Comparing BRIN-BD11 culture producing insulin using different type of microcarriers
This research was conducted to examine the growth profile, growth kinetics, and insulinsecretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex� II) and crosslinked polystyrene Plastic...
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| Format: | Article |
| Language: | English |
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Springer
2010
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| Online Access: | http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/1/paper_at_cytotechnolopgy_%28dec_2010%29.pdf |
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| author | Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yusof, Siti Aishah Hashim, Yumi Zuhanis Has-Yun Ahmad Nor, Yusilawati |
| author_facet | Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yusof, Siti Aishah Hashim, Yumi Zuhanis Has-Yun Ahmad Nor, Yusilawati |
| author_sort | Mel, Maizirwan |
| building | IIUM Repository |
| collection | Online Access |
| description | This research was conducted to examine
the growth profile, growth kinetics, and insulinsecretory
responsiveness of BRIN-BD11 cells grown
in optimized medium on different types of microcarriers
(MCs). Comparisons were made on modified
polystyrene (Hillex� II) and crosslinked polystyrene
Plastic Plus (PP) from Solohill Engineering. The cell
line producing insulin was cultured in a 25 cm2 T-flask
as control while MCs based culture was implemented
in a stirred tank bioreactor with 1 L working volume.
For each culture type, the viable cell number, glucose,
lactate, glutamate, and insulin concentrations were
measured and compared. Maximum viable cell number
was obtained at 1.47 9 105 cell/mL for PP
microcarrier (PPMCs) culture, 1.35 9 105 cell/mL
Hillex� II (HIIMCs) culture and 0.95 9 105 cell/mL
for T-flask culture, respectively. The highest insulin
concentration has been produced in PPMCs culture
(5.31 mg/L) compared toHIIMCs culture (2.01 mg/L)
and T-flask culture (1.99 mg/L). Therefore overall
observation suggested that PPMCs was likely preferred
to be used for BRIN-BD11 cell culture as
compared with Hillex� II MCs. |
| first_indexed | 2025-11-14T14:26:09Z |
| format | Article |
| id | iium-3639 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:26:09Z |
| publishDate | 2010 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-36392011-09-22T03:54:04Z http://irep.iium.edu.my/3639/ Comparing BRIN-BD11 culture producing insulin using different type of microcarriers Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yusof, Siti Aishah Hashim, Yumi Zuhanis Has-Yun Ahmad Nor, Yusilawati TP155 Chemical engineering TP248.13 Biotechnology This research was conducted to examine the growth profile, growth kinetics, and insulinsecretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex� II) and crosslinked polystyrene Plastic Plus (PP) from Solohill Engineering. The cell line producing insulin was cultured in a 25 cm2 T-flask as control while MCs based culture was implemented in a stirred tank bioreactor with 1 L working volume. For each culture type, the viable cell number, glucose, lactate, glutamate, and insulin concentrations were measured and compared. Maximum viable cell number was obtained at 1.47 9 105 cell/mL for PP microcarrier (PPMCs) culture, 1.35 9 105 cell/mL Hillex� II (HIIMCs) culture and 0.95 9 105 cell/mL for T-flask culture, respectively. The highest insulin concentration has been produced in PPMCs culture (5.31 mg/L) compared toHIIMCs culture (2.01 mg/L) and T-flask culture (1.99 mg/L). Therefore overall observation suggested that PPMCs was likely preferred to be used for BRIN-BD11 cell culture as compared with Hillex� II MCs. Springer 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/3639/1/paper_at_cytotechnolopgy_%28dec_2010%29.pdf Mel, Maizirwan and Abdul Karim, Mohamed Ismail and Mohd Yusof, Siti Aishah and Hashim, Yumi Zuhanis Has-Yun and Ahmad Nor, Yusilawati (2010) Comparing BRIN-BD11 culture producing insulin using different type of microcarriers. Cytotechnology, 62. pp. 423-430. ISSN 1573-0778 (O), 0920-9069 (P) http://www.springerlink.com/content/h701w8l2x847h811/ DOI: 10.1007/s10616-010-9294-9 |
| spellingShingle | TP155 Chemical engineering TP248.13 Biotechnology Mel, Maizirwan Abdul Karim, Mohamed Ismail Mohd Yusof, Siti Aishah Hashim, Yumi Zuhanis Has-Yun Ahmad Nor, Yusilawati Comparing BRIN-BD11 culture producing insulin using different type of microcarriers |
| title | Comparing BRIN-BD11 culture producing insulin using different type of microcarriers |
| title_full | Comparing BRIN-BD11 culture producing insulin using different type of microcarriers |
| title_fullStr | Comparing BRIN-BD11 culture producing insulin using different type of microcarriers |
| title_full_unstemmed | Comparing BRIN-BD11 culture producing insulin using different type of microcarriers |
| title_short | Comparing BRIN-BD11 culture producing insulin using different type of microcarriers |
| title_sort | comparing brin-bd11 culture producing insulin using different type of microcarriers |
| topic | TP155 Chemical engineering TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/ http://irep.iium.edu.my/3639/1/paper_at_cytotechnolopgy_%28dec_2010%29.pdf |