Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera
Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictu...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/39224/ http://irep.iium.edu.my/39224/1/39224.pdf |
| _version_ | 1848781743139586048 |
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| author | Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi |
| author_facet | Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi |
| author_sort | Mahmod, Safa Senan |
| building | IIUM Repository |
| collection | Online Access |
| description | Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictularus punctatus) and was immobilized based on cross-liked enzyme aggregate methodology. Glutaraldehyde was used as a cross-linker, acetone as precipitant and bovine serum albumin as an additive to improve the formation of CLEA-lipase. Experimental design was carried out by Response Surface Methodology using central composite design to find the optimum conditions for immobilization which was 50 mM of glutaraldehyde, 60% (v/v) Acetone and 0.11 mM of BSA. Finally, CLEA-lipase’s specific activity was 1.713 U/mg that is 20.43% higher than the free lipase activity. |
| first_indexed | 2025-11-14T15:54:25Z |
| format | Proceeding Paper |
| id | iium-39224 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:54:25Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-392242015-04-10T01:45:26Z http://irep.iium.edu.my/39224/ Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi TP248.13 Biotechnology Cross linked enzyme aggregates technology is one of the latest methods in immobilization of enzymes. This technique provides biocatalysts with higher activity, stability and reusability in industrial applications. In this research, lipase was extracted from the viscera of river channel catfish (Ictularus punctatus) and was immobilized based on cross-liked enzyme aggregate methodology. Glutaraldehyde was used as a cross-linker, acetone as precipitant and bovine serum albumin as an additive to improve the formation of CLEA-lipase. Experimental design was carried out by Response Surface Methodology using central composite design to find the optimum conditions for immobilization which was 50 mM of glutaraldehyde, 60% (v/v) Acetone and 0.11 mM of BSA. Finally, CLEA-lipase’s specific activity was 1.713 U/mg that is 20.43% higher than the free lipase activity. 2014 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/39224/1/39224.pdf Mahmod, Safa Senan and Yusof, Faridah and Jami, Mohammed Saedi (2014) Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera. In: International Conference on Biological, Chemical and Environmental Sciences (BCES-2014), 14-15 Jun 2014, Penang. http://iicbe.org/siteadmin/upload/7773C614017.pdf |
| spellingShingle | TP248.13 Biotechnology Mahmod, Safa Senan Yusof, Faridah Jami, Mohammed Saedi Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera |
| title | Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera |
| title_full | Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera |
| title_fullStr | Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera |
| title_full_unstemmed | Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera |
| title_short | Production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera |
| title_sort | production of cross-linked enzyme aggregate-lipase from channel catfish (ictularus punctatus) viscera |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/39224/ http://irep.iium.edu.my/39224/ http://irep.iium.edu.my/39224/1/39224.pdf |