Optimization of pre-transplantation conditions to enhance the efficacy of mesenchymal stem cells
Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major li...
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| Format: | Article |
| Language: | English English |
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Ivyspring International Publisher.
2015
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| Online Access: | http://irep.iium.edu.my/42326/ http://irep.iium.edu.my/42326/1/Int_J_Biol_Sci_2015_11_324-334_DoI_10_7150_ijbs_10567.pdf http://irep.iium.edu.my/42326/4/42326_Optimization%20of%20pre-transplantation%20conditions_SCOPUS.pdf |
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| author | Haque, Nazmul Abu Kasim, Noor Hayaty Rahman, Mohammad Tariqur |
| author_facet | Haque, Nazmul Abu Kasim, Noor Hayaty Rahman, Mohammad Tariqur |
| author_sort | Haque, Nazmul |
| building | IIUM Repository |
| collection | Online Access |
| description | Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major limitations which were linked to early cellular ageing, loss of chemokine markers during ex
vivo expansion, and hyper-immunogenicity to xeno-contaminated MSCs. These problems can be minimized by ex vivo expansion of MSCs in hypoxic culture condition using well defined or xeno-free media i.e., media supplemented with growth factors, human serum or platelet lysate. In
addition to ex vivo expansion in hypoxic culture condition using well defined media, this review article describes the potentials of transient adaptation of expanded MSCs in autologous serum supplemented medium prior to transplantation for long term regenerative benefits. Such transient adaptation in autologous serum supplemented medium may help to increase chemokine receptor expression and tissue specific differentiation of ex vivo expanded MSCs, thus would provide long term regenerative benefits. |
| first_indexed | 2025-11-14T16:02:31Z |
| format | Article |
| id | iium-42326 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:02:31Z |
| publishDate | 2015 |
| publisher | Ivyspring International Publisher. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-423262017-11-08T04:18:08Z http://irep.iium.edu.my/42326/ Optimization of pre-transplantation conditions to enhance the efficacy of mesenchymal stem cells Haque, Nazmul Abu Kasim, Noor Hayaty Rahman, Mohammad Tariqur RM Therapeutics. Pharmacology Mesenchymal stem cells (MSCs) are considered a potential tool for cell based regenerative therapy due to their immunomodulatory property, differentiation potentials, trophic activity as well as large donor pool. Poor engraftment and short term survival of transplanted MSCs are recognized as major limitations which were linked to early cellular ageing, loss of chemokine markers during ex vivo expansion, and hyper-immunogenicity to xeno-contaminated MSCs. These problems can be minimized by ex vivo expansion of MSCs in hypoxic culture condition using well defined or xeno-free media i.e., media supplemented with growth factors, human serum or platelet lysate. In addition to ex vivo expansion in hypoxic culture condition using well defined media, this review article describes the potentials of transient adaptation of expanded MSCs in autologous serum supplemented medium prior to transplantation for long term regenerative benefits. Such transient adaptation in autologous serum supplemented medium may help to increase chemokine receptor expression and tissue specific differentiation of ex vivo expanded MSCs, thus would provide long term regenerative benefits. Ivyspring International Publisher. 2015 Article PeerReviewed application/pdf en http://irep.iium.edu.my/42326/1/Int_J_Biol_Sci_2015_11_324-334_DoI_10_7150_ijbs_10567.pdf application/pdf en http://irep.iium.edu.my/42326/4/42326_Optimization%20of%20pre-transplantation%20conditions_SCOPUS.pdf Haque, Nazmul and Abu Kasim, Noor Hayaty and Rahman, Mohammad Tariqur (2015) Optimization of pre-transplantation conditions to enhance the efficacy of mesenchymal stem cells. International Journal of Biological Sciences, 11 (3). pp. 324-334. ISSN 1449-2288 http://www.ijbs.com/ 10.7150/ijbs.10567 |
| spellingShingle | RM Therapeutics. Pharmacology Haque, Nazmul Abu Kasim, Noor Hayaty Rahman, Mohammad Tariqur Optimization of pre-transplantation conditions to enhance the efficacy of mesenchymal stem cells |
| title | Optimization of pre-transplantation conditions to
enhance the efficacy of mesenchymal stem cells |
| title_full | Optimization of pre-transplantation conditions to
enhance the efficacy of mesenchymal stem cells |
| title_fullStr | Optimization of pre-transplantation conditions to
enhance the efficacy of mesenchymal stem cells |
| title_full_unstemmed | Optimization of pre-transplantation conditions to
enhance the efficacy of mesenchymal stem cells |
| title_short | Optimization of pre-transplantation conditions to
enhance the efficacy of mesenchymal stem cells |
| title_sort | optimization of pre-transplantation conditions to
enhance the efficacy of mesenchymal stem cells |
| topic | RM Therapeutics. Pharmacology |
| url | http://irep.iium.edu.my/42326/ http://irep.iium.edu.my/42326/ http://irep.iium.edu.my/42326/ http://irep.iium.edu.my/42326/1/Int_J_Biol_Sci_2015_11_324-334_DoI_10_7150_ijbs_10567.pdf http://irep.iium.edu.my/42326/4/42326_Optimization%20of%20pre-transplantation%20conditions_SCOPUS.pdf |