Optimisation of hydrolysis conditions for ethanol production from sorghum starch
The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of s...
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| Format: | Article |
| Language: | English |
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The Institution of Engineers
2010
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| Online Access: | http://irep.iium.edu.my/4240/ http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf |
| _version_ | 1848776296783413248 |
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| author | Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli |
| author_facet | Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli |
| author_sort | Nadir, Najiah |
| building | IIUM Repository |
| collection | Online Access |
| description | The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of substrate, liquefaction and saccharification temperature, liquefaction and saccharification time, and amount of α-amylase and glucoamylase enzymes. The screening of significant hydrolysis factors were done by using the two-level factorial design (TLFD) under the factorial design (FD). The results indicated that the liquefaction and saccharification temperature, and amount of glucoamylase enzyme were found to be the major factors for further optimisation. The major factors for hydrolysis were optimised by the central composite design (CCD) under the response surface method (RSM). The analysis of variance(ANOVA) result showed that glucoamylase enzyme (p < 0.0021) and saccharification temperature (p < 0.0181) were significant factors for hydrolysis of sorghum starch. Also, the statistical analysis showed that the optimum dextrose equivalent (69.07% (g/g)) were obtained at 90°C of liquefaction temperature, 47°C of saccharification temperature, and 0.24% (v/w) of glucoamylase enzyme. |
| first_indexed | 2025-11-14T14:27:50Z |
| format | Article |
| id | iium-4240 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:27:50Z |
| publishDate | 2010 |
| publisher | The Institution of Engineers |
| recordtype | eprints |
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| spelling | iium-42402011-09-22T07:32:16Z http://irep.iium.edu.my/4240/ Optimisation of hydrolysis conditions for ethanol production from sorghum starch Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli TP155 Chemical engineering TP248.13 Biotechnology The conversion of starch to sugar can be achieved by hydrolysis process. The two-step enzymatic hydrolysis of sweet sorghum was performed by commercially available α-amylase and glucoamylase. An optimisation study was carried out to optimise the factors of the hydrolysis process, namely, amount of substrate, liquefaction and saccharification temperature, liquefaction and saccharification time, and amount of α-amylase and glucoamylase enzymes. The screening of significant hydrolysis factors were done by using the two-level factorial design (TLFD) under the factorial design (FD). The results indicated that the liquefaction and saccharification temperature, and amount of glucoamylase enzyme were found to be the major factors for further optimisation. The major factors for hydrolysis were optimised by the central composite design (CCD) under the response surface method (RSM). The analysis of variance(ANOVA) result showed that glucoamylase enzyme (p < 0.0021) and saccharification temperature (p < 0.0181) were significant factors for hydrolysis of sorghum starch. Also, the statistical analysis showed that the optimum dextrose equivalent (69.07% (g/g)) were obtained at 90°C of liquefaction temperature, 47°C of saccharification temperature, and 0.24% (v/w) of glucoamylase enzyme. The Institution of Engineers 2010-09 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf Nadir, Najiah and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Mohd Yunus, Rosli (2010) Optimisation of hydrolysis conditions for ethanol production from sorghum starch. Journal - The Institution of Engineers, Malaysia, 71 (3). pp. 26-34. ISSN 0126-513X http://www.myiem.org.my/content/iem_journal_2010-181.aspx |
| spellingShingle | TP155 Chemical engineering TP248.13 Biotechnology Nadir, Najiah Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yunus, Rosli Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title | Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_full | Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_fullStr | Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_full_unstemmed | Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_short | Optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| title_sort | optimisation of hydrolysis conditions for ethanol production from sorghum starch |
| topic | TP155 Chemical engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/4240/ http://irep.iium.edu.my/4240/ http://irep.iium.edu.my/4240/1/5._Nadir_et_al_-_Journal_IEM_Malaysia.pdf |