Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials
A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volu...
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| Format: | Article |
| Language: | English English |
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Japan Oil Chemists' Society
2014
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| Online Access: | http://irep.iium.edu.my/38191/ http://irep.iium.edu.my/38191/1/Paper-JOS-devi.pdf http://irep.iium.edu.my/38191/4/38191-Pilot-scale%20production%20of%20lipase%20using%20palm%20oil%20mill%20effluent%20as%20a%20basal%20medium_SCOPUS.pdf |
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| author | Asih, Devi Ratna Alam, Md Zahangir Salleh, Md. Noor Salihu, Aliyu |
| author_facet | Asih, Devi Ratna Alam, Md Zahangir Salleh, Md. Noor Salihu, Aliyu |
| author_sort | Asih, Devi Ratna |
| building | IIUM Repository |
| collection | Online Access |
| description | A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volume (P/V) and constant tip speed. Moreover, lipase immobilization on different materials was also investigated. Lipase production was performed using liquid-state bioconversion of POME as the medium and Candida cylindracea as the inoculum. The fermentation medium was composed of 1% total suspended solids (TSS) of POME, 0.5% (w/v) peptone, 0.7% (v/v) Tween-80, and 2.2% inoculum. The medium composition was decided on the basis of the medium optimization results of a previous study. The fermentation was carried out for 48 h at 30°C and pH 6. The maximum lipase production was 5.72U/mL and 21.34 U/mL, obtained from the scale-up strategies of constant tip speed and P/V, respectively. Four accessible support materials were screened for their potential use in immobilization. The most suitable support material was found to be activated carbon, with a maximum immobilization of 94%. |
| first_indexed | 2025-11-14T15:51:28Z |
| format | Article |
| id | iium-38191 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T15:51:28Z |
| publishDate | 2014 |
| publisher | Japan Oil Chemists' Society |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-381912017-08-10T07:21:51Z http://irep.iium.edu.my/38191/ Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials Asih, Devi Ratna Alam, Md Zahangir Salleh, Md. Noor Salihu, Aliyu TP248.13 Biotechnology A pilot-scale production of lipase using palm oil mill effluent (POME) as a fermentation basal medium was carried out, and parameters for immobilization of the produced lipase were optimized. Lipase production in a 300-L bioreactor was performed using two proposed strategies, constant power per volume (P/V) and constant tip speed. Moreover, lipase immobilization on different materials was also investigated. Lipase production was performed using liquid-state bioconversion of POME as the medium and Candida cylindracea as the inoculum. The fermentation medium was composed of 1% total suspended solids (TSS) of POME, 0.5% (w/v) peptone, 0.7% (v/v) Tween-80, and 2.2% inoculum. The medium composition was decided on the basis of the medium optimization results of a previous study. The fermentation was carried out for 48 h at 30°C and pH 6. The maximum lipase production was 5.72U/mL and 21.34 U/mL, obtained from the scale-up strategies of constant tip speed and P/V, respectively. Four accessible support materials were screened for their potential use in immobilization. The most suitable support material was found to be activated carbon, with a maximum immobilization of 94%. Japan Oil Chemists' Society 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38191/1/Paper-JOS-devi.pdf application/pdf en http://irep.iium.edu.my/38191/4/38191-Pilot-scale%20production%20of%20lipase%20using%20palm%20oil%20mill%20effluent%20as%20a%20basal%20medium_SCOPUS.pdf Asih, Devi Ratna and Alam, Md Zahangir and Salleh, Md. Noor and Salihu, Aliyu (2014) Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials. Journal of Oleo Science, 63 (8). pp. 779-785. ISSN 1345-8957 E-ISSN 1347-3352 http://dx.doi.org/10.5650/jos.ess13187 doi:10.5650/jos.ess13187 |
| spellingShingle | TP248.13 Biotechnology Asih, Devi Ratna Alam, Md Zahangir Salleh, Md. Noor Salihu, Aliyu Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials |
| title | Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials |
| title_full | Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials |
| title_fullStr | Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials |
| title_full_unstemmed | Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials |
| title_short | Pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials |
| title_sort | pilot-scale production of lipase using palm oil mill effluent as a basal medium and its immobilization by selected materials |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/38191/ http://irep.iium.edu.my/38191/ http://irep.iium.edu.my/38191/ http://irep.iium.edu.my/38191/1/Paper-JOS-devi.pdf http://irep.iium.edu.my/38191/4/38191-Pilot-scale%20production%20of%20lipase%20using%20palm%20oil%20mill%20effluent%20as%20a%20basal%20medium_SCOPUS.pdf |