Isolation and characterization of antithrombin peptides from the saliva of Malaysian leeches
Purpose: To isolate and characterize the antithrombin compounds of Malaysian leeches’ saliva collection (LSC) for use as anticoagulant proteins and peptides. Method: Reversed phase - high performance liquid chromatography (RP-HPLC) was used to isolate all proteins from LSC. All isolated proteins...
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| Format: | Article |
| Language: | English English English |
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Pharmacotherapy Group, Faculty of Pharmacy, University of Benin
2014
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| Online Access: | http://irep.iium.edu.my/36520/ http://irep.iium.edu.my/36520/1/Isolation_and_Characterization_of_Antithrombin_Peptides.pdf http://irep.iium.edu.my/36520/4/36520_Isolation%20and%20characterization%20of%20antithrombin%20peptides_SCOPUS.pdf http://irep.iium.edu.my/36520/5/36520_Isolation%20and%20characterization%20of%20antithrombin%20peptides_WoS.pdf |
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| author | Alaama, Mohammed Helal Uddin, A.B.M. Mohammad Ghawi, Abbas Merzouk, Ahmed Abdualkader, Abdualrahman Mohammed Awang, Mohamed |
| author_facet | Alaama, Mohammed Helal Uddin, A.B.M. Mohammad Ghawi, Abbas Merzouk, Ahmed Abdualkader, Abdualrahman Mohammed Awang, Mohamed |
| author_sort | Alaama, Mohammed |
| building | IIUM Repository |
| collection | Online Access |
| description | Purpose: To isolate and characterize the antithrombin compounds of Malaysian leeches’ saliva
collection (LSC) for use as anticoagulant proteins and peptides.
Method: Reversed phase - high performance liquid chromatography (RP-HPLC) was used to isolate all
proteins from LSC. All isolated proteins were tested for anticoagulant activity by applying two tests,
thrombin time test and the inhibition of the amidolytic activity of thrombin on the chromogenic substrate
(S-2238). The molecular weights of isolated active compounds were identified using tricine sodium
dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE).
Results: More than 40 proteins were isolated from LSC using RP-HPLC. Two compounds (protein 1
and protein 2) were found to be active as they increased thrombin time by 30.26 and 36.75%,
respectively and inhibited the amidolytic activity of thrombin. as evaluated by measuring the conversion
of the chromogenic substrate (S-2238). Decrease in the conversion of the substrate (S-2238) by 31.10
and 41.61%, respectively was observed. The molecular weights of the proteins (6.289 and 14.255 kDa,
respectively) had no similarity with other leech species.
Conclusion: Two antithrombin proteins with anticoagulant properties were isolated from the saliva of
Malaysian leeches using one-step isolation procedure. The proteins did not show any similarity to
previous isolated proteins from leech. |
| first_indexed | 2025-11-14T15:46:30Z |
| format | Article |
| id | iium-36520 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T15:46:30Z |
| publishDate | 2014 |
| publisher | Pharmacotherapy Group, Faculty of Pharmacy, University of Benin |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-365202022-11-07T03:30:53Z http://irep.iium.edu.my/36520/ Isolation and characterization of antithrombin peptides from the saliva of Malaysian leeches Alaama, Mohammed Helal Uddin, A.B.M. Mohammad Ghawi, Abbas Merzouk, Ahmed Abdualkader, Abdualrahman Mohammed Awang, Mohamed QD Chemistry RS Pharmacy and materia medica Purpose: To isolate and characterize the antithrombin compounds of Malaysian leeches’ saliva collection (LSC) for use as anticoagulant proteins and peptides. Method: Reversed phase - high performance liquid chromatography (RP-HPLC) was used to isolate all proteins from LSC. All isolated proteins were tested for anticoagulant activity by applying two tests, thrombin time test and the inhibition of the amidolytic activity of thrombin on the chromogenic substrate (S-2238). The molecular weights of isolated active compounds were identified using tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS- PAGE). Results: More than 40 proteins were isolated from LSC using RP-HPLC. Two compounds (protein 1 and protein 2) were found to be active as they increased thrombin time by 30.26 and 36.75%, respectively and inhibited the amidolytic activity of thrombin. as evaluated by measuring the conversion of the chromogenic substrate (S-2238). Decrease in the conversion of the substrate (S-2238) by 31.10 and 41.61%, respectively was observed. The molecular weights of the proteins (6.289 and 14.255 kDa, respectively) had no similarity with other leech species. Conclusion: Two antithrombin proteins with anticoagulant properties were isolated from the saliva of Malaysian leeches using one-step isolation procedure. The proteins did not show any similarity to previous isolated proteins from leech. Pharmacotherapy Group, Faculty of Pharmacy, University of Benin 2014-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36520/1/Isolation_and_Characterization_of_Antithrombin_Peptides.pdf application/pdf en http://irep.iium.edu.my/36520/4/36520_Isolation%20and%20characterization%20of%20antithrombin%20peptides_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/36520/5/36520_Isolation%20and%20characterization%20of%20antithrombin%20peptides_WoS.pdf Alaama, Mohammed and Helal Uddin, A.B.M. and Mohammad Ghawi, Abbas and Merzouk, Ahmed and Abdualkader, Abdualrahman Mohammed and Awang, Mohamed (2014) Isolation and characterization of antithrombin peptides from the saliva of Malaysian leeches. Tropical Journal of Pharmaceutical Research, 13 (4). pp. 553-557. ISSN 1596-9827 (O), 1596-5996 (P) http://www.tjpr.org/vol13_no4/2014_13_4_10.php 10.4314/tjpr.v13i4.10 |
| spellingShingle | QD Chemistry RS Pharmacy and materia medica Alaama, Mohammed Helal Uddin, A.B.M. Mohammad Ghawi, Abbas Merzouk, Ahmed Abdualkader, Abdualrahman Mohammed Awang, Mohamed Isolation and characterization of antithrombin peptides from the saliva of Malaysian leeches |
| title | Isolation and characterization of antithrombin peptides
from the saliva of Malaysian leeches |
| title_full | Isolation and characterization of antithrombin peptides
from the saliva of Malaysian leeches |
| title_fullStr | Isolation and characterization of antithrombin peptides
from the saliva of Malaysian leeches |
| title_full_unstemmed | Isolation and characterization of antithrombin peptides
from the saliva of Malaysian leeches |
| title_short | Isolation and characterization of antithrombin peptides
from the saliva of Malaysian leeches |
| title_sort | isolation and characterization of antithrombin peptides
from the saliva of malaysian leeches |
| topic | QD Chemistry RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/36520/ http://irep.iium.edu.my/36520/ http://irep.iium.edu.my/36520/ http://irep.iium.edu.my/36520/1/Isolation_and_Characterization_of_Antithrombin_Peptides.pdf http://irep.iium.edu.my/36520/4/36520_Isolation%20and%20characterization%20of%20antithrombin%20peptides_SCOPUS.pdf http://irep.iium.edu.my/36520/5/36520_Isolation%20and%20characterization%20of%20antithrombin%20peptides_WoS.pdf |