Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells
Background: Garcinia mangostana Linn. (GME) or mangosteen also known as ‘queen of fruits’ is a tropical plant and can be found in Southeast Asia region. The pericarps are utilized as traditional medicines to treat various ailments like abdominal pain, diarrhea, dysentery, infected wounds, suppurati...
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| Format: | Proceeding Paper |
| Language: | English English English |
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2014
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| Online Access: | http://irep.iium.edu.my/37838/ http://irep.iium.edu.my/37838/1/proceeding.pdf http://irep.iium.edu.my/37838/2/programbook.pdf http://irep.iium.edu.my/37838/3/poster_icip_faris.pdf |
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| author | Bakhtiar, M. Taher Tengku Zakaria, Tengku Muhamad Faris Syafiq |
| author_facet | Bakhtiar, M. Taher Tengku Zakaria, Tengku Muhamad Faris Syafiq |
| author_sort | Bakhtiar, M. Taher |
| building | IIUM Repository |
| collection | Online Access |
| description | Background: Garcinia mangostana Linn. (GME) or mangosteen also known as ‘queen of fruits’ is a tropical plant and can be found in Southeast Asia region. The pericarps are utilized as traditional medicines to treat various ailments like abdominal pain, diarrhea, dysentery, infected wounds,
suppuration and chronic ulcer attributed to secondary metabolites like xanthones (crenelated and oxygenated), tannins, anthocyanins, triterpenes, phenolics, polysaccharides, vitamins B1, B2 and C.
Aim: The present study was delineated to elucidate the effects of ethanolic extract (GME) on preadipocyte differentiation of 3T3-L1 cell and the possible glucose uptake mechanism signifying the potential to lower plasma glucose concentration in vivo.
Methods: Adipocyte cells were cultured in DMEM containing 1% penicillin–streptomycin (PS) and 10% fetal bovine serum (FBS) at 37oC in a humidified 5% CO2 atmosphere. Differentiation was initiated after 2-day post-confluent in MDI inducer medium (0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin)
for 2 days. The cells were then maintained another 2 days in 100 nM insulin and media was replaced thereafter for every 2 days prior to lipid formation. To examine the effects of GME on the
differentiation, cells were cultured in MDI differentiation medium in the presence of various concentrations of GME (2.5, 5.0 and 10.0 μg/ml).
Results: In 3T3-L1 cells, GME significantly enhanced adipogenic differentiation in a dose-dependent manner (p<0.05). GME also promoted glucose transport evidenced by uptake of Deoxy-D-glucose, 2-
[1,2-3H (N)]-in GME-treated cells (p<0.05). However, uptake of glucose was inhibited by 62.9% at highest concentration (10 μg/ml), suggesting inhibition of IRS-1 phosphorylation.
Conclusion: Our results suggested that mangosteen extract could be a potential therapeutic agent for
managing diabetes via promotion of adipogenesis with mild improvement in glucose utilization in vitro.
However, extensive study should be carried out to unravel the underlying molecular event lead to amelioration of diabetes complications. |
| first_indexed | 2025-11-14T15:50:16Z |
| format | Proceeding Paper |
| id | iium-37838 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T15:50:16Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-378382022-07-18T04:20:35Z http://irep.iium.edu.my/37838/ Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells Bakhtiar, M. Taher Tengku Zakaria, Tengku Muhamad Faris Syafiq RS Pharmacy and materia medica Background: Garcinia mangostana Linn. (GME) or mangosteen also known as ‘queen of fruits’ is a tropical plant and can be found in Southeast Asia region. The pericarps are utilized as traditional medicines to treat various ailments like abdominal pain, diarrhea, dysentery, infected wounds, suppuration and chronic ulcer attributed to secondary metabolites like xanthones (crenelated and oxygenated), tannins, anthocyanins, triterpenes, phenolics, polysaccharides, vitamins B1, B2 and C. Aim: The present study was delineated to elucidate the effects of ethanolic extract (GME) on preadipocyte differentiation of 3T3-L1 cell and the possible glucose uptake mechanism signifying the potential to lower plasma glucose concentration in vivo. Methods: Adipocyte cells were cultured in DMEM containing 1% penicillin–streptomycin (PS) and 10% fetal bovine serum (FBS) at 37oC in a humidified 5% CO2 atmosphere. Differentiation was initiated after 2-day post-confluent in MDI inducer medium (0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin) for 2 days. The cells were then maintained another 2 days in 100 nM insulin and media was replaced thereafter for every 2 days prior to lipid formation. To examine the effects of GME on the differentiation, cells were cultured in MDI differentiation medium in the presence of various concentrations of GME (2.5, 5.0 and 10.0 μg/ml). Results: In 3T3-L1 cells, GME significantly enhanced adipogenic differentiation in a dose-dependent manner (p<0.05). GME also promoted glucose transport evidenced by uptake of Deoxy-D-glucose, 2- [1,2-3H (N)]-in GME-treated cells (p<0.05). However, uptake of glucose was inhibited by 62.9% at highest concentration (10 μg/ml), suggesting inhibition of IRS-1 phosphorylation. Conclusion: Our results suggested that mangosteen extract could be a potential therapeutic agent for managing diabetes via promotion of adipogenesis with mild improvement in glucose utilization in vitro. However, extensive study should be carried out to unravel the underlying molecular event lead to amelioration of diabetes complications. 2014 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/37838/1/proceeding.pdf application/pdf en http://irep.iium.edu.my/37838/2/programbook.pdf application/pdf en http://irep.iium.edu.my/37838/3/poster_icip_faris.pdf Bakhtiar, M. Taher and Tengku Zakaria, Tengku Muhamad Faris Syafiq (2014) Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells. In: 1st International Conference on Industrial Pharmacy, 16-17 August 2014, Kuantan, Pahang. http://www.iium.edu.my/icip2014 |
| spellingShingle | RS Pharmacy and materia medica Bakhtiar, M. Taher Tengku Zakaria, Tengku Muhamad Faris Syafiq Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells |
| title | Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells |
| title_full | Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells |
| title_fullStr | Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells |
| title_full_unstemmed | Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells |
| title_short | Effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3T3-L1 cells |
| title_sort | effects of garcinia mangostana on adipocyte differentiation and glucose uptake regulation in 3t3-l1 cells |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/37838/ http://irep.iium.edu.my/37838/ http://irep.iium.edu.my/37838/1/proceeding.pdf http://irep.iium.edu.my/37838/2/programbook.pdf http://irep.iium.edu.my/37838/3/poster_icip_faris.pdf |