Suitability of using palm oil mill effluent as a medium for lipase production
Lipases are enzymes that can be secreted by several microorganisms using agro-industrial residues as potential substrates. This work screened ten microorganisms for their potential to produce lipase in palm oil mill effluent (POME)-based medium. Among the 10 organisms, the most promising strain wa...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Academic Journals
2011
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/3741/ http://irep.iium.edu.my/3741/1/Salihu_et_al_2011-AJB.pdf |
| _version_ | 1848776211036110848 |
|---|---|
| author | Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah |
| author_facet | Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah |
| author_sort | Salihu, Aliyu |
| building | IIUM Repository |
| collection | Online Access |
| description | Lipases are enzymes that can be secreted by several microorganisms using agro-industrial residues as
potential substrates. This work screened ten microorganisms for their potential to produce lipase in
palm oil mill effluent (POME)-based medium. Among the 10 organisms, the most promising strain was
Candida cylindracea (ATCC 14830) which showed appreciable activity both on agar plates and liquid
cultures. Medium supplementation by NH4Cl and olive oil led to an enzyme activity of 2.07 U/ml.
However, supplementation with organic nitrogen sources resulted in better enzyme activity. Addition of
malt extract, peptone and olive oil into the medium greatly influenced the lipase production. Among the
oils that were tested, olive oil was found to be the best for the expression of extracellular lipase at 0.5%
(v/v) with an activity of 4.02 U/ml in an optimized POME supplemented medium. |
| first_indexed | 2025-11-14T14:26:29Z |
| format | Article |
| id | iium-3741 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:26:29Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-37412021-07-06T02:07:44Z http://irep.iium.edu.my/3741/ Suitability of using palm oil mill effluent as a medium for lipase production Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah QD Chemistry TP248.13 Biotechnology Lipases are enzymes that can be secreted by several microorganisms using agro-industrial residues as potential substrates. This work screened ten microorganisms for their potential to produce lipase in palm oil mill effluent (POME)-based medium. Among the 10 organisms, the most promising strain was Candida cylindracea (ATCC 14830) which showed appreciable activity both on agar plates and liquid cultures. Medium supplementation by NH4Cl and olive oil led to an enzyme activity of 2.07 U/ml. However, supplementation with organic nitrogen sources resulted in better enzyme activity. Addition of malt extract, peptone and olive oil into the medium greatly influenced the lipase production. Among the oils that were tested, olive oil was found to be the best for the expression of extracellular lipase at 0.5% (v/v) with an activity of 4.02 U/ml in an optimized POME supplemented medium. Academic Journals 2011-03-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3741/1/Salihu_et_al_2011-AJB.pdf Salihu, Aliyu and Alam, Md Zahangir and AbdulKarim, M. Ismail and Mohd. Salleh, Hamzah (2011) Suitability of using palm oil mill effluent as a medium for lipase production. African Journal of Biotechnology, 10 (11). pp. 2044-2052. ISSN 1684–5315 http://www.academicjournals.org/AJB/PDF/pdf2011/14Mar/Salihu%20et%20al.pdf |
| spellingShingle | QD Chemistry TP248.13 Biotechnology Salihu, Aliyu Alam, Md Zahangir AbdulKarim, M. Ismail Mohd. Salleh, Hamzah Suitability of using palm oil mill effluent as a medium for lipase production |
| title | Suitability of using palm oil mill effluent as a medium
for lipase production |
| title_full | Suitability of using palm oil mill effluent as a medium
for lipase production |
| title_fullStr | Suitability of using palm oil mill effluent as a medium
for lipase production |
| title_full_unstemmed | Suitability of using palm oil mill effluent as a medium
for lipase production |
| title_short | Suitability of using palm oil mill effluent as a medium
for lipase production |
| title_sort | suitability of using palm oil mill effluent as a medium
for lipase production |
| topic | QD Chemistry TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/3741/ http://irep.iium.edu.my/3741/ http://irep.iium.edu.my/3741/1/Salihu_et_al_2011-AJB.pdf |