Immobilization of lipase enzyme by low cost material: a statistical approach
Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (AC) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
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World Academy of Science, Engineering and Technology
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/40458/ http://irep.iium.edu.my/40458/1/Paper-Canada.pdf |
| _version_ | 1848781959513243648 |
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| author | Alam, Md. Zahangir Asih, Devi Ratna Salleh, Md. Noor |
| author_facet | Alam, Md. Zahangir Asih, Devi Ratna Salleh, Md. Noor |
| author_sort | Alam, Md. Zahangir |
| building | IIUM Repository |
| collection | Online Access |
| description | Immobilization of lipase enzyme produced from palm
oil mill effluent (POME) by the activated carbon (AC) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy based on a statistical experimental design, including one-factor-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by the
response surface methodology (RSM) based on the face-centered central composite design (FCCCD). On the statistical analysis of the results, the optimum enzyme concentration loading, agitation rate and carbon active dosage were found to be 30 U/ml, 300 rpm and 8 g/L
respectively, with a maximum immobilization activity of 3732.9 U/gAC after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high regression coefficient (R
2) of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05.
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| first_indexed | 2025-11-14T15:57:51Z |
| format | Proceeding Paper |
| id | iium-40458 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:57:51Z |
| publishDate | 2014 |
| publisher | World Academy of Science, Engineering and Technology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-404582018-06-12T07:45:09Z http://irep.iium.edu.my/40458/ Immobilization of lipase enzyme by low cost material: a statistical approach Alam, Md. Zahangir Asih, Devi Ratna Salleh, Md. Noor TP Chemical technology Immobilization of lipase enzyme produced from palm oil mill effluent (POME) by the activated carbon (AC) among the low cost support materials was optimized. The results indicated that immobilization of 94% was achieved by AC as the most suitable support material. A sequential optimization strategy based on a statistical experimental design, including one-factor-at-a-time (OFAT) method was used to determine the equilibrium time. Three components influencing lipase immobilization were optimized by the response surface methodology (RSM) based on the face-centered central composite design (FCCCD). On the statistical analysis of the results, the optimum enzyme concentration loading, agitation rate and carbon active dosage were found to be 30 U/ml, 300 rpm and 8 g/L respectively, with a maximum immobilization activity of 3732.9 U/gAC after 2 hrs of immobilization. Analysis of variance (ANOVA) showed a high regression coefficient (R 2) of 0.999, which indicated a satisfactory fit of the model with the experimental data. The parameters were statistically significant at p<0.05. World Academy of Science, Engineering and Technology 2014-06 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/40458/1/Paper-Canada.pdf Alam, Md. Zahangir and Asih, Devi Ratna and Salleh, Md. Noor (2014) Immobilization of lipase enzyme by low cost material: a statistical approach. In: International Science Conference, 16-17 June 2014, Toronto, Canada. http://www.internationalscienceconference.org |
| spellingShingle | TP Chemical technology Alam, Md. Zahangir Asih, Devi Ratna Salleh, Md. Noor Immobilization of lipase enzyme by low cost material: a statistical approach |
| title | Immobilization of lipase enzyme by low cost material: a statistical approach |
| title_full | Immobilization of lipase enzyme by low cost material: a statistical approach |
| title_fullStr | Immobilization of lipase enzyme by low cost material: a statistical approach |
| title_full_unstemmed | Immobilization of lipase enzyme by low cost material: a statistical approach |
| title_short | Immobilization of lipase enzyme by low cost material: a statistical approach |
| title_sort | immobilization of lipase enzyme by low cost material: a statistical approach |
| topic | TP Chemical technology |
| url | http://irep.iium.edu.my/40458/ http://irep.iium.edu.my/40458/ http://irep.iium.edu.my/40458/1/Paper-Canada.pdf |