In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts
The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC)...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier Ltd.
2010
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf |
| _version_ | 1848781567918342144 |
|---|---|
| author | Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum |
| author_facet | Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum |
| author_sort | Abdull Rasad, Mohammad Syaiful Bahari |
| building | IIUM Repository |
| collection | Online Access |
| description | The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible
utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan
(O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC) derivatives
in sheet like and paste forms were evaluated using primary normal human dermal fibroblast
cultures and hypertrophic scars; a fibrotic conditions representing a model of altered wound healing with
overproduction of extracellular matrix and fibroblast hyperproliferative activity. Cytotoxicities of these
chitosan derivatives were assessed using 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide
(MTT) assay. The results indicate that both chitosan derivative sheets and pastes have appropriate cytocompatibility
and appear promising as safe biomaterials with potential wound healing applications. The
SH120 (NO-CMC) derivatives sheet exhibited highest cytocompatibility property and may be regulated
by MMP-13 in controlling the cell growth and its expression level. |
| first_indexed | 2025-11-14T15:51:37Z |
| format | Article |
| id | iium-38238 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T15:51:37Z |
| publishDate | 2010 |
| publisher | Elsevier Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-382382022-06-09T01:41:34Z http://irep.iium.edu.my/38238/ In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum Q Science (General) The cytocompatibility of novel chitosan derivative sheets and pastes were evaluated in vitro for possible utilization in wound dressing applications for wound healing. In this study, the cytotoxicity of oligochitosan (O-C), N,O-carboxymethyl-chitosan (NO-CMC) and N- carboxymethyl-chitosan (N-CMC) derivatives in sheet like and paste forms were evaluated using primary normal human dermal fibroblast cultures and hypertrophic scars; a fibrotic conditions representing a model of altered wound healing with overproduction of extracellular matrix and fibroblast hyperproliferative activity. Cytotoxicities of these chitosan derivatives were assessed using 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The results indicate that both chitosan derivative sheets and pastes have appropriate cytocompatibility and appear promising as safe biomaterials with potential wound healing applications. The SH120 (NO-CMC) derivatives sheet exhibited highest cytocompatibility property and may be regulated by MMP-13 in controlling the cell growth and its expression level. Elsevier Ltd. 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf Abdull Rasad, Mohammad Syaiful Bahari and Halim, Ahmad Sukari and Hashim, Kamaruddin and Abdul Rashid , Ahmad Hazri and Yusof, Norimah and Shamsuddin, Shaharum (2010) In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts. Carbohydrate Polymers, 79. pp. 1094-1100. ISSN 0144-8617 (O) http://www.journals.elsevier.com/carbohydrate-polymers 10.1016/j.carbpol.2009.10.048 |
| spellingShingle | Q Science (General) Abdull Rasad, Mohammad Syaiful Bahari Halim, Ahmad Sukari Hashim, Kamaruddin Abdul Rashid , Ahmad Hazri Yusof, Norimah Shamsuddin, Shaharum In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts |
| title | In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts |
| title_full | In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts |
| title_fullStr | In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts |
| title_full_unstemmed | In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts |
| title_short | In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts |
| title_sort | in vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/ http://irep.iium.edu.my/38238/1/Carbohydrate_Polymers_Journal.pdf |