Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms
Kraits (Bungarus spp.) are highly venomous elapids that are only found in Asia. In the current study, 103 and 86 different proteins were identified from Bungarus candidus and Bungarus fasciatus venoms, respectively. These proteins were classified into 18 different venom protein families. Both venoms...
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| Format: | Article |
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Elsevier Ltd.
2014
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| Online Access: | http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/1/38142_Proteomic%20characterization%20and%20comparison.pdf http://irep.iium.edu.my/38142/2/38142_Proteomic%20characterization%20and%20comparison_SCOPUS.pdf http://irep.iium.edu.my/38142/3/38142_Proteomic%20characterization%20and%20comparison_WOS.pdf |
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| author | Ahmad Rusmili, Muhamad Rusdi Ting, Tee Yee Mustafa, Mohd Rais Hodgson, Wayne C. Othman, Iekhsan |
| author_facet | Ahmad Rusmili, Muhamad Rusdi Ting, Tee Yee Mustafa, Mohd Rais Hodgson, Wayne C. Othman, Iekhsan |
| author_sort | Ahmad Rusmili, Muhamad Rusdi |
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| collection | Online Access |
| description | Kraits (Bungarus spp.) are highly venomous elapids that are only found in Asia. In the current study, 103 and 86 different proteins were identified from Bungarus candidus and Bungarus fasciatus venoms, respectively. These proteins were classified into 18 different venom protein families. Both venoms were found to contain a high percentage of three finger toxins, phospholipase A2 enzymes and Kunitz-type inhibitors. Smaller number of high molecular weight enzymes such as L-amino acid oxidase, hyaluronidases, and acetylcholinesterase were also detected in the venoms. We also detected some unique proteins that were not known to be present in these venoms. The presence of a natriuretic peptide, vespryn, and serine protease families was detected in B. candidus venom. We also detected the presence of subunit A and B of β-bungarotoxin and α-bungarotoxin which had not been previously found in B. fasciatus venom. Understanding the proteome composition of Malaysian krait species will provide useful information on unique toxins and proteins which are present in the venoms. This knowledge will assist in the management of krait envenoming. In addition, these proteins may have potential use as research tools or as drug-design templates. |
| first_indexed | 2025-11-14T15:51:17Z |
| format | Article |
| id | iium-38142 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T15:51:17Z |
| publishDate | 2014 |
| publisher | Elsevier Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-381422017-09-20T08:08:10Z http://irep.iium.edu.my/38142/ Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms Ahmad Rusmili, Muhamad Rusdi Ting, Tee Yee Mustafa, Mohd Rais Hodgson, Wayne C. Othman, Iekhsan QD Chemistry Kraits (Bungarus spp.) are highly venomous elapids that are only found in Asia. In the current study, 103 and 86 different proteins were identified from Bungarus candidus and Bungarus fasciatus venoms, respectively. These proteins were classified into 18 different venom protein families. Both venoms were found to contain a high percentage of three finger toxins, phospholipase A2 enzymes and Kunitz-type inhibitors. Smaller number of high molecular weight enzymes such as L-amino acid oxidase, hyaluronidases, and acetylcholinesterase were also detected in the venoms. We also detected some unique proteins that were not known to be present in these venoms. The presence of a natriuretic peptide, vespryn, and serine protease families was detected in B. candidus venom. We also detected the presence of subunit A and B of β-bungarotoxin and α-bungarotoxin which had not been previously found in B. fasciatus venom. Understanding the proteome composition of Malaysian krait species will provide useful information on unique toxins and proteins which are present in the venoms. This knowledge will assist in the management of krait envenoming. In addition, these proteins may have potential use as research tools or as drug-design templates. Elsevier Ltd. 2014-10-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38142/1/38142_Proteomic%20characterization%20and%20comparison.pdf application/pdf en http://irep.iium.edu.my/38142/2/38142_Proteomic%20characterization%20and%20comparison_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/38142/3/38142_Proteomic%20characterization%20and%20comparison_WOS.pdf Ahmad Rusmili, Muhamad Rusdi and Ting, Tee Yee and Mustafa, Mohd Rais and Hodgson, Wayne C. and Othman, Iekhsan (2014) Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms. Journal of Proteomics, 110. pp. 129-144. ISSN 1874-3919 http://www.sciencedirect.com/science/article/pii/S1874391914003996 10.1016/j.jprot.2014.08.001 |
| spellingShingle | QD Chemistry Ahmad Rusmili, Muhamad Rusdi Ting, Tee Yee Mustafa, Mohd Rais Hodgson, Wayne C. Othman, Iekhsan Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms |
| title | Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms |
| title_full | Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms |
| title_fullStr | Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms |
| title_full_unstemmed | Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms |
| title_short | Proteomic characterization and comparison of Malaysian Bungarus candidus and Bungarus fasciatus venoms |
| title_sort | proteomic characterization and comparison of malaysian bungarus candidus and bungarus fasciatus venoms |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/ http://irep.iium.edu.my/38142/1/38142_Proteomic%20characterization%20and%20comparison.pdf http://irep.iium.edu.my/38142/2/38142_Proteomic%20characterization%20and%20comparison_SCOPUS.pdf http://irep.iium.edu.my/38142/3/38142_Proteomic%20characterization%20and%20comparison_WOS.pdf |