Effects of process parameters on spray drying of recombinant bromelain

Bromelain is a plant protease with diverse industrial, analytical and pharmacological uses. This research work is aimed at evaluating the effects of some process parameters (inlet temperature, pump and aspirator settings) on spray drying of recombinant bromelain using full factorial design of experi...

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Main Authors: Muntari, Bala, Amid, Azura, Jami, Mohammed Saedi, Mel, Maizirwan, Mohd. Salleh, Hamzah
Format: Proceeding Paper
Language:English
Published: 2013
Subjects:
Online Access:http://irep.iium.edu.my/42415/
http://irep.iium.edu.my/42415/1/ICBIOE_Bala_cover.pdf
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author Muntari, Bala
Amid, Azura
Jami, Mohammed Saedi
Mel, Maizirwan
Mohd. Salleh, Hamzah
author_facet Muntari, Bala
Amid, Azura
Jami, Mohammed Saedi
Mel, Maizirwan
Mohd. Salleh, Hamzah
author_sort Muntari, Bala
building IIUM Repository
collection Online Access
description Bromelain is a plant protease with diverse industrial, analytical and pharmacological uses. This research work is aimed at evaluating the effects of some process parameters (inlet temperature, pump and aspirator settings) on spray drying of recombinant bromelain using full factorial design of experiments. The experiments were conducted in Buchi B-290 mini spray dryer using maltodextrin as carrier agent. The results obtained revealed that the interaction and linear coefficients wer e statistically significant coupled with existence of good conformity between the experimental and the theoretical values predicted by the model. The precise optimum conditions obtained (aspirator setting, 90 (%); inlet temperature, 110 ºC; and pump setting, 12.5 %) generated the maximum activity of 78.08±0.07 U/ml (equivalent to residual activity of about 92.64±0.09) for the spray dried recombinant bromelain. This implies that the study has effectively produced viable and well formulated spray dried recombinant bromelain under optimized conditions.
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format Proceeding Paper
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institution International Islamic University Malaysia
institution_category Local University
language English
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publishDate 2013
recordtype eprints
repository_type Digital Repository
spelling iium-424152021-06-29T03:32:18Z http://irep.iium.edu.my/42415/ Effects of process parameters on spray drying of recombinant bromelain Muntari, Bala Amid, Azura Jami, Mohammed Saedi Mel, Maizirwan Mohd. Salleh, Hamzah TP248.13 Biotechnology Bromelain is a plant protease with diverse industrial, analytical and pharmacological uses. This research work is aimed at evaluating the effects of some process parameters (inlet temperature, pump and aspirator settings) on spray drying of recombinant bromelain using full factorial design of experiments. The experiments were conducted in Buchi B-290 mini spray dryer using maltodextrin as carrier agent. The results obtained revealed that the interaction and linear coefficients wer e statistically significant coupled with existence of good conformity between the experimental and the theoretical values predicted by the model. The precise optimum conditions obtained (aspirator setting, 90 (%); inlet temperature, 110 ºC; and pump setting, 12.5 %) generated the maximum activity of 78.08±0.07 U/ml (equivalent to residual activity of about 92.64±0.09) for the spray dried recombinant bromelain. This implies that the study has effectively produced viable and well formulated spray dried recombinant bromelain under optimized conditions. 2013 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/42415/1/ICBIOE_Bala_cover.pdf Muntari, Bala and Amid, Azura and Jami, Mohammed Saedi and Mel, Maizirwan and Mohd. Salleh, Hamzah (2013) Effects of process parameters on spray drying of recombinant bromelain. In: 3rd International Conference on Biotechnology Engineering (ICBIoE 2013), 2-4 July 2013, Kuala Lumpur, Malaysia. http://www.iium.edu.my/icbioe/2013/
spellingShingle TP248.13 Biotechnology
Muntari, Bala
Amid, Azura
Jami, Mohammed Saedi
Mel, Maizirwan
Mohd. Salleh, Hamzah
Effects of process parameters on spray drying of recombinant bromelain
title Effects of process parameters on spray drying of recombinant bromelain
title_full Effects of process parameters on spray drying of recombinant bromelain
title_fullStr Effects of process parameters on spray drying of recombinant bromelain
title_full_unstemmed Effects of process parameters on spray drying of recombinant bromelain
title_short Effects of process parameters on spray drying of recombinant bromelain
title_sort effects of process parameters on spray drying of recombinant bromelain
topic TP248.13 Biotechnology
url http://irep.iium.edu.my/42415/
http://irep.iium.edu.my/42415/
http://irep.iium.edu.my/42415/1/ICBIOE_Bala_cover.pdf