Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of nelumbo nucifera Gaertn. (Lotus), an aquatic edible plant
In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new char...
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| Format: | Article |
| Language: | English English |
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Hindawi Publishing Corporation
2014
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| Online Access: | http://irep.iium.edu.my/37971/ http://irep.iium.edu.my/37971/1/SWJ.pdf http://irep.iium.edu.my/37971/4/37971_Effects%20of%20NAA%20and%20BAP_SCOPUS.pdf |
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| author | Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Saleh, Azani Hasbullah, Nor Azlina Elias, Hashimah |
| author_facet | Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Saleh, Azani Hasbullah, Nor Azlina Elias, Hashimah |
| author_sort | Mahmad, Noraini |
| building | IIUM Repository |
| collection | Online Access |
| description | In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions. |
| first_indexed | 2025-11-14T15:50:42Z |
| format | Article |
| id | iium-37971 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T15:50:42Z |
| publishDate | 2014 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-379712021-07-23T16:11:10Z http://irep.iium.edu.my/37971/ Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of nelumbo nucifera Gaertn. (Lotus), an aquatic edible plant Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Saleh, Azani Hasbullah, Nor Azlina Elias, Hashimah S Agriculture (General) SB Plant culture TP248.13 Biotechnology In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2 : 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/37971/1/SWJ.pdf application/pdf en http://irep.iium.edu.my/37971/4/37971_Effects%20of%20NAA%20and%20BAP_SCOPUS.pdf Mahmad, Noraini and Mat Taha, Rosna and Othman, Rashidi and Saleh, Azani and Hasbullah, Nor Azlina and Elias, Hashimah (2014) Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of nelumbo nucifera Gaertn. (Lotus), an aquatic edible plant. The Scientific World Journal, 2014 (745148). pp. 1-8. ISSN 1537-744X http://www.hindawi.com/journals/tswj/2014/745148/ 10.1155/2014/745148 |
| spellingShingle | S Agriculture (General) SB Plant culture TP248.13 Biotechnology Mahmad, Noraini Mat Taha, Rosna Othman, Rashidi Saleh, Azani Hasbullah, Nor Azlina Elias, Hashimah Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of nelumbo nucifera Gaertn. (Lotus), an aquatic edible plant |
| title | Effects of NAA and BAP, double-layered media, and
light distance on in vitro regeneration of nelumbo nucifera
Gaertn. (Lotus), an aquatic edible plant |
| title_full | Effects of NAA and BAP, double-layered media, and
light distance on in vitro regeneration of nelumbo nucifera
Gaertn. (Lotus), an aquatic edible plant |
| title_fullStr | Effects of NAA and BAP, double-layered media, and
light distance on in vitro regeneration of nelumbo nucifera
Gaertn. (Lotus), an aquatic edible plant |
| title_full_unstemmed | Effects of NAA and BAP, double-layered media, and
light distance on in vitro regeneration of nelumbo nucifera
Gaertn. (Lotus), an aquatic edible plant |
| title_short | Effects of NAA and BAP, double-layered media, and
light distance on in vitro regeneration of nelumbo nucifera
Gaertn. (Lotus), an aquatic edible plant |
| title_sort | effects of naa and bap, double-layered media, and
light distance on in vitro regeneration of nelumbo nucifera
gaertn. (lotus), an aquatic edible plant |
| topic | S Agriculture (General) SB Plant culture TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/37971/ http://irep.iium.edu.my/37971/ http://irep.iium.edu.my/37971/ http://irep.iium.edu.my/37971/1/SWJ.pdf http://irep.iium.edu.my/37971/4/37971_Effects%20of%20NAA%20and%20BAP_SCOPUS.pdf |