Semen antioxidant study in male patients at East Coast Malaysia

Reactive oxygen species (ROS) is believed to be one of the factors that cause male infertility. Antioxidants in the seminal plasma plays important role in protecting sperm from ROS. The purpose of this study is to determine the antioxidants level in semen sample in male patients without antioxidant...

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Main Authors: Abdul Wahab, Azantee Yazmie, Ramli, Roszaman, Abd Rahman, S., Md Isa, Muhammad Lokman, Mohd Yatim, Hayati, Mohd Bustaman, Siti Khadijah, M Zulkifli, S N
Format: Proceeding Paper
Language:English
English
Published: 2014
Subjects:
Online Access:http://irep.iium.edu.my/42700/
http://irep.iium.edu.my/42700/1/Semen_antioxidant_Study_In_Male_Patients_At_East_Coast_Malaysia.pdf
http://irep.iium.edu.my/42700/2/ASPIRE-2014_Final-Programme-and-Astract-Book.pdf
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author Abdul Wahab, Azantee Yazmie
Ramli, Roszaman
Abd Rahman, S.
Md Isa, Muhammad Lokman
Mohd Yatim, Hayati
Mohd Bustaman, Siti Khadijah
M Zulkifli, S N
author_facet Abdul Wahab, Azantee Yazmie
Ramli, Roszaman
Abd Rahman, S.
Md Isa, Muhammad Lokman
Mohd Yatim, Hayati
Mohd Bustaman, Siti Khadijah
M Zulkifli, S N
author_sort Abdul Wahab, Azantee Yazmie
building IIUM Repository
collection Online Access
description Reactive oxygen species (ROS) is believed to be one of the factors that cause male infertility. Antioxidants in the seminal plasma plays important role in protecting sperm from ROS. The purpose of this study is to determine the antioxidants level in semen sample in male patients without antioxidant treatment at IIUM Fertility Centre, Malaysia. A total of 43 semen samples were taken and seminal fluid analysis (SFA) test was performed according to WHO 1999 guideline. Patients were 234 divided into two groups; normozoospermia and oligozoospermia. Semen samples were then stored in liquid nitrogen at 196 ?C using standard preservation technique. The samples were thawed and centrifuged to separate the seminal plasma and the sperm. The antioxidant levels of Vitamin C and Vitamin E in seminal plasma were measured using the spectrophotometer. Descriptive statistic was done for SFA to summarize the result of sperm parameters and antioxidant levels. It is shown in this study that the level of antioxidants, both Vitamin E and Vitamin C, are higher in the normozoospermia samples compared to the oligozoospermia samples (p<0.05). There is a positive correlation between the sperm concentration and the level of antioxidants.
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format Proceeding Paper
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institution International Islamic University Malaysia
institution_category Local University
language English
English
last_indexed 2025-11-14T16:03:29Z
publishDate 2014
recordtype eprints
repository_type Digital Repository
spelling iium-427002019-11-21T08:54:59Z http://irep.iium.edu.my/42700/ Semen antioxidant study in male patients at East Coast Malaysia Abdul Wahab, Azantee Yazmie Ramli, Roszaman Abd Rahman, S. Md Isa, Muhammad Lokman Mohd Yatim, Hayati Mohd Bustaman, Siti Khadijah M Zulkifli, S N RG Gynecology and obstetrics Reactive oxygen species (ROS) is believed to be one of the factors that cause male infertility. Antioxidants in the seminal plasma plays important role in protecting sperm from ROS. The purpose of this study is to determine the antioxidants level in semen sample in male patients without antioxidant treatment at IIUM Fertility Centre, Malaysia. A total of 43 semen samples were taken and seminal fluid analysis (SFA) test was performed according to WHO 1999 guideline. Patients were 234 divided into two groups; normozoospermia and oligozoospermia. Semen samples were then stored in liquid nitrogen at 196 ?C using standard preservation technique. The samples were thawed and centrifuged to separate the seminal plasma and the sperm. The antioxidant levels of Vitamin C and Vitamin E in seminal plasma were measured using the spectrophotometer. Descriptive statistic was done for SFA to summarize the result of sperm parameters and antioxidant levels. It is shown in this study that the level of antioxidants, both Vitamin E and Vitamin C, are higher in the normozoospermia samples compared to the oligozoospermia samples (p<0.05). There is a positive correlation between the sperm concentration and the level of antioxidants. 2014 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/42700/1/Semen_antioxidant_Study_In_Male_Patients_At_East_Coast_Malaysia.pdf application/pdf en http://irep.iium.edu.my/42700/2/ASPIRE-2014_Final-Programme-and-Astract-Book.pdf Abdul Wahab, Azantee Yazmie and Ramli, Roszaman and Abd Rahman, S. and Md Isa, Muhammad Lokman and Mohd Yatim, Hayati and Mohd Bustaman, Siti Khadijah and M Zulkifli, S N (2014) Semen antioxidant study in male patients at East Coast Malaysia. In: 5th Congress of the Asia Pacific Initiative on Reproduction, ASPIRE 2014, 4-6 Apr 2014, Brisbane, Australia. http://aspirecongress.org/
spellingShingle RG Gynecology and obstetrics
Abdul Wahab, Azantee Yazmie
Ramli, Roszaman
Abd Rahman, S.
Md Isa, Muhammad Lokman
Mohd Yatim, Hayati
Mohd Bustaman, Siti Khadijah
M Zulkifli, S N
Semen antioxidant study in male patients at East Coast Malaysia
title Semen antioxidant study in male patients at East Coast Malaysia
title_full Semen antioxidant study in male patients at East Coast Malaysia
title_fullStr Semen antioxidant study in male patients at East Coast Malaysia
title_full_unstemmed Semen antioxidant study in male patients at East Coast Malaysia
title_short Semen antioxidant study in male patients at East Coast Malaysia
title_sort semen antioxidant study in male patients at east coast malaysia
topic RG Gynecology and obstetrics
url http://irep.iium.edu.my/42700/
http://irep.iium.edu.my/42700/
http://irep.iium.edu.my/42700/1/Semen_antioxidant_Study_In_Male_Patients_At_East_Coast_Malaysia.pdf
http://irep.iium.edu.my/42700/2/ASPIRE-2014_Final-Programme-and-Astract-Book.pdf