Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains
IBDV outbreak had been reported to infect broiler chicken in Malaysia and cause high mortality. It had been reported that the virulence and pathogenicity of the virus is related to its coated protein, specifically the VP2 hypervariable regions. To study this, the nucleotides and protein of the loca...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Scholars Research Library
2013
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/38464/ http://irep.iium.edu.my/38464/1/ABR-2013-4-5-92-104.pdf |
| _version_ | 1848781612205998080 |
|---|---|
| author | Ghazali, Mohd. Shahrin Ahmad Raus, Raha Mat Amin, Noriha Shohaimi, Syamsiah Aini Abu Bakar, Farah Diba Zainuddin, Norafiza Nor Muhammad, Nor Azlin Alia |
| author_facet | Ghazali, Mohd. Shahrin Ahmad Raus, Raha Mat Amin, Noriha Shohaimi, Syamsiah Aini Abu Bakar, Farah Diba Zainuddin, Norafiza Nor Muhammad, Nor Azlin Alia |
| author_sort | Ghazali, Mohd. Shahrin |
| building | IIUM Repository |
| collection | Online Access |
| description | IBDV outbreak had been reported to infect broiler chicken in Malaysia and cause high mortality. It had been
reported that the virulence and pathogenicity of the virus is related to its coated protein, specifically the VP2 hypervariable regions. To study this, the nucleotides and protein of the local IBDV isolate was sequenced and
analyzed by comparing it with other IBDV that had been reported previously. To obtain the virus sequence, local
IBDV isolate strain 3529/92 was propagated in SPF eggs and RNA were extracted, amplify by rt (reverse transcriptase)-PCR before being inserted into pCR 2.1 TOPO for cloning and sequencing. The full length of the
gene segment for the coat protein was constructed by concomitantly joining the fragments using the native
restrictions sites of IBDV in pTrcHis2a expression vector. The sequence analysis revealed that the local IBDV strain 3529/92 consists of 3039 nucleotides which encodes for 1012 amino acids. BLAST analysis of the nucleotides sequence showed that the local strain shared greatest similarity with the Dutch D6948 vv IBDV subtypes. Analysis on the VP2 variable regions of 3529/92 showed most amino acid substitutions at VP2 variables regions are similar to the changes in VP2 variable regions of vvIBDV subtypes. Phylogenetic analysis prove that that 3529/92 isolates closely related with vvIBDV subtypes. The nucleotides sequences of ORF A2 3529/92 will give great insight in vaccine development against IBDV especially vvIBDV subtypes. |
| first_indexed | 2025-11-14T15:52:20Z |
| format | Article |
| id | iium-38464 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:52:20Z |
| publishDate | 2013 |
| publisher | Scholars Research Library |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-384642024-06-19T04:17:43Z http://irep.iium.edu.my/38464/ Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains Ghazali, Mohd. Shahrin Ahmad Raus, Raha Mat Amin, Noriha Shohaimi, Syamsiah Aini Abu Bakar, Farah Diba Zainuddin, Norafiza Nor Muhammad, Nor Azlin Alia QH426 Genetics IBDV outbreak had been reported to infect broiler chicken in Malaysia and cause high mortality. It had been reported that the virulence and pathogenicity of the virus is related to its coated protein, specifically the VP2 hypervariable regions. To study this, the nucleotides and protein of the local IBDV isolate was sequenced and analyzed by comparing it with other IBDV that had been reported previously. To obtain the virus sequence, local IBDV isolate strain 3529/92 was propagated in SPF eggs and RNA were extracted, amplify by rt (reverse transcriptase)-PCR before being inserted into pCR 2.1 TOPO for cloning and sequencing. The full length of the gene segment for the coat protein was constructed by concomitantly joining the fragments using the native restrictions sites of IBDV in pTrcHis2a expression vector. The sequence analysis revealed that the local IBDV strain 3529/92 consists of 3039 nucleotides which encodes for 1012 amino acids. BLAST analysis of the nucleotides sequence showed that the local strain shared greatest similarity with the Dutch D6948 vv IBDV subtypes. Analysis on the VP2 variable regions of 3529/92 showed most amino acid substitutions at VP2 variables regions are similar to the changes in VP2 variable regions of vvIBDV subtypes. Phylogenetic analysis prove that that 3529/92 isolates closely related with vvIBDV subtypes. The nucleotides sequences of ORF A2 3529/92 will give great insight in vaccine development against IBDV especially vvIBDV subtypes. Scholars Research Library 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38464/1/ABR-2013-4-5-92-104.pdf Ghazali, Mohd. Shahrin and Ahmad Raus, Raha and Mat Amin, Noriha and Shohaimi, Syamsiah Aini and Abu Bakar, Farah Diba and Zainuddin, Norafiza and Nor Muhammad, Nor Azlin Alia (2013) Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains. Annals of Biological Research, 4 (5). pp. 92-104. ISSN 0976-1233 http://scholarsresearchlibrary.com/ABR-vol4-iss5/ABR-2013-4-5-92-104.pdf |
| spellingShingle | QH426 Genetics Ghazali, Mohd. Shahrin Ahmad Raus, Raha Mat Amin, Noriha Shohaimi, Syamsiah Aini Abu Bakar, Farah Diba Zainuddin, Norafiza Nor Muhammad, Nor Azlin Alia Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains |
| title | Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains |
| title_full | Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains |
| title_fullStr | Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains |
| title_full_unstemmed | Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains |
| title_short | Genomic analysis and comparison of very virulent infectious bursal disease virus (vvIBDV) affecting Malaysian poultry chickens with other IBDV strains |
| title_sort | genomic analysis and comparison of very virulent infectious bursal disease virus (vvibdv) affecting malaysian poultry chickens with other ibdv strains |
| topic | QH426 Genetics |
| url | http://irep.iium.edu.my/38464/ http://irep.iium.edu.my/38464/ http://irep.iium.edu.my/38464/1/ABR-2013-4-5-92-104.pdf |