Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharose™ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Centra...
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| Format: | Article |
| Language: | English English |
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AENSI Publications
2014
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| Online Access: | http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/1/P_46.pdf http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf |
| _version_ | 1848781742251442176 |
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| author | Mohamed, Nazhirah Yusof, Faridah |
| author_facet | Mohamed, Nazhirah Yusof, Faridah |
| author_sort | Mohamed, Nazhirah |
| building | IIUM Repository |
| collection | Online Access |
| description | A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharose™ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Central Composite Design (CCD) in Response Surface Methodology (RSM) were employed to optimize the protein buffer in order to improve purification. Design Expert® 6.0.8. software was applied using a 2-factor, 5-level to find the optimum pH and concentration of protein buffer to yield the highest lipase acyl hydrolase. In this study, it is concluded that purification at pH 8.0, 20 mM Tris-HCl buffer on ion exchange column chromatography results in the production of the highest total LAH activity (61.152 U).
The study has good significance in that it is an initial attempt to purify LAH enzyme, which has known as antifungal agent, from skim latex. |
| first_indexed | 2025-11-14T15:54:24Z |
| format | Article |
| id | iium-39221 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T15:54:24Z |
| publishDate | 2014 |
| publisher | AENSI Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-392212020-10-28T04:23:14Z http://irep.iium.edu.my/39221/ Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis Mohamed, Nazhirah Yusof, Faridah TP248.13 Biotechnology A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharose™ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Central Composite Design (CCD) in Response Surface Methodology (RSM) were employed to optimize the protein buffer in order to improve purification. Design Expert® 6.0.8. software was applied using a 2-factor, 5-level to find the optimum pH and concentration of protein buffer to yield the highest lipase acyl hydrolase. In this study, it is concluded that purification at pH 8.0, 20 mM Tris-HCl buffer on ion exchange column chromatography results in the production of the highest total LAH activity (61.152 U). The study has good significance in that it is an initial attempt to purify LAH enzyme, which has known as antifungal agent, from skim latex. AENSI Publications 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39221/1/P_46.pdf application/pdf en http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf Mohamed, Nazhirah and Yusof, Faridah (2014) Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis. Advances in Environmental Biology, 8 (3). pp. 672-679. ISSN 1995-0756 E-ISSN 1998-1066 http://www.aensiweb.com/old/aeb/2014/672-679.pdf |
| spellingShingle | TP248.13 Biotechnology Mohamed, Nazhirah Yusof, Faridah Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis |
| title | Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis |
| title_full | Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis |
| title_fullStr | Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis |
| title_full_unstemmed | Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis |
| title_short | Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis |
| title_sort | experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/1/P_46.pdf http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf |