Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions
Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of alpha-mangostin, a major xanth...
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| Format: | Article |
| Language: | English English English |
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Hindawi Publishing Corporation
2015
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| Online Access: | http://irep.iium.edu.my/42492/ http://irep.iium.edu.my/42492/1/Alpha_mangostin_ECAM.pdf http://irep.iium.edu.my/42492/4/42492_Alpha-mangostin%20improves%20glucose%20uptake%20and%20inhibits_Scopus.pdf http://irep.iium.edu.my/42492/5/42492_Alpha-mangostin%20improves%20glucose%20uptake%20and%20inhibits_WOS.pdf |
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| author | Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Tengku Zakaria, Tengku Muhamad Faris Syafiq Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Ahmed, Qamar Uddin Zakaria, Zainul Amiruddin |
| author_facet | Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Tengku Zakaria, Tengku Muhamad Faris Syafiq Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Ahmed, Qamar Uddin Zakaria, Zainul Amiruddin |
| author_sort | Bakhtiar, M. Taher |
| building | IIUM Repository |
| collection | Online Access |
| description | Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of alpha-mangostin, a major xanthone component isolated from the stem bark of G. malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPAR gamma, GLUT4, and leptin expressions. alpha-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation. Cells treated with 50 mu M of alpha-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells. Analyses of 2-deoxy-D-[H-3] glucose uptake activity showed that alpha-mangostin significantly improved the glucose uptake (P < 0.05) with highest activity found at 25 mu M. In addition, alpha-mangostin increased the amount of free fatty acids (FFA) released. The highest glycerol release level was observed at 50 mu M of alpha-mangostin. qRT-PCR analysis showed reduced lipid accumulation via inhibition of PPAR gamma gene expression. Induction of glucose uptake and free fatty acid release by alpha-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. These evidences propose that alpha-mangostin might be possible candidate for the effective management of obesity in future. |
| first_indexed | 2025-11-14T16:02:58Z |
| format | Article |
| id | iium-42492 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T16:02:58Z |
| publishDate | 2015 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-424922021-01-27T02:36:58Z http://irep.iium.edu.my/42492/ Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Tengku Zakaria, Tengku Muhamad Faris Syafiq Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Ahmed, Qamar Uddin Zakaria, Zainul Amiruddin RS Pharmacy and materia medica Obesity has been often associated with the occurrence of cardiovascular diseases, type 2 diabetes, and cancer. The development of obesity is also accompanied by significant differentiation of preadipocytes into adipocytes. In this study, we investigated the activity of alpha-mangostin, a major xanthone component isolated from the stem bark of G. malaccensis, on glucose uptake and adipocyte differentiation of 3T3-L1 cells focusing on PPAR gamma, GLUT4, and leptin expressions. alpha-Mangostin was found to inhibit cytoplasmic lipid accumulation and adipogenic differentiation. Cells treated with 50 mu M of alpha-mangostin reduced intracellular fat accumulation dose-dependently up to 44.4% relative to MDI-treated cells. Analyses of 2-deoxy-D-[H-3] glucose uptake activity showed that alpha-mangostin significantly improved the glucose uptake (P < 0.05) with highest activity found at 25 mu M. In addition, alpha-mangostin increased the amount of free fatty acids (FFA) released. The highest glycerol release level was observed at 50 mu M of alpha-mangostin. qRT-PCR analysis showed reduced lipid accumulation via inhibition of PPAR gamma gene expression. Induction of glucose uptake and free fatty acid release by alpha-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. These evidences propose that alpha-mangostin might be possible candidate for the effective management of obesity in future. Hindawi Publishing Corporation 2015-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42492/1/Alpha_mangostin_ECAM.pdf application/pdf en http://irep.iium.edu.my/42492/4/42492_Alpha-mangostin%20improves%20glucose%20uptake%20and%20inhibits_Scopus.pdf application/pdf en http://irep.iium.edu.my/42492/5/42492_Alpha-mangostin%20improves%20glucose%20uptake%20and%20inhibits_WOS.pdf Bakhtiar, M. Taher and Mohamed Amiroudine, Mohamed Zaffar Ali and Tengku Zakaria, Tengku Muhamad Faris Syafiq and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Kaderi, Mohd Arifin and Ahmed, Qamar Uddin and Zakaria, Zainul Amiruddin (2015) Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions. Evidence-Based Complementary and Alternative Medicine, 15. pp. 1-9. ISSN 1741-427X https://www.hindawi.com/journals/ecam/2015/740238/ 10.1155/2015/740238 |
| spellingShingle | RS Pharmacy and materia medica Bakhtiar, M. Taher Mohamed Amiroudine, Mohamed Zaffar Ali Tengku Zakaria, Tengku Muhamad Faris Syafiq Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Ahmed, Qamar Uddin Zakaria, Zainul Amiruddin Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions |
| title | Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions |
| title_full | Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions |
| title_fullStr | Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions |
| title_full_unstemmed | Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions |
| title_short | Alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells via PPAR gamma, GLUT4, and leptin expressions |
| title_sort | alpha-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3t3-l1 cells via ppar gamma, glut4, and leptin expressions |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/42492/ http://irep.iium.edu.my/42492/ http://irep.iium.edu.my/42492/ http://irep.iium.edu.my/42492/1/Alpha_mangostin_ECAM.pdf http://irep.iium.edu.my/42492/4/42492_Alpha-mangostin%20improves%20glucose%20uptake%20and%20inhibits_Scopus.pdf http://irep.iium.edu.my/42492/5/42492_Alpha-mangostin%20improves%20glucose%20uptake%20and%20inhibits_WOS.pdf |