Biotransformation of ω fatty acids using pseudomonas aeruginosa
Biotransformation of fish oil containing ω fatty acids using Pseudomonas spp has been carried out. In the present study, Pseudomonas aeruginosa was used to transform ω fatty acids, extracted from Malaysian catfish Clarias batrachus, into useful metabolite. The fatty acids were extracted from the f...
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| Format: | Proceeding Paper |
| Language: | English English English English |
| Published: |
2012
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| Online Access: | http://irep.iium.edu.my/39021/ http://irep.iium.edu.my/39021/1/IBS_2012_Poster.pdf http://irep.iium.edu.my/39021/3/IBS__2012_Poster_Biotransformation_of_%CF%89_fatty_acids_using_Pseudomonas_aeruginosa.pdf http://irep.iium.edu.my/39021/2/DR_NO_Abstract_IBS_2012.pdf http://irep.iium.edu.my/39021/9/IBS2012-2.pdf |
| _version_ | 1848781709249609728 |
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| author | Omar, Muhammad Nor |
| author_facet | Omar, Muhammad Nor |
| author_sort | Omar, Muhammad Nor |
| building | IIUM Repository |
| collection | Online Access |
| description | Biotransformation of fish oil containing ω fatty acids using Pseudomonas spp has been carried out. In the
present study, Pseudomonas aeruginosa was used to transform ω fatty acids, extracted from Malaysian
catfish Clarias batrachus, into useful metabolite. The fatty acids were extracted from the freeze-dried
catfish flesh using a modified Folch method with the chloroform and methanol system. The crude oil was
added to the bacterial culture and incubated at 28oC for 4 days. After incubation, the biotransformed
product was extracted and analyzed by using gas chromatography and mass spectrometer (GC-MS) to
identify the fatty acids and other compounds. It was found that several fatty acids, especially ω-fatty acids
were converted to hydroxyl fatty acids. This indicates that ω-fatty acids can be used as starting materials
for other bioactive metabolites for pharmaceutical purposes. |
| first_indexed | 2025-11-14T15:53:52Z |
| format | Proceeding Paper |
| id | iium-39021 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English English |
| last_indexed | 2025-11-14T15:53:52Z |
| publishDate | 2012 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-390212014-11-06T08:20:08Z http://irep.iium.edu.my/39021/ Biotransformation of ω fatty acids using pseudomonas aeruginosa Omar, Muhammad Nor QD Chemistry Biotransformation of fish oil containing ω fatty acids using Pseudomonas spp has been carried out. In the present study, Pseudomonas aeruginosa was used to transform ω fatty acids, extracted from Malaysian catfish Clarias batrachus, into useful metabolite. The fatty acids were extracted from the freeze-dried catfish flesh using a modified Folch method with the chloroform and methanol system. The crude oil was added to the bacterial culture and incubated at 28oC for 4 days. After incubation, the biotransformed product was extracted and analyzed by using gas chromatography and mass spectrometer (GC-MS) to identify the fatty acids and other compounds. It was found that several fatty acids, especially ω-fatty acids were converted to hydroxyl fatty acids. This indicates that ω-fatty acids can be used as starting materials for other bioactive metabolites for pharmaceutical purposes. 2012-09 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/39021/1/IBS_2012_Poster.pdf application/pdf en http://irep.iium.edu.my/39021/3/IBS__2012_Poster_Biotransformation_of_%CF%89_fatty_acids_using_Pseudomonas_aeruginosa.pdf application/pdf en http://irep.iium.edu.my/39021/2/DR_NO_Abstract_IBS_2012.pdf application/pdf en http://irep.iium.edu.my/39021/9/IBS2012-2.pdf Omar, Muhammad Nor (2012) Biotransformation of ω fatty acids using pseudomonas aeruginosa. In: International Biotechnology Symposium and Exhibition (IBS 2012), 16-21 September 2012, Daegu, Korea. (Unpublished) |
| spellingShingle | QD Chemistry Omar, Muhammad Nor Biotransformation of ω fatty acids using pseudomonas aeruginosa |
| title | Biotransformation of ω fatty acids using pseudomonas aeruginosa |
| title_full | Biotransformation of ω fatty acids using pseudomonas aeruginosa |
| title_fullStr | Biotransformation of ω fatty acids using pseudomonas aeruginosa |
| title_full_unstemmed | Biotransformation of ω fatty acids using pseudomonas aeruginosa |
| title_short | Biotransformation of ω fatty acids using pseudomonas aeruginosa |
| title_sort | biotransformation of ω fatty acids using pseudomonas aeruginosa |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/39021/ http://irep.iium.edu.my/39021/1/IBS_2012_Poster.pdf http://irep.iium.edu.my/39021/3/IBS__2012_Poster_Biotransformation_of_%CF%89_fatty_acids_using_Pseudomonas_aeruginosa.pdf http://irep.iium.edu.my/39021/2/DR_NO_Abstract_IBS_2012.pdf http://irep.iium.edu.my/39021/9/IBS2012-2.pdf |