Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres
In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2014
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| Online Access: | http://irep.iium.edu.my/39998/ http://irep.iium.edu.my/39998/1/39998.pdf |
| _version_ | 1848781881304154112 |
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| author | Rahman, Md. Mokhlesur Mohamed, Awang Haliza, Katas Ansary , Rezaul H. |
| author_facet | Rahman, Md. Mokhlesur Mohamed, Awang Haliza, Katas Ansary , Rezaul H. |
| author_sort | Rahman, Md. Mokhlesur |
| building | IIUM Repository |
| collection | Online Access |
| description | In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres were characterized with respect to their morphology, particle size, encapsulation efficiency, production yield, thermal properties and in vitro drug release. By using w/o/o/w method, a significant decrease in mean particle size and a significant increase in encapsulation efficiency were observed when a binary solvent mixture of ethyl acetate and dichloromethane was used. In w/o/o/w double emulsion solvent evaporation method, the optimized formulation of BSA loaded microspheres was nonporous, smooth-surfaced, and spherical shape under field-emission scanning electron microscope (FE-SEM) with a mean particle size of 3.95 µm and encapsulation efficiency of 98.46%. From 72 days In vitro release studies, microspheres prepared by modified (w/o/o/w) method with a combination of hydroxyl and carboxyl terminated PLGA polymers exhibited a significantly lower initial burst release followed by sustained and almost complete release compared to microspheres prepared by conventional w/o/w technique using a single PLGA polymer. It can be concluded that the modified w/o/o/w method can be proposed as a potential delivery system of therapeutic proteins. |
| first_indexed | 2025-11-14T15:56:36Z |
| format | Proceeding Paper |
| id | iium-39998 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:56:36Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-399982018-06-11T03:48:38Z http://irep.iium.edu.my/39998/ Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres Rahman, Md. Mokhlesur Mohamed, Awang Haliza, Katas Ansary , Rezaul H. QH301 Biology In this study, model protein bovine serum albumin (BSA) loaded poly(lactide-co-glycolide) (PLGA) microspheres have been prepared by a conventional water-in-oil-in-water (w/o/w) and a modified water-in-oil-in-oil-in-water (w/o/o/w) double emulsion solvent evaporation method. The prepared microspheres were characterized with respect to their morphology, particle size, encapsulation efficiency, production yield, thermal properties and in vitro drug release. By using w/o/o/w method, a significant decrease in mean particle size and a significant increase in encapsulation efficiency were observed when a binary solvent mixture of ethyl acetate and dichloromethane was used. In w/o/o/w double emulsion solvent evaporation method, the optimized formulation of BSA loaded microspheres was nonporous, smooth-surfaced, and spherical shape under field-emission scanning electron microscope (FE-SEM) with a mean particle size of 3.95 µm and encapsulation efficiency of 98.46%. From 72 days In vitro release studies, microspheres prepared by modified (w/o/o/w) method with a combination of hydroxyl and carboxyl terminated PLGA polymers exhibited a significantly lower initial burst release followed by sustained and almost complete release compared to microspheres prepared by conventional w/o/w technique using a single PLGA polymer. It can be concluded that the modified w/o/o/w method can be proposed as a potential delivery system of therapeutic proteins. 2014-10-28 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/39998/1/39998.pdf Rahman, Md. Mokhlesur and Mohamed, Awang and Haliza, Katas and Ansary , Rezaul H. (2014) Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres. In: Malaysia International Biological Symposium 2014 (i-SIMBIOMAS 2014), 28th - 29th October 2014, Putrajaya. http://www.science.upm.edu.my/isimbiomas2014/ |
| spellingShingle | QH301 Biology Rahman, Md. Mokhlesur Mohamed, Awang Haliza, Katas Ansary , Rezaul H. Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres |
| title | Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres |
| title_full | Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres |
| title_fullStr | Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres |
| title_full_unstemmed | Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres |
| title_short | Preparation, characterization and reduction of burst release of bovine serum albumin (BSA) from biodegradable PLGA microspheres |
| title_sort | preparation, characterization and reduction of burst release of bovine serum albumin (bsa) from biodegradable plga microspheres |
| topic | QH301 Biology |
| url | http://irep.iium.edu.my/39998/ http://irep.iium.edu.my/39998/ http://irep.iium.edu.my/39998/1/39998.pdf |