Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors

The present work was aimed towards the evaluation of effects of the physical and chemical factors on laccase production by white rot fungus, Resinicium bicolor ATCC 64897 under submerged condition in shake flask using PlackettBurman design. R.bicolor potentially detoxifies ground tire rubber (G...

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Main Authors: Shah , Harmen, Yusof, Faridah, Alam, Md. Zahangir
Format: Proceeding Paper
Language:English
Published: 2014
Subjects:
Online Access:http://irep.iium.edu.my/41885/
http://irep.iium.edu.my/41885/1/41885.pdf
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author Shah , Harmen
Yusof, Faridah
Alam, Md. Zahangir
author_facet Shah , Harmen
Yusof, Faridah
Alam, Md. Zahangir
author_sort Shah , Harmen
building IIUM Repository
collection Online Access
description The present work was aimed towards the evaluation of effects of the physical and chemical factors on laccase production by white rot fungus, Resinicium bicolor ATCC 64897 under submerged condition in shake flask using PlackettBurman design. R.bicolor potentially detoxifies ground tire rubber (GTR) and facilitates growth of Thiobacillus ferrooxidans, a bacterium that performs devulcanization of GTR but is sensitive towards rubber additives present in GTR. In this experiment out of 11 factors screened, copper sulphate and yellow flame bark powder were found to be the most significant factors affecting the laccase production. The study indicated that the screened factors can be optimized for enhanced laccase production and can potentially be utilized for attaining maximum detoxification of GTR and hence further devulcanization.
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format Proceeding Paper
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institution International Islamic University Malaysia
institution_category Local University
language English
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publishDate 2014
recordtype eprints
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spelling iium-418852020-10-30T01:48:55Z http://irep.iium.edu.my/41885/ Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors Shah , Harmen Yusof, Faridah Alam, Md. Zahangir TP248.13 Biotechnology The present work was aimed towards the evaluation of effects of the physical and chemical factors on laccase production by white rot fungus, Resinicium bicolor ATCC 64897 under submerged condition in shake flask using PlackettBurman design. R.bicolor potentially detoxifies ground tire rubber (GTR) and facilitates growth of Thiobacillus ferrooxidans, a bacterium that performs devulcanization of GTR but is sensitive towards rubber additives present in GTR. In this experiment out of 11 factors screened, copper sulphate and yellow flame bark powder were found to be the most significant factors affecting the laccase production. The study indicated that the screened factors can be optimized for enhanced laccase production and can potentially be utilized for attaining maximum detoxification of GTR and hence further devulcanization. 2014-03 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/41885/1/41885.pdf Shah , Harmen and Yusof, Faridah and Alam, Md. Zahangir (2014) Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors. In: International Conference on Advances in Civil, Structural, Environmental and Bio-Technology (CSEB 2014), 8-9 March 2014, Kuala Lumpur. https://www.seekdl.org/conferences/paper/details/2787.html
spellingShingle TP248.13 Biotechnology
Shah , Harmen
Yusof, Faridah
Alam, Md. Zahangir
Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors
title Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors
title_full Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors
title_fullStr Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors
title_full_unstemmed Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors
title_short Production of laccase by Resinicium bicolor in submerged cultures: application of the Plackett-Burman experimental design to screen major factors
title_sort production of laccase by resinicium bicolor in submerged cultures: application of the plackett-burman experimental design to screen major factors
topic TP248.13 Biotechnology
url http://irep.iium.edu.my/41885/
http://irep.iium.edu.my/41885/
http://irep.iium.edu.my/41885/1/41885.pdf