Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor
The production of monoclonal antibody (IgM) for anti-cancer by fermentation of hybridoma cell in benchtop bioreactor has been investigated. The main target of this study was to increase the percentage of cell viability and to achieve high yield of IgM. There are many ways that can be applied to impr...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/4232/ http://irep.iium.edu.my/4232/1/Paper_SCP.pdf |
| _version_ | 1848776295692894208 |
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| author | Mel, Maizirwan Arifin, Mohd Azmir Abdul Karim, Mohamed Ismail Yusof, Faridah |
| author_facet | Mel, Maizirwan Arifin, Mohd Azmir Abdul Karim, Mohamed Ismail Yusof, Faridah |
| author_sort | Mel, Maizirwan |
| building | IIUM Repository |
| collection | Online Access |
| description | The production of monoclonal antibody (IgM) for anti-cancer by fermentation of hybridoma cell in benchtop bioreactor has been investigated. The main target of this study was to increase the percentage of cell viability and to achieve high yield of IgM. There are many ways that can be applied to improve the process and one of them is by choosing the correct Six Sigma’s Design of Experiment (DOE). In this study, Taguchi’s Experimental Design has been applied to achieve high percentage of cell viability in the fermentation experiment. The process capability of this study was later analyzed by using the MiniTab Software (Ver. 14). Analysis has shown that this study has fulfilled a minimum target of process improvement as indicated by the Cp and Cpk value of 0.90 and 0.86. This proved that the fermentation experiment has achieved cell viability of more than 80% throughout the study. |
| first_indexed | 2025-11-14T14:27:49Z |
| format | Proceeding Paper |
| id | iium-4232 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:27:49Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-42322011-10-05T06:42:11Z http://irep.iium.edu.my/4232/ Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor Mel, Maizirwan Arifin, Mohd Azmir Abdul Karim, Mohamed Ismail Yusof, Faridah TP155 Chemical engineering TP248.13 Biotechnology The production of monoclonal antibody (IgM) for anti-cancer by fermentation of hybridoma cell in benchtop bioreactor has been investigated. The main target of this study was to increase the percentage of cell viability and to achieve high yield of IgM. There are many ways that can be applied to improve the process and one of them is by choosing the correct Six Sigma’s Design of Experiment (DOE). In this study, Taguchi’s Experimental Design has been applied to achieve high percentage of cell viability in the fermentation experiment. The process capability of this study was later analyzed by using the MiniTab Software (Ver. 14). Analysis has shown that this study has fulfilled a minimum target of process improvement as indicated by the Cp and Cpk value of 0.90 and 0.86. This proved that the fermentation experiment has achieved cell viability of more than 80% throughout the study. 2011 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/4232/1/Paper_SCP.pdf Mel, Maizirwan and Arifin, Mohd Azmir and Abdul Karim, Mohamed Ismail and Yusof, Faridah (2011) Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. |
| spellingShingle | TP155 Chemical engineering TP248.13 Biotechnology Mel, Maizirwan Arifin, Mohd Azmir Abdul Karim, Mohamed Ismail Yusof, Faridah Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor |
| title | Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor |
| title_full | Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor |
| title_fullStr | Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor |
| title_full_unstemmed | Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor |
| title_short | Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor |
| title_sort | simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor |
| topic | TP155 Chemical engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/4232/ http://irep.iium.edu.my/4232/1/Paper_SCP.pdf |