Optimization of newcastle disease virus production in t-flask
In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on viru...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/3643/ http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf |
| _version_ | 1848776191219073024 |
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| author | Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah |
| author_facet | Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah |
| author_sort | Arifin, Mohd Azmir |
| building | IIUM Repository |
| collection | Online Access |
| description | In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used. |
| first_indexed | 2025-11-14T14:26:10Z |
| format | Proceeding Paper |
| id | iium-3643 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:26:10Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-36432012-01-19T00:25:56Z http://irep.iium.edu.my/3643/ Optimization of newcastle disease virus production in t-flask Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah QR355 Virology In this work, the propagation of Asplin F strain of Newcastle disease virus (NDV) in DF-1 cells was investigated. Experiments were carried out in T-flask to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on virus production. Virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used. 2011-05-17 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf Arifin, Mohd Azmir and Salim, Siti Hajar and Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Syed Hassan, Sharifah (2011) Optimization of newcastle disease virus production in t-flask. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | QR355 Virology Arifin, Mohd Azmir Salim, Siti Hajar Mel, Maizirwan Abdul Karim, Mohamed Ismail Syed Hassan, Sharifah Optimization of newcastle disease virus production in t-flask |
| title | Optimization of newcastle disease virus production in t-flask |
| title_full | Optimization of newcastle disease virus production in t-flask |
| title_fullStr | Optimization of newcastle disease virus production in t-flask |
| title_full_unstemmed | Optimization of newcastle disease virus production in t-flask |
| title_short | Optimization of newcastle disease virus production in t-flask |
| title_sort | optimization of newcastle disease virus production in t-flask |
| topic | QR355 Virology |
| url | http://irep.iium.edu.my/3643/ http://irep.iium.edu.my/3643/ http://irep.iium.edu.my/3643/1/Paper_NDV_Azmir.pdf |