Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask
An effective mammalian cell culture based host system is highly desirable for production of bio-therapeutics. There are several strategies to achieve an effective system, one of which is regulation of media coupled with exploitation of inherent characteristics of cell line. In this present study, i...
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| Format: | Article |
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American-Eurasian Network for Scientific Information
2014
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| Online Access: | http://irep.iium.edu.my/38024/ http://irep.iium.edu.my/38024/1/Paper_-_Packeer-Mohamed_et_al_2014_%28AEB%29.pdf http://irep.iium.edu.my/38024/4/38024_Effects%20of%20glutamine%20and%20serum_scopus.pdf |
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| author | Mohamed, Vasila Packeer Hashim, Yumi Zuhanis Has-Yun Mohmad Saberi, Salfarin Ezrina Ibrahim, Siti Nuramalis Amid, Azura Mel, Maizirwan |
| author_facet | Mohamed, Vasila Packeer Hashim, Yumi Zuhanis Has-Yun Mohmad Saberi, Salfarin Ezrina Ibrahim, Siti Nuramalis Amid, Azura Mel, Maizirwan |
| author_sort | Mohamed, Vasila Packeer |
| building | IIUM Repository |
| collection | Online Access |
| description | An effective mammalian cell culture based host system is highly desirable for production of bio-therapeutics. There are several strategies to achieve an effective
system, one of which is regulation of media coupled with exploitation of inherent characteristics of cell line. In this present study, it is hypothesized that glutamine and
serum may affect CHO-K1 cell growth through Insulin-like Growth Factor-I (IGF-I)pathway. The effects of glutamine and serum on IGF-dependent CHO-K1 cell growth in T-flask were studied based on three-level Full Factorial Design generated by SIMCAP+v12 (Umetrics, Sweden). The combination factor of 0.50 mM glutamine and 10 % (v/v) serum was found to be the optimal condition producing 8.87 x 105 cells/ml. The CHO-K1 culture was also found to be able to grow in zero glutamine reaching 16.60 x 105 cells/ ml. Meanwhile, improvement of cell growth can also be achieved in reduced serum level with the presence of IGF-I protein. |
| first_indexed | 2025-11-14T15:50:54Z |
| format | Article |
| id | iium-38024 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T15:50:54Z |
| publishDate | 2014 |
| publisher | American-Eurasian Network for Scientific Information |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-380242017-09-19T06:41:47Z http://irep.iium.edu.my/38024/ Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask Mohamed, Vasila Packeer Hashim, Yumi Zuhanis Has-Yun Mohmad Saberi, Salfarin Ezrina Ibrahim, Siti Nuramalis Amid, Azura Mel, Maizirwan Q Science (General) An effective mammalian cell culture based host system is highly desirable for production of bio-therapeutics. There are several strategies to achieve an effective system, one of which is regulation of media coupled with exploitation of inherent characteristics of cell line. In this present study, it is hypothesized that glutamine and serum may affect CHO-K1 cell growth through Insulin-like Growth Factor-I (IGF-I)pathway. The effects of glutamine and serum on IGF-dependent CHO-K1 cell growth in T-flask were studied based on three-level Full Factorial Design generated by SIMCAP+v12 (Umetrics, Sweden). The combination factor of 0.50 mM glutamine and 10 % (v/v) serum was found to be the optimal condition producing 8.87 x 105 cells/ml. The CHO-K1 culture was also found to be able to grow in zero glutamine reaching 16.60 x 105 cells/ ml. Meanwhile, improvement of cell growth can also be achieved in reduced serum level with the presence of IGF-I protein. American-Eurasian Network for Scientific Information 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/38024/1/Paper_-_Packeer-Mohamed_et_al_2014_%28AEB%29.pdf application/pdf en http://irep.iium.edu.my/38024/4/38024_Effects%20of%20glutamine%20and%20serum_scopus.pdf Mohamed, Vasila Packeer and Hashim, Yumi Zuhanis Has-Yun and Mohmad Saberi, Salfarin Ezrina and Ibrahim, Siti Nuramalis and Amid, Azura and Mel, Maizirwan (2014) Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask. Advances in Environmental Biology, 8 (3 ( Special Issue )). pp. 831-837. ISSN 1995-0756 E-ISSN 1998-1066 http://www.aensiweb.com/old/aeb/2014/831-837.pdf |
| spellingShingle | Q Science (General) Mohamed, Vasila Packeer Hashim, Yumi Zuhanis Has-Yun Mohmad Saberi, Salfarin Ezrina Ibrahim, Siti Nuramalis Amid, Azura Mel, Maizirwan Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask |
| title | Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask |
| title_full | Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask |
| title_fullStr | Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask |
| title_full_unstemmed | Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask |
| title_short | Effects of glutamine and serum on IGF- dependent cho-K1 cell growth in T-Flask |
| title_sort | effects of glutamine and serum on igf- dependent cho-k1 cell growth in t-flask |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/38024/ http://irep.iium.edu.my/38024/ http://irep.iium.edu.my/38024/1/Paper_-_Packeer-Mohamed_et_al_2014_%28AEB%29.pdf http://irep.iium.edu.my/38024/4/38024_Effects%20of%20glutamine%20and%20serum_scopus.pdf |