Preliminary study on biohydrogen production by bacteria from sago waste
Sago waste which has not been fully utilized is a potential substrate for biotechnology production. In lieu of that, a study focused on utilizing the waste to produce biohydrogen as the green- technology product was conducted. This study is concerned with the hydrogen gas production by Escherichia c...
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2014
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| Online Access: | http://irep.iium.edu.my/42264/ http://irep.iium.edu.my/42264/2/Pse170_vetted_formatted_final.pdf http://irep.iium.edu.my/42264/5/programme_booklet.pdf |
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| author | Mohamad Puad, Noor Illi Sulaiman, Sarina Azmi, Azlin Suhaida Shamsudin, Yusra Mel, Maizirwan |
| author_facet | Mohamad Puad, Noor Illi Sulaiman, Sarina Azmi, Azlin Suhaida Shamsudin, Yusra Mel, Maizirwan |
| author_sort | Mohamad Puad, Noor Illi |
| building | IIUM Repository |
| collection | Online Access |
| description | Sago waste which has not been fully utilized is a potential substrate for biotechnology production. In lieu of that, a study focused on utilizing the waste to produce biohydrogen as the green- technology product was conducted. This study is concerned with the hydrogen gas production by Escherichia coli (E. coli) using dark fermentation by utilizing sago waste as the substrate. Acid pre-treatment process for sago waste was executed and the content of reducing sugar was analysed using Dinitrosalicylic acid (DNS) method. The glucose content after pre-treatment process was 2.150 g/L when treated at the temperature of 90C for 40 minutes. Dark fermentation process was performed for 14 hours and three factors which were inoculum size (10-30%), pH (6-8) and temperature (28-40C) were investigated. The design of experiment was done using central composite design (Design Expert v6.0.8). Maximum yield of 2.22 mol H2/ mol glucose was obtained from Run 8 at processing parameters of pH 6, temperature of 40C and 30% inoculum size. The findings of this study can be applied in production of biohydrogen at a wider range of parameters (pH, temperature, inoculum size) for further studies. |
| first_indexed | 2025-11-14T16:02:21Z |
| format | Proceeding Paper |
| id | iium-42264 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:02:21Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-422642018-06-18T13:39:05Z http://irep.iium.edu.my/42264/ Preliminary study on biohydrogen production by bacteria from sago waste Mohamad Puad, Noor Illi Sulaiman, Sarina Azmi, Azlin Suhaida Shamsudin, Yusra Mel, Maizirwan T Technology (General) Sago waste which has not been fully utilized is a potential substrate for biotechnology production. In lieu of that, a study focused on utilizing the waste to produce biohydrogen as the green- technology product was conducted. This study is concerned with the hydrogen gas production by Escherichia coli (E. coli) using dark fermentation by utilizing sago waste as the substrate. Acid pre-treatment process for sago waste was executed and the content of reducing sugar was analysed using Dinitrosalicylic acid (DNS) method. The glucose content after pre-treatment process was 2.150 g/L when treated at the temperature of 90C for 40 minutes. Dark fermentation process was performed for 14 hours and three factors which were inoculum size (10-30%), pH (6-8) and temperature (28-40C) were investigated. The design of experiment was done using central composite design (Design Expert v6.0.8). Maximum yield of 2.22 mol H2/ mol glucose was obtained from Run 8 at processing parameters of pH 6, temperature of 40C and 30% inoculum size. The findings of this study can be applied in production of biohydrogen at a wider range of parameters (pH, temperature, inoculum size) for further studies. 2014-10 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/42264/2/Pse170_vetted_formatted_final.pdf application/pdf en http://irep.iium.edu.my/42264/5/programme_booklet.pdf Mohamad Puad, Noor Illi and Sulaiman, Sarina and Azmi, Azlin Suhaida and Shamsudin, Yusra and Mel, Maizirwan (2014) Preliminary study on biohydrogen production by bacteria from sago waste. In: 27th Symposium of Malaysian Chemical Engineers (SOMChE 2014) in conjunction with 21st Regional Symposium on Chemical Engineering (RSCE 2014), 28th-30th Oct. 2014, Taylor's University Lakeside Campus, Subang Jaya, Selangor. (Unpublished) |
| spellingShingle | T Technology (General) Mohamad Puad, Noor Illi Sulaiman, Sarina Azmi, Azlin Suhaida Shamsudin, Yusra Mel, Maizirwan Preliminary study on biohydrogen production by bacteria from sago waste |
| title | Preliminary study on biohydrogen production by bacteria from sago waste |
| title_full | Preliminary study on biohydrogen production by bacteria from sago waste |
| title_fullStr | Preliminary study on biohydrogen production by bacteria from sago waste |
| title_full_unstemmed | Preliminary study on biohydrogen production by bacteria from sago waste |
| title_short | Preliminary study on biohydrogen production by bacteria from sago waste |
| title_sort | preliminary study on biohydrogen production by bacteria from sago waste |
| topic | T Technology (General) |
| url | http://irep.iium.edu.my/42264/ http://irep.iium.edu.my/42264/2/Pse170_vetted_formatted_final.pdf http://irep.iium.edu.my/42264/5/programme_booklet.pdf |