α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells
α-Mangostin is a major compound found in mangosteen fruit hull and it has been reported to have wide range of pharmacological activities. The current study seeks to evaluate the in vitro activities of α-mangostin on adipocyte differentiation, glucose uptake and related gene expressions. The inhibit...
| Main Authors: | , , , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
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2014
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| Online Access: | http://irep.iium.edu.my/36403/ http://irep.iium.edu.my/36403/1/-PE_43_5FIP.pdf http://irep.iium.edu.my/36403/2/Binder1.pdf |
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| author | Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Zakaria, Zainul Amiruddin |
| author_facet | Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Zakaria, Zainul Amiruddin |
| author_sort | Mohamed Amiroudine, Mohamed Zaffar Ali |
| building | IIUM Repository |
| collection | Online Access |
| description | α-Mangostin is a major compound found in mangosteen fruit hull and it has been reported to have wide range of pharmacological activities. The current study seeks to evaluate the in vitro activities of α-mangostin on adipocyte differentiation, glucose uptake and related gene expressions. The inhibitory effect of the compound on lipid accumulation of 3T3-L1 preadipocytes was determined by Oil Red O staining method. Glucose uptake and free fatty acid release from the medium were determined by using 2-deoxy-D-[3H] glucose and adipolysis kit, respectively. Finally, the evaluation of related gene expression was carried out by the quantitative reverse transcriptase polymerase chain reaction (gRT-PCR). Depending on the dose, cell treated with α-mangostin was found to inhibit the cytoplasmic lipid accumulation as well as adipogenic differentiation of preadipocyte. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake (p<0.05). In addition, α-mangostin increased the amount of free fatty acid released. qRT-PCR analysis showed that the α-mangostin reduced lipid accumulation by inhibition of PPARγ gene expression. The induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. In summary, these evidences proposed that α-mangostin might be beneficial for obesity management.
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| first_indexed | 2025-11-14T15:46:09Z |
| format | Proceeding Paper |
| id | iium-36403 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T15:46:09Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-364032022-06-08T06:24:30Z http://irep.iium.edu.my/36403/ α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Zakaria, Zainul Amiruddin RS Pharmacy and materia medica α-Mangostin is a major compound found in mangosteen fruit hull and it has been reported to have wide range of pharmacological activities. The current study seeks to evaluate the in vitro activities of α-mangostin on adipocyte differentiation, glucose uptake and related gene expressions. The inhibitory effect of the compound on lipid accumulation of 3T3-L1 preadipocytes was determined by Oil Red O staining method. Glucose uptake and free fatty acid release from the medium were determined by using 2-deoxy-D-[3H] glucose and adipolysis kit, respectively. Finally, the evaluation of related gene expression was carried out by the quantitative reverse transcriptase polymerase chain reaction (gRT-PCR). Depending on the dose, cell treated with α-mangostin was found to inhibit the cytoplasmic lipid accumulation as well as adipogenic differentiation of preadipocyte. Analyses of 2-deoxy-D-[3H] glucose uptake activity showed that α-mangostin significantly improved the glucose uptake (p<0.05). In addition, α-mangostin increased the amount of free fatty acid released. qRT-PCR analysis showed that the α-mangostin reduced lipid accumulation by inhibition of PPARγ gene expression. The induction of glucose uptake and free fatty acid release by α-mangostin were accompanied by increasing mRNA expression of GLUT4 and leptin. In summary, these evidences proposed that α-mangostin might be beneficial for obesity management. 2014-04-13 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/36403/1/-PE_43_5FIP.pdf application/pdf en http://irep.iium.edu.my/36403/2/Binder1.pdf Mohamed Amiroudine, Mohamed Zaffar Ali and Bakhtiar, M. Taher and Darnis, Deny Susanti and Arief Ichwan, Solachuddin Jauhari and Kaderi, Mohd Arifin and Zakaria, Zainul Amiruddin (2014) α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells. In: 5th FIP Pharmaceutical Sciences World Congress (PSWC 2014), 13th-16th Apr. 2014, Melbourne, Australia. http://www.fip.org/pswc2014/Melbourne/2026/Abstracts/ |
| spellingShingle | RS Pharmacy and materia medica Mohamed Amiroudine, Mohamed Zaffar Ali Bakhtiar, M. Taher Darnis, Deny Susanti Arief Ichwan, Solachuddin Jauhari Kaderi, Mohd Arifin Zakaria, Zainul Amiruddin α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells |
| title | α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells |
| title_full | α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells |
| title_fullStr | α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells |
| title_full_unstemmed | α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells |
| title_short | α-Mangostin improves glucose uptake and inhibits adipocytes differentiation in 3T3-L1 cells |
| title_sort | α-mangostin improves glucose uptake and inhibits adipocytes differentiation in 3t3-l1 cells |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/36403/ http://irep.iium.edu.my/36403/ http://irep.iium.edu.my/36403/1/-PE_43_5FIP.pdf http://irep.iium.edu.my/36403/2/Binder1.pdf |