A statistical framework for identifying past PRDM9 binding targets in primates

Recombination is a basic biological force, which, with mutation, plays an important role in generating new combinations of alleles in each chromosome, by shuffling and exchanging genetic material between maternal and paternal chromosomes. Multiple lines of evidence suggest that the rapidly evolving...

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Main Authors: Tumian, Afidalina, Myers, Simon
Format: Proceeding Paper
Language:English
English
Published: 2014
Subjects:
Online Access:http://irep.iium.edu.my/40420/
http://irep.iium.edu.my/40420/1/ICCCE14.afidalina.pdf
http://irep.iium.edu.my/40420/4/Sessions.pdf
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author Tumian, Afidalina
Myers, Simon
author_facet Tumian, Afidalina
Myers, Simon
author_sort Tumian, Afidalina
building IIUM Repository
collection Online Access
description Recombination is a basic biological force, which, with mutation, plays an important role in generating new combinations of alleles in each chromosome, by shuffling and exchanging genetic material between maternal and paternal chromosomes. Multiple lines of evidence suggest that the rapidly evolving zinc-finger (ZF) protein, PRDM9, is responsible for initiating much or all of recombination in human. PRDM9 shows extreme variation in both the number and sequence of its ZFs, between species and amongst individuals, across mammals. The rapid evolution of the PRDM9 ZF array may be a response to escape a self-destructive drive called biased gene conversion, which can cause preferential transmission of hotspot disrupting alleles, and leading to erosion of vital recombination sites – and hotspot signaling motifs – in the genome through time. This research attempts to uncover ancestral PRDM9 binding targets in humans and primates. By using six primate genomes, this work involves the development of statistical methods that identifies the locations where meiotic recombination could have occurred in the past. This is achieved by looking for short words that have undergone rapid losses or gains in each lineage. As a result, we found many short and different words across lineages. In conclusion, our findings imply a rapidly evolving mechanism landscape of past PRDM9 binding targets.
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English
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spelling iium-404202015-02-04T02:10:43Z http://irep.iium.edu.my/40420/ A statistical framework for identifying past PRDM9 binding targets in primates Tumian, Afidalina Myers, Simon Q Science (General) Recombination is a basic biological force, which, with mutation, plays an important role in generating new combinations of alleles in each chromosome, by shuffling and exchanging genetic material between maternal and paternal chromosomes. Multiple lines of evidence suggest that the rapidly evolving zinc-finger (ZF) protein, PRDM9, is responsible for initiating much or all of recombination in human. PRDM9 shows extreme variation in both the number and sequence of its ZFs, between species and amongst individuals, across mammals. The rapid evolution of the PRDM9 ZF array may be a response to escape a self-destructive drive called biased gene conversion, which can cause preferential transmission of hotspot disrupting alleles, and leading to erosion of vital recombination sites – and hotspot signaling motifs – in the genome through time. This research attempts to uncover ancestral PRDM9 binding targets in humans and primates. By using six primate genomes, this work involves the development of statistical methods that identifies the locations where meiotic recombination could have occurred in the past. This is achieved by looking for short words that have undergone rapid losses or gains in each lineage. As a result, we found many short and different words across lineages. In conclusion, our findings imply a rapidly evolving mechanism landscape of past PRDM9 binding targets. 2014 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/40420/1/ICCCE14.afidalina.pdf application/pdf en http://irep.iium.edu.my/40420/4/Sessions.pdf Tumian, Afidalina and Myers, Simon (2014) A statistical framework for identifying past PRDM9 binding targets in primates. In: International Conference on Computer & Communication Engineering 2014 (ICCCE 2014) , 23-25 September 2014, Kuala Lumpur. (Unpublished)
spellingShingle Q Science (General)
Tumian, Afidalina
Myers, Simon
A statistical framework for identifying past PRDM9 binding targets in primates
title A statistical framework for identifying past PRDM9 binding targets in primates
title_full A statistical framework for identifying past PRDM9 binding targets in primates
title_fullStr A statistical framework for identifying past PRDM9 binding targets in primates
title_full_unstemmed A statistical framework for identifying past PRDM9 binding targets in primates
title_short A statistical framework for identifying past PRDM9 binding targets in primates
title_sort statistical framework for identifying past prdm9 binding targets in primates
topic Q Science (General)
url http://irep.iium.edu.my/40420/
http://irep.iium.edu.my/40420/1/ICCCE14.afidalina.pdf
http://irep.iium.edu.my/40420/4/Sessions.pdf