Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design
Production of bioethanol from oil palm empty fruit bunches (EFB) by Saccharomyces cerevisae and Aspergillus niger is among the ways of reducing environmental pollution and consumption of crude oil. This study used sequential optimization approach based on statistical experimental design including Pl...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2014
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| Online Access: | http://irep.iium.edu.my/38271/ http://irep.iium.edu.my/38271/3/JPAM-zahangir.pdf |
| _version_ | 1848781574490816512 |
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| author | Alam, Md Zahangir Zainan, Nur Hidayah Al-Khatib, Ma'an Fahmi Rashid |
| author_facet | Alam, Md Zahangir Zainan, Nur Hidayah Al-Khatib, Ma'an Fahmi Rashid |
| author_sort | Alam, Md Zahangir |
| building | IIUM Repository |
| collection | Online Access |
| description | Production of bioethanol from oil palm empty fruit bunches (EFB) by Saccharomyces cerevisae and Aspergillus niger is among the ways of reducing environmental pollution and consumption of crude oil. This study used sequential optimization approach based on statistical experimental design including Plackett-Burman (PB) design, one-factor-at-a-time (OFAT) and face-centered central composite design (FCCCD). Among the parameters tested, pH, temperature, inoculum size, potassium dihydrogen phosphate (KH2PO4), magnesium sulphate heptahydrate (MgSO4.7H2O) and peptone showed positive effects while yeast extract, malt extract, potassium chloride, urea and agitation were influencing the production negatively. The three parameters chosen for determination of the optimum values by response surface methodology (RSM) based on the FCCCD were pH, KH2PO4 and agitation. Although agitation showed negative effects it was considered in FCCCD due to the mixing effect of fermnetation. The validity of the model was verified and the optimum value of pH 5.5, agitation of 150rpm and 0.3% of KH2PO4 led to a maximum bioethanol production of 7.4 g/l. The yield of bioethanol was determined based on the reducing sugar (16.85 g/l) obtained from the EFB. |
| first_indexed | 2025-11-14T15:51:44Z |
| format | Article |
| id | iium-38271 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:51:44Z |
| publishDate | 2014 |
| publisher | Journal of Pure and Applied Microbiology |
| recordtype | eprints |
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| spelling | iium-382712024-05-02T04:35:47Z http://irep.iium.edu.my/38271/ Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design Alam, Md Zahangir Zainan, Nur Hidayah Al-Khatib, Ma'an Fahmi Rashid TP315 Fuel Production of bioethanol from oil palm empty fruit bunches (EFB) by Saccharomyces cerevisae and Aspergillus niger is among the ways of reducing environmental pollution and consumption of crude oil. This study used sequential optimization approach based on statistical experimental design including Plackett-Burman (PB) design, one-factor-at-a-time (OFAT) and face-centered central composite design (FCCCD). Among the parameters tested, pH, temperature, inoculum size, potassium dihydrogen phosphate (KH2PO4), magnesium sulphate heptahydrate (MgSO4.7H2O) and peptone showed positive effects while yeast extract, malt extract, potassium chloride, urea and agitation were influencing the production negatively. The three parameters chosen for determination of the optimum values by response surface methodology (RSM) based on the FCCCD were pH, KH2PO4 and agitation. Although agitation showed negative effects it was considered in FCCCD due to the mixing effect of fermnetation. The validity of the model was verified and the optimum value of pH 5.5, agitation of 150rpm and 0.3% of KH2PO4 led to a maximum bioethanol production of 7.4 g/l. The yield of bioethanol was determined based on the reducing sugar (16.85 g/l) obtained from the EFB. Journal of Pure and Applied Microbiology 2014-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38271/3/JPAM-zahangir.pdf Alam, Md Zahangir and Zainan, Nur Hidayah and Al-Khatib, Ma'an Fahmi Rashid (2014) Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design. Journal of Pure and Applied Microbiology, 8 (s.ed 1). pp. 731-740. ISSN 0973-7510 http://www.microbiologyjournal.org/ |
| spellingShingle | TP315 Fuel Alam, Md Zahangir Zainan, Nur Hidayah Al-Khatib, Ma'an Fahmi Rashid Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design |
| title | Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design |
| title_full | Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design |
| title_fullStr | Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design |
| title_full_unstemmed | Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design |
| title_short | Optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design |
| title_sort | optimization of bioethanol production from empty fruit bunches by co-culture of saccharomyces cerevisae and aspergillus niger using statistical experimental design |
| topic | TP315 Fuel |
| url | http://irep.iium.edu.my/38271/ http://irep.iium.edu.my/38271/ http://irep.iium.edu.my/38271/3/JPAM-zahangir.pdf |