Study of cells attachment using impedance spectroscopy technique
In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwel...
| Main Authors: | , , , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/4226/ http://irep.iium.edu.my/4226/1/ASME2011.pdf |
| _version_ | 1848776294483886080 |
|---|---|
| author | Arifuzzaman, S. M. Nordin, Anis Nurashikin Mel, Maizirwan Spray, David Voiculescu, Ioana |
| author_facet | Arifuzzaman, S. M. Nordin, Anis Nurashikin Mel, Maizirwan Spray, David Voiculescu, Ioana |
| author_sort | Arifuzzaman, S. M. |
| building | IIUM Repository |
| collection | Online Access |
| description | In this paper we report on the electrical impedance
spectroscopy characterization of the attachment of two
mammalian cell lines: endothelial cells (RFPEC) and fibroblast
cells (V79). For electrical cell–substrate impedance sensing
(ECIS) of the endothelial cells a commercially available eightwell
cell culture impedance array (ECIS-8W1E) was used. The
impedance measurements were recorded with cell culture
medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL MultiphysicsTM, a commercially available modeling package. |
| first_indexed | 2025-11-14T14:27:48Z |
| format | Proceeding Paper |
| id | iium-4226 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:27:48Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-42262011-09-12T09:10:55Z http://irep.iium.edu.my/4226/ Study of cells attachment using impedance spectroscopy technique Arifuzzaman, S. M. Nordin, Anis Nurashikin Mel, Maizirwan Spray, David Voiculescu, Ioana T175 Industrial research. Research and development TP248.13 Biotechnology In this paper we report on the electrical impedance spectroscopy characterization of the attachment of two mammalian cell lines: endothelial cells (RFPEC) and fibroblast cells (V79). For electrical cell–substrate impedance sensing (ECIS) of the endothelial cells a commercially available eightwell cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cells or fibroblast cells in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements were also simulated using COMSOL MultiphysicsTM, a commercially available modeling package. 2011 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/4226/1/ASME2011.pdf Arifuzzaman, S. M. and Nordin, Anis Nurashikin and Mel, Maizirwan and Spray, David and Voiculescu, Ioana (2011) Study of cells attachment using impedance spectroscopy technique. In: ASME International Mechanical Engineering Congress and Exposition (ASME 2011), 11-17 November 2011, Denver Colorado. (In Press) http://www.asmeconferences.org/congress2011/ |
| spellingShingle | T175 Industrial research. Research and development TP248.13 Biotechnology Arifuzzaman, S. M. Nordin, Anis Nurashikin Mel, Maizirwan Spray, David Voiculescu, Ioana Study of cells attachment using impedance spectroscopy technique |
| title | Study of cells attachment using impedance spectroscopy technique |
| title_full | Study of cells attachment using impedance spectroscopy technique |
| title_fullStr | Study of cells attachment using impedance spectroscopy technique |
| title_full_unstemmed | Study of cells attachment using impedance spectroscopy technique |
| title_short | Study of cells attachment using impedance spectroscopy technique |
| title_sort | study of cells attachment using impedance spectroscopy technique |
| topic | T175 Industrial research. Research and development TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/4226/ http://irep.iium.edu.my/4226/ http://irep.iium.edu.my/4226/1/ASME2011.pdf |