In vitro wound healing activity of sapium indicum willd leaf extracts
Background: Sapium indicum Willd (Euphorbiaceae) is traditionally used to accelerate wound healing process in Malay community. Objective: To evaluate wound healing potential of aqueous (AESI) and ethanolic extract of S. indicum leaves (EESI) using cell migration model. Methods: AESI and EESI were...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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African Ethnomedicines Network
2015
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/42490/ http://irep.iium.edu.my/42490/1/Huwaida_wound_healing.pdf http://irep.iium.edu.my/42490/4/42490__WOS_and_SCOPUS.pdf |
| _version_ | 1848782281423978496 |
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| author | Abdul Aziz, Huwaida Bakhtiar, M. Taher Darnis, Deny Susanti Zakaria, Zainul Amiruddin |
| author_facet | Abdul Aziz, Huwaida Bakhtiar, M. Taher Darnis, Deny Susanti Zakaria, Zainul Amiruddin |
| author_sort | Abdul Aziz, Huwaida |
| building | IIUM Repository |
| collection | Online Access |
| description | Background: Sapium indicum Willd (Euphorbiaceae) is traditionally used to accelerate wound healing process in Malay community.
Objective: To evaluate wound healing potential of aqueous (AESI) and ethanolic extract of S. indicum leaves (EESI) using cell migration model.
Methods: AESI and EESI were prepared using maceration techniques. The extracts were subjected to phytochemical screening, cytotoxicity
(MTT assay) and scratch assay.
Results: AESI contained saponins and tannins while EESI contained triterpenes, tannins and proteins. Based on MTT assay, AESI and EESI
exhibited IC50 = 16.3 and 70.9 μg/mL, respectively. In the scratch assay, EESI caused significant (P< 0.05) concentration-dependent migration
on 3T3 L1 cells whereas AESI exerted concentration-independent effect.
Conclusion: The leaves of S. indicum exhibited wound healing potential and contained phytochemicals that may contribute to the activity. These
findings would support potential use of S. indicum as wound healing plant. |
| first_indexed | 2025-11-14T16:02:58Z |
| format | Article |
| id | iium-42490 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:02:58Z |
| publishDate | 2015 |
| publisher | African Ethnomedicines Network |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-424902016-05-16T02:30:07Z http://irep.iium.edu.my/42490/ In vitro wound healing activity of sapium indicum willd leaf extracts Abdul Aziz, Huwaida Bakhtiar, M. Taher Darnis, Deny Susanti Zakaria, Zainul Amiruddin RS Pharmacy and materia medica Background: Sapium indicum Willd (Euphorbiaceae) is traditionally used to accelerate wound healing process in Malay community. Objective: To evaluate wound healing potential of aqueous (AESI) and ethanolic extract of S. indicum leaves (EESI) using cell migration model. Methods: AESI and EESI were prepared using maceration techniques. The extracts were subjected to phytochemical screening, cytotoxicity (MTT assay) and scratch assay. Results: AESI contained saponins and tannins while EESI contained triterpenes, tannins and proteins. Based on MTT assay, AESI and EESI exhibited IC50 = 16.3 and 70.9 μg/mL, respectively. In the scratch assay, EESI caused significant (P< 0.05) concentration-dependent migration on 3T3 L1 cells whereas AESI exerted concentration-independent effect. Conclusion: The leaves of S. indicum exhibited wound healing potential and contained phytochemicals that may contribute to the activity. These findings would support potential use of S. indicum as wound healing plant. African Ethnomedicines Network 2015-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42490/1/Huwaida_wound_healing.pdf application/pdf en http://irep.iium.edu.my/42490/4/42490__WOS_and_SCOPUS.pdf Abdul Aziz, Huwaida and Bakhtiar, M. Taher and Darnis, Deny Susanti and Zakaria, Zainul Amiruddin (2015) In vitro wound healing activity of sapium indicum willd leaf extracts. African Journal of Traditional, Complementary and Alternative Medicines, 12 (2). pp. 126-132. ISSN 0189-6016 http://journals.sfu.ca/africanem/index.php/ajtcam/index http://dx.doi.org/10.4314/ajtcam.v12i2.19 |
| spellingShingle | RS Pharmacy and materia medica Abdul Aziz, Huwaida Bakhtiar, M. Taher Darnis, Deny Susanti Zakaria, Zainul Amiruddin In vitro wound healing activity of sapium indicum willd leaf extracts |
| title | In vitro wound healing activity of sapium indicum willd leaf extracts |
| title_full | In vitro wound healing activity of sapium indicum willd leaf extracts |
| title_fullStr | In vitro wound healing activity of sapium indicum willd leaf extracts |
| title_full_unstemmed | In vitro wound healing activity of sapium indicum willd leaf extracts |
| title_short | In vitro wound healing activity of sapium indicum willd leaf extracts |
| title_sort | in vitro wound healing activity of sapium indicum willd leaf extracts |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/42490/ http://irep.iium.edu.my/42490/ http://irep.iium.edu.my/42490/ http://irep.iium.edu.my/42490/1/Huwaida_wound_healing.pdf http://irep.iium.edu.my/42490/4/42490__WOS_and_SCOPUS.pdf |