari, Bala, et al.
Published: (2013)

Determination of optimal range of post-induction temperature for production of soluble recombinant bromelain in Escherichia coli using one-factor-at-a-time (OFAT) approach
by: Amid, Azura, et al.
Published: (2011)

Pathway analysis of genes affected in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Isolation of thermotolerant bacteria producing fibrinolytic enzyme
by: Ab Shukor, Nurul Aqilah, et al.
Published: (2015)

Isolation of thermotolerant bacteria producing fibrinolytic enzyme
by: Ab. Shukor , Nurul Aqilah, et al.
Published: (2015)

Development of new media formulation for the improvement of recombinant protein expression in E.Coli
by: Mohd. Salleh, Hamzah, et al.
Published: (2012)

Expression of a Fusarium oxysporum endoglucanase in kluyveromyces lactis
by: Moukit, Farid, et al.
Published: (2011)

Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed
by: Hammed, Ademola Monsur, et al.
Published: (2017)

Heterologous expression of Bromelain in Escherichia coli
by: M., Bala, et al.
Published: (2011)

Design and development of a lab scale bioreactor for heat inducible enzyme expression system
by: Adam, Daud, et al.
Published: (2011)

Screening of autoinduction medium composition for optimial biomass production of E.coli expressing stem bromelain
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2013)

Immobilization of enzymes
by: Yusof, Faridah
Published: (2011)

Purification of recombinant protein for industrial use
by: Yusof, Faridah
Published: (2015)

Multi-step enzyme reaction in an Immobilized Enzyme Microreactor (IEMR)
by: Abdul Halim, Amanatuzzakiah, et al.
Published: (2016)

Up-scaling cream cheese production by 100 l per day cow milk using bromelain as rennet alternative
by: Amid, Azura
Published: (2019)

On-column refolding of recombinant fungal endoglucanase I
by: Aizat Jamaluddin, Mohd Jamil, et al.
Published: (2011)

Comparison of purification methods to purify recombinant bromelain from Escherichia coli BL21-A1 = Perbandingan kaedah penulenan rekombinan bromelain daripada Escherichia coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2017)

Factors affecting enzyme assays
by: Mohd. Salleh, Hamzah
Published: (2011)