The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis
Our preliminary results indicated that fibrin and poly(lactic co-glycolic acid) (PLGA) hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaff...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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AO Research Institute Davos
2008
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/41404/ http://irep.iium.edu.my/41404/1/v015a04.pdf |
| _version_ | 1848782115983851520 |
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| author | Sha'ban, Munirah Kim, Soon Hee Idrus, Ruszymah Khang, Gilson |
| author_facet | Sha'ban, Munirah Kim, Soon Hee Idrus, Ruszymah Khang, Gilson |
| author_sort | Sha'ban, Munirah |
| building | IIUM Repository |
| collection | Online Access |
| description | Our preliminary results indicated that fibrin and poly(lactic co-glycolic acid) (PLGA) hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaffolds. PLGA scaffolds were soaked carefully, in chondrocyte-fibrin suspension, and polymerized by dropping thrombin-calcium chloride (CaCl2) solution.PLGA-seeded chondrocytes were used as a control.
Resulting constructs were implanted subcutaneously, at the
dorsum of nude mice, for 4 weeks. Macroscopic observation, histological evaluation, gene expression and
sulphated-glycosaminoglycan (sGAG) analyses were
performed at each time point of 1, 2 and 4 weeks postimplantation.Cartilaginous tissue formation in fibrin/PLGA hybrid construct was confirmed by the presence of lacunae and cartilage-isolated cells embedded within basophilic ground substance. Presence of proteoglycan and
glycosaminoglycan (GAG) in fibrin/PLGA hybrid
constructs was confirmed by positive Safranin O and Alcian
Blue staining. Collagen type II exhibited intense
immunopositivity at the pericellular matrices.
Chondrogenic properties were further demonstrated by the
expression of gene encoded cartilage-specific markers,
collagen type II and aggrecan core protein. The sGAG
production in fibrin/PLGA hybrid constructs was higher
than in the PLGA group. In conclusion, fibrin/PLGA hybrid
scaffold promotes cartilaginous tissue formation in vivo and
may serve as a potential cell delivery vehicle and a structural basis for articular cartilage tissue-engineering. |
| first_indexed | 2025-11-14T16:00:20Z |
| format | Article |
| id | iium-41404 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:00:20Z |
| publishDate | 2008 |
| publisher | AO Research Institute Davos |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-414042015-07-01T00:49:37Z http://irep.iium.edu.my/41404/ The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis Sha'ban, Munirah Kim, Soon Hee Idrus, Ruszymah Khang, Gilson RD701 Orthopedics Our preliminary results indicated that fibrin and poly(lactic co-glycolic acid) (PLGA) hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaffolds. PLGA scaffolds were soaked carefully, in chondrocyte-fibrin suspension, and polymerized by dropping thrombin-calcium chloride (CaCl2) solution.PLGA-seeded chondrocytes were used as a control. Resulting constructs were implanted subcutaneously, at the dorsum of nude mice, for 4 weeks. Macroscopic observation, histological evaluation, gene expression and sulphated-glycosaminoglycan (sGAG) analyses were performed at each time point of 1, 2 and 4 weeks postimplantation.Cartilaginous tissue formation in fibrin/PLGA hybrid construct was confirmed by the presence of lacunae and cartilage-isolated cells embedded within basophilic ground substance. Presence of proteoglycan and glycosaminoglycan (GAG) in fibrin/PLGA hybrid constructs was confirmed by positive Safranin O and Alcian Blue staining. Collagen type II exhibited intense immunopositivity at the pericellular matrices. Chondrogenic properties were further demonstrated by the expression of gene encoded cartilage-specific markers, collagen type II and aggrecan core protein. The sGAG production in fibrin/PLGA hybrid constructs was higher than in the PLGA group. In conclusion, fibrin/PLGA hybrid scaffold promotes cartilaginous tissue formation in vivo and may serve as a potential cell delivery vehicle and a structural basis for articular cartilage tissue-engineering. AO Research Institute Davos 2008 Article PeerReviewed application/pdf en http://irep.iium.edu.my/41404/1/v015a04.pdf Sha'ban, Munirah and Kim, Soon Hee and Idrus, Ruszymah and Khang, Gilson (2008) The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis. European Cells and Materials, 15. pp. 41-52. ISSN 1473-2262 http://www.ecmjournal.org/journal/papers/vol015/vol015a04.php |
| spellingShingle | RD701 Orthopedics Sha'ban, Munirah Kim, Soon Hee Idrus, Ruszymah Khang, Gilson The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis |
| title | The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis |
| title_full | The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis |
| title_fullStr | The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis |
| title_full_unstemmed | The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis |
| title_short | The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis |
| title_sort | use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis |
| topic | RD701 Orthopedics |
| url | http://irep.iium.edu.my/41404/ http://irep.iium.edu.my/41404/ http://irep.iium.edu.my/41404/1/v015a04.pdf |