High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector
Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). It is a class of drug that provides analgesic and antipyretic (fever reducing effect) and in higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic ac...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2014
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| Online Access: | http://irep.iium.edu.my/41453/ http://irep.iium.edu.my/41453/1/IPharME-2014.pdf |
| _version_ | 1848782126422425600 |
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| author | Helal Uddin, A.B.M. Mohamad, Huda Jamilah Alaama, Mohammed Amiruddin, Noor Syafawati |
| author_facet | Helal Uddin, A.B.M. Mohamad, Huda Jamilah Alaama, Mohammed Amiruddin, Noor Syafawati |
| author_sort | Helal Uddin, A.B.M. |
| building | IIUM Repository |
| collection | Online Access |
| description | Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). It is a class of drug that provides analgesic and antipyretic (fever reducing effect) and in higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. A simple and sensitive high-performance liquid chromatography-UV detection method was developed for the detection of mefenamic acid. Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 (150 mm x 4.6 mm, i.d 3.5µm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored under 280 nm using variable-wavelength detector. The recovery was found to be 83% for MA. The method was validated for following the CDER (Centre for Drug Evaluation and Research) guideline. Calibration plot were linear within the range from 250 to 5000ng ml-1 with the correlation coefficient (r2) of ≥ 0.99. The result from analysis of quality control of mefenamic acid which were termed as low, medium and high were analysed to get the precision and accuracy. The accuracy for intra-day for low, medium and high were 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient of variance (CV) for intra-day precision for low, medium and high were found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study.
Keyword
Mefenamic acid, HPLC, plasma, protein precipitation technique.
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| first_indexed | 2025-11-14T16:00:30Z |
| format | Proceeding Paper |
| id | iium-41453 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:00:30Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-414532022-11-07T03:59:08Z http://irep.iium.edu.my/41453/ High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector Helal Uddin, A.B.M. Mohamad, Huda Jamilah Alaama, Mohammed Amiruddin, Noor Syafawati QD Chemistry RS Pharmacy and materia medica Mefenamic Acid (MA) is a non-steroidal anti-inflammatory drug (NSAIDs). It is a class of drug that provides analgesic and antipyretic (fever reducing effect) and in higher doses, anti-inflammatory effect. This study is focused to develop a rapid and sensitive method for the detection of mefenamic acid in human plasma. A simple and sensitive high-performance liquid chromatography-UV detection method was developed for the detection of mefenamic acid. Protein precipitation technique using acetonitrile was used. Chromatographic separation was achieved on Agilent Zorbax Eclipse XDB-C18 (150 mm x 4.6 mm, i.d 3.5µm) with a mobile phase consisting of acetonitrile and 2% triethylamine (pH was adjusted to 4.2 with phosphoric acid) in a ratio of 60:40. The retention time for mefenamic acid and diclofenac was 5.4 and 3.9 minutes respectively. The mefenamic acid was monitored under 280 nm using variable-wavelength detector. The recovery was found to be 83% for MA. The method was validated for following the CDER (Centre for Drug Evaluation and Research) guideline. Calibration plot were linear within the range from 250 to 5000ng ml-1 with the correlation coefficient (r2) of ≥ 0.99. The result from analysis of quality control of mefenamic acid which were termed as low, medium and high were analysed to get the precision and accuracy. The accuracy for intra-day for low, medium and high were 99.71%, 93.8% and 89.52% while for inter day were 97.67%, 93.46% and 91.67% respectively. On the other hand, coefficient of variance (CV) for intra-day precision for low, medium and high were found 2.57%, 2.45% and 1.45% and for inter day CV were 3.11%, 5.5% and 4.37% respectively. Diclofenac sodium was used as internal standard for this study. Keyword Mefenamic acid, HPLC, plasma, protein precipitation technique. 2014 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/41453/1/IPharME-2014.pdf Helal Uddin, A.B.M. and Mohamad, Huda Jamilah and Alaama, Mohammed and Amiruddin, Noor Syafawati (2014) High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector. In: International Conference on Pharmaceutical, Medical & Environmental Health Sciences (IPharME, 2014), 17-18 October, 2014, Park Royal, Penang. |
| spellingShingle | QD Chemistry RS Pharmacy and materia medica Helal Uddin, A.B.M. Mohamad, Huda Jamilah Alaama, Mohammed Amiruddin, Noor Syafawati High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector |
| title | High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector |
| title_full | High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector |
| title_fullStr | High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector |
| title_full_unstemmed | High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector |
| title_short | High performance liquid chromatographic determination of Mefenamic acid in human plasma using UV vis detector |
| title_sort | high performance liquid chromatographic determination of mefenamic acid in human plasma using uv vis detector |
| topic | QD Chemistry RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/41453/ http://irep.iium.edu.my/41453/1/IPharME-2014.pdf |